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Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A - PLoS ONE (2015)

Bottom Line: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis.On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis.The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.

ABSTRACT
Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

No MeSH data available.


Related in: MedlinePlus

Multinucleated TRAP+ OCLs.The images show sRANKL-treated RAW264.7 cells cultured in direct contact with the samples: (A) HA-LF, (B) HA, (C) LF and (D) cells only, respectively. White arrows indicate large and multinucleated cells positive for tartrate-resistant acid phosphatase (mature OCLs). Scale bar 100 μm.
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pone.0132633.g003: Multinucleated TRAP+ OCLs.The images show sRANKL-treated RAW264.7 cells cultured in direct contact with the samples: (A) HA-LF, (B) HA, (C) LF and (D) cells only, respectively. White arrows indicate large and multinucleated cells positive for tartrate-resistant acid phosphatase (mature OCLs). Scale bar 100 μm.

Mentions: The qualitative evaluation of TRAP staining indicated that the presence of LF, both attached onto HA and free (Fig 3a and 3c), reduced the OCLs formation identifiable as large multinucleated cells positive for TRAP activity, compared to HA and cells only samples (Fig 3b and 3d).


Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A - PLoS ONE (2015)

Multinucleated TRAP+ OCLs.The images show sRANKL-treated RAW264.7 cells cultured in direct contact with the samples: (A) HA-LF, (B) HA, (C) LF and (D) cells only, respectively. White arrows indicate large and multinucleated cells positive for tartrate-resistant acid phosphatase (mature OCLs). Scale bar 100 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492779&req=5

pone.0132633.g003: Multinucleated TRAP+ OCLs.The images show sRANKL-treated RAW264.7 cells cultured in direct contact with the samples: (A) HA-LF, (B) HA, (C) LF and (D) cells only, respectively. White arrows indicate large and multinucleated cells positive for tartrate-resistant acid phosphatase (mature OCLs). Scale bar 100 μm.
Mentions: The qualitative evaluation of TRAP staining indicated that the presence of LF, both attached onto HA and free (Fig 3a and 3c), reduced the OCLs formation identifiable as large multinucleated cells positive for TRAP activity, compared to HA and cells only samples (Fig 3b and 3d).

Bottom Line: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis.On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis.The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.

ABSTRACT
Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

No MeSH data available.


Related in: MedlinePlus