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Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A - PLoS ONE (2015)

Bottom Line: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis.On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis.The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.

ABSTRACT
Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

No MeSH data available.


Related in: MedlinePlus

OCLs gene expression analysis.Relative quantification (2-ΔΔCt) of gene expression after 7 days of OCLs cultured in direct contact with all the tested samples. Average and standard error of the technical triplicate of Oscar, Itgβ3 and CtsK, respect to the expression of the cells only, were indicated. Statistical significant difference among the samples is indicated in the graph: *p≤0.05.
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pone.0132633.g004: OCLs gene expression analysis.Relative quantification (2-ΔΔCt) of gene expression after 7 days of OCLs cultured in direct contact with all the tested samples. Average and standard error of the technical triplicate of Oscar, Itgβ3 and CtsK, respect to the expression of the cells only, were indicated. Statistical significant difference among the samples is indicated in the graph: *p≤0.05.

Mentions: The quantification of OCLs related genes showed no significant differences in Oscar and CtsK relative expression among the tested samples (Fig 4). Conversely, the relative expression of Itgβ3 was significantly lower in HA-LF and LF compared to HA (p<0.05) (Fig 4).


Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A - PLoS ONE (2015)

OCLs gene expression analysis.Relative quantification (2-ΔΔCt) of gene expression after 7 days of OCLs cultured in direct contact with all the tested samples. Average and standard error of the technical triplicate of Oscar, Itgβ3 and CtsK, respect to the expression of the cells only, were indicated. Statistical significant difference among the samples is indicated in the graph: *p≤0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492779&req=5

pone.0132633.g004: OCLs gene expression analysis.Relative quantification (2-ΔΔCt) of gene expression after 7 days of OCLs cultured in direct contact with all the tested samples. Average and standard error of the technical triplicate of Oscar, Itgβ3 and CtsK, respect to the expression of the cells only, were indicated. Statistical significant difference among the samples is indicated in the graph: *p≤0.05.
Mentions: The quantification of OCLs related genes showed no significant differences in Oscar and CtsK relative expression among the tested samples (Fig 4). Conversely, the relative expression of Itgβ3 was significantly lower in HA-LF and LF compared to HA (p<0.05) (Fig 4).

Bottom Line: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis.On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis.The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.

ABSTRACT
Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

No MeSH data available.


Related in: MedlinePlus