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Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A - PLoS ONE (2015)

Bottom Line: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis.On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis.The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.

ABSTRACT
Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

No MeSH data available.


Related in: MedlinePlus

OBs gene expression analysis.(a) and (c) Relative quantification (2-ΔΔCt) of gene expression after 7 and (b) and (d) 14 days of OBs cultured in direct contact with all the tested samples. The graph shows the average and standard error of the technical triplicate of Osterix and IBSP, respect to the expression of the cells only, used as a control. Statistical significant differences among the samples are indicated in the graphs: *p≤0.05 and ***p≤0.001.
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pone.0132633.g002: OBs gene expression analysis.(a) and (c) Relative quantification (2-ΔΔCt) of gene expression after 7 and (b) and (d) 14 days of OBs cultured in direct contact with all the tested samples. The graph shows the average and standard error of the technical triplicate of Osterix and IBSP, respect to the expression of the cells only, used as a control. Statistical significant differences among the samples are indicated in the graphs: *p≤0.05 and ***p≤0.001.

Mentions: The quantification of Osterix and IBSP mRNA level showed that after 7 days cells expressed a basal, or lower level of both the genes compared to the cells only (Fig 2a and 2c). Instead, after 14 days, HA-LF significantly upregulated the expression of both these genes (Fig 2b and 2d); in detail, the sample HA-LF significantly increased the Osterix relative expression compared to HA sample (p<0.05) and the IBSP relative expression compared to both HA and LF samples (p<0.001).


Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A - PLoS ONE (2015)

OBs gene expression analysis.(a) and (c) Relative quantification (2-ΔΔCt) of gene expression after 7 and (b) and (d) 14 days of OBs cultured in direct contact with all the tested samples. The graph shows the average and standard error of the technical triplicate of Osterix and IBSP, respect to the expression of the cells only, used as a control. Statistical significant differences among the samples are indicated in the graphs: *p≤0.05 and ***p≤0.001.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492779&req=5

pone.0132633.g002: OBs gene expression analysis.(a) and (c) Relative quantification (2-ΔΔCt) of gene expression after 7 and (b) and (d) 14 days of OBs cultured in direct contact with all the tested samples. The graph shows the average and standard error of the technical triplicate of Osterix and IBSP, respect to the expression of the cells only, used as a control. Statistical significant differences among the samples are indicated in the graphs: *p≤0.05 and ***p≤0.001.
Mentions: The quantification of Osterix and IBSP mRNA level showed that after 7 days cells expressed a basal, or lower level of both the genes compared to the cells only (Fig 2a and 2c). Instead, after 14 days, HA-LF significantly upregulated the expression of both these genes (Fig 2b and 2d); in detail, the sample HA-LF significantly increased the Osterix relative expression compared to HA sample (p<0.05) and the IBSP relative expression compared to both HA and LF samples (p<0.001).

Bottom Line: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis.On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis.The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.

ABSTRACT
Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

No MeSH data available.


Related in: MedlinePlus