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Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A - PLoS ONE (2015)

Bottom Line: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis.On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis.The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.

ABSTRACT
Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

No MeSH data available.


Related in: MedlinePlus

Co-cultured OCLs gene expression analysis.Relative quantification (2-ΔΔCt) of gene expression after 7 days of OCLs co-cultured with OBs grown in direct contact with all the tested samples. The graphs show the average and standard error of the technical triplicate of Oscar, Itgβ3 and CtsK, respect to the expression of the cells only, used as a control. Statistical significant difference among the samples is indicated in the graph: **p≤0.01.
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pone.0132633.g006: Co-cultured OCLs gene expression analysis.Relative quantification (2-ΔΔCt) of gene expression after 7 days of OCLs co-cultured with OBs grown in direct contact with all the tested samples. The graphs show the average and standard error of the technical triplicate of Oscar, Itgβ3 and CtsK, respect to the expression of the cells only, used as a control. Statistical significant difference among the samples is indicated in the graph: **p≤0.01.

Mentions: The quantification of co-cultured OCLs related gene shown that HA-LF and LF induced a significant down-regulation of Oscar (p<0.01) and CtsK, even if no statistical difference was found, compared to HA; on the contrary, HA-LF seems not exert any effects on Itgβ3 relative expression (Fig 6).


Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A - PLoS ONE (2015)

Co-cultured OCLs gene expression analysis.Relative quantification (2-ΔΔCt) of gene expression after 7 days of OCLs co-cultured with OBs grown in direct contact with all the tested samples. The graphs show the average and standard error of the technical triplicate of Oscar, Itgβ3 and CtsK, respect to the expression of the cells only, used as a control. Statistical significant difference among the samples is indicated in the graph: **p≤0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492779&req=5

pone.0132633.g006: Co-cultured OCLs gene expression analysis.Relative quantification (2-ΔΔCt) of gene expression after 7 days of OCLs co-cultured with OBs grown in direct contact with all the tested samples. The graphs show the average and standard error of the technical triplicate of Oscar, Itgβ3 and CtsK, respect to the expression of the cells only, used as a control. Statistical significant difference among the samples is indicated in the graph: **p≤0.01.
Mentions: The quantification of co-cultured OCLs related gene shown that HA-LF and LF induced a significant down-regulation of Oscar (p<0.01) and CtsK, even if no statistical difference was found, compared to HA; on the contrary, HA-LF seems not exert any effects on Itgβ3 relative expression (Fig 6).

Bottom Line: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis.On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis.The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.

ABSTRACT
Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

No MeSH data available.


Related in: MedlinePlus