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Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A - PLoS ONE (2015)

Bottom Line: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis.On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis.The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.

ABSTRACT
Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

No MeSH data available.


Related in: MedlinePlus

OBs viability and apoptosis.(A) shows the percentage of live OBs respect to the total cells counted and (B) shows the percentage of apoptotic OBs respect to the total cells counted. Mean and standard error (n = 3) represented as the percentage of the total counted cells, after 7 and 14 days of culture in direct contact with all the tested samples. Statistical significant differences among the samples are indicated in both graphs: *p≤0.05. (C) Different examples of nuclear fragmentation in OBs stained with DAPI are indicated with red arrows. Scale bar 50 μm.
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pone.0132633.g001: OBs viability and apoptosis.(A) shows the percentage of live OBs respect to the total cells counted and (B) shows the percentage of apoptotic OBs respect to the total cells counted. Mean and standard error (n = 3) represented as the percentage of the total counted cells, after 7 and 14 days of culture in direct contact with all the tested samples. Statistical significant differences among the samples are indicated in both graphs: *p≤0.05. (C) Different examples of nuclear fragmentation in OBs stained with DAPI are indicated with red arrows. Scale bar 50 μm.

Mentions: The ratio of live cells showed no significant difference among all the samples tested except for HA at day 7 that showed a statistical significant decrease of live cells compared to all the other samples (p<0.05) (Fig 1A). After 14 days of culture this difference disappeared and the percentage of cells live revealed no differences among the samples and the cells only used as control (Fig 1a). Moreover, also the percentage of apoptotic OBs was significant higher in HA sample after 7 days of culture (p <0.05) compared to all the samples tested (Fig 1b and 1c). After 14 days of culture apoptotic cells in HA samples decreased, while no changing were found in the other samples (Fig 1b).


Coupling Hydroxyapatite Nanocrystals with Lactoferrin as a Promising Strategy to Fine Regulate Bone Homeostasis.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A - PLoS ONE (2015)

OBs viability and apoptosis.(A) shows the percentage of live OBs respect to the total cells counted and (B) shows the percentage of apoptotic OBs respect to the total cells counted. Mean and standard error (n = 3) represented as the percentage of the total counted cells, after 7 and 14 days of culture in direct contact with all the tested samples. Statistical significant differences among the samples are indicated in both graphs: *p≤0.05. (C) Different examples of nuclear fragmentation in OBs stained with DAPI are indicated with red arrows. Scale bar 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492779&req=5

pone.0132633.g001: OBs viability and apoptosis.(A) shows the percentage of live OBs respect to the total cells counted and (B) shows the percentage of apoptotic OBs respect to the total cells counted. Mean and standard error (n = 3) represented as the percentage of the total counted cells, after 7 and 14 days of culture in direct contact with all the tested samples. Statistical significant differences among the samples are indicated in both graphs: *p≤0.05. (C) Different examples of nuclear fragmentation in OBs stained with DAPI are indicated with red arrows. Scale bar 50 μm.
Mentions: The ratio of live cells showed no significant difference among all the samples tested except for HA at day 7 that showed a statistical significant decrease of live cells compared to all the other samples (p<0.05) (Fig 1A). After 14 days of culture this difference disappeared and the percentage of cells live revealed no differences among the samples and the cells only used as control (Fig 1a). Moreover, also the percentage of apoptotic OBs was significant higher in HA sample after 7 days of culture (p <0.05) compared to all the samples tested (Fig 1b and 1c). After 14 days of culture apoptotic cells in HA samples decreased, while no changing were found in the other samples (Fig 1b).

Bottom Line: Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis.On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis.The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

View Article: PubMed Central - PubMed

Affiliation: Institute of Science and Technology for Ceramics, National Research Council, Faenza, Ravenna, Italy.

ABSTRACT
Lactoferrin (LF) is an interesting glycoprotein in the field of bone biology for its regulatory effect on cells involved in bone remodeling, that results compromised in several pathological conditions, as osteoporosis. In a previous study we observed that the coupling of LF and biomimetic hydroxyapatite nanocrystals (HA), a material well-known for its bioactivity and osteoconductive properties, leads to a combined effect in the induction of osteogenic differentiation of mesenchymal stem cells. On the basis of this evidence, the present study is an extension of our previous work aiming to investigate the synergistic effect of the coupling of HA and LF on bone homeostasis. Biomimetic HA nanocrystals were synthesized and functionalized with LF (HA-LF) and then pre-osteoblasts (MC3T3-E1) and monocyte/macrophage cells lines (RAW 264.7), using as osteoclastogenesis in vitro model, were cultured separately or in co-culture in presence of HA-LF. The results clearly revealed that HA and LF act in synergism in the regulation of the bone homeostasis, working as anabolic factor for osteoblasts differentiation and bone matrix deposition, and as inhibitor of the osteoclast formation and activity.

No MeSH data available.


Related in: MedlinePlus