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Changes in Estrogen Receptor ERβ (ESR2) Expression without Changes in the Estradiol Levels in the Prostate of Aging Rats.

Morais-Santos M, Nunes AE, Oliveira AG, Moura-Cordeiro JD, Mahecha GA, Avellar MC, Oliveira CA - PLoS ONE (2015)

Bottom Line: Despite the relationship between reductions in ERβ and abnormal growth of the gland, little is known about the age-dependent variation of this receptor.The decrease in ERβ reactivity occurred in a hormonal milieu characterized by a constant concentration of estradiol and decreased plasmatic and tissue DHT.These data corroborate previous data from humans and dogs that silencing of this receptor may be associated with premalignant or malignant conditions in the prostate.

View Article: PubMed Central - PubMed

Affiliation: Department of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Although the prostate is androgen-dependent, it is also influenced by estrogens, which act via the estrogen receptors ERα and ERβ. In the prostate, ERβ is highly expressed in the epithelium and appears to participate in the regulation of cell proliferation, apoptosis and differentiation. Evidence shows that ERβ is decreased in malignant prostate, suggesting that it plays an important role in protecting this tissue. Despite the relationship between reductions in ERβ and abnormal growth of the gland, little is known about the age-dependent variation of this receptor. Therefore, we aimed to investigate ERβ expression in the prostatic lobes of aging Wistar rats (3 to 24 months). Histopathological alterations, including hyperplasia, intraluminal concretions, nuclear atypia and prostate intraepithelial neoplasias (PIN), were observed in the prostates of aging rats. Epithelial proliferation led to cribriform architecture in some acini, especially in the ventral prostate (VP). In the VP, areas of epithelial atrophy were also observed. Furthermore, in the lateral prostate, there was frequent prostatitis. Immunohistochemistry revealed that the expression of ERβ is reduced in specific areas related to PIN, atrophic abnormalities and cellular atypia in the prostate epithelium of senile rats. Corroborating the involvement of the receptor with proliferative activity, the punctual reduction in ERβ paralleled the increase in cell proliferation especially in areas of PIN and nuclear atypies. The decrease in ERβ reactivity occurred in a hormonal milieu characterized by a constant concentration of estradiol and decreased plasmatic and tissue DHT. This paper is a pioneering study that reveals focal ERβ reduction in the prostate of aging rats and indicates a potential disorder in the ERβ pathway. These data corroborate previous data from humans and dogs that silencing of this receptor may be associated with premalignant or malignant conditions in the prostate.

No MeSH data available.


Related in: MedlinePlus

Western blotting of ERβ in the rat prostates at different ages.(A, C, E and G) Representative bands from the assay. β-actin was used as a reference. (B, D, F and H) Graphical representation of the band densitometric analysis. The data shown are representative of two to three different assays. VP = ventral prostate; DP = dorsal prostate; LP = lateral prostate and AP = anterior prostate. n = 5 per group.
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pone.0131901.g004: Western blotting of ERβ in the rat prostates at different ages.(A, C, E and G) Representative bands from the assay. β-actin was used as a reference. (B, D, F and H) Graphical representation of the band densitometric analysis. The data shown are representative of two to three different assays. VP = ventral prostate; DP = dorsal prostate; LP = lateral prostate and AP = anterior prostate. n = 5 per group.

Mentions: The specificity of the antibody was confirmed according to the detection of a principal band of 54 kDa within the expected molecular mass previously described for rat ERβ [3, 35]. A second reactive band of 49 kDa, however, co-migrated with the principal band (Fig 4). The pixel intensity of the ERβ bands was similar among adult and senile rats (Fig 4A–4H) as well as among the different lobes (Fig 4I and 4J).


Changes in Estrogen Receptor ERβ (ESR2) Expression without Changes in the Estradiol Levels in the Prostate of Aging Rats.

Morais-Santos M, Nunes AE, Oliveira AG, Moura-Cordeiro JD, Mahecha GA, Avellar MC, Oliveira CA - PLoS ONE (2015)

Western blotting of ERβ in the rat prostates at different ages.(A, C, E and G) Representative bands from the assay. β-actin was used as a reference. (B, D, F and H) Graphical representation of the band densitometric analysis. The data shown are representative of two to three different assays. VP = ventral prostate; DP = dorsal prostate; LP = lateral prostate and AP = anterior prostate. n = 5 per group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492744&req=5

pone.0131901.g004: Western blotting of ERβ in the rat prostates at different ages.(A, C, E and G) Representative bands from the assay. β-actin was used as a reference. (B, D, F and H) Graphical representation of the band densitometric analysis. The data shown are representative of two to three different assays. VP = ventral prostate; DP = dorsal prostate; LP = lateral prostate and AP = anterior prostate. n = 5 per group.
Mentions: The specificity of the antibody was confirmed according to the detection of a principal band of 54 kDa within the expected molecular mass previously described for rat ERβ [3, 35]. A second reactive band of 49 kDa, however, co-migrated with the principal band (Fig 4). The pixel intensity of the ERβ bands was similar among adult and senile rats (Fig 4A–4H) as well as among the different lobes (Fig 4I and 4J).

Bottom Line: Despite the relationship between reductions in ERβ and abnormal growth of the gland, little is known about the age-dependent variation of this receptor.The decrease in ERβ reactivity occurred in a hormonal milieu characterized by a constant concentration of estradiol and decreased plasmatic and tissue DHT.These data corroborate previous data from humans and dogs that silencing of this receptor may be associated with premalignant or malignant conditions in the prostate.

View Article: PubMed Central - PubMed

Affiliation: Department of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.

ABSTRACT
Although the prostate is androgen-dependent, it is also influenced by estrogens, which act via the estrogen receptors ERα and ERβ. In the prostate, ERβ is highly expressed in the epithelium and appears to participate in the regulation of cell proliferation, apoptosis and differentiation. Evidence shows that ERβ is decreased in malignant prostate, suggesting that it plays an important role in protecting this tissue. Despite the relationship between reductions in ERβ and abnormal growth of the gland, little is known about the age-dependent variation of this receptor. Therefore, we aimed to investigate ERβ expression in the prostatic lobes of aging Wistar rats (3 to 24 months). Histopathological alterations, including hyperplasia, intraluminal concretions, nuclear atypia and prostate intraepithelial neoplasias (PIN), were observed in the prostates of aging rats. Epithelial proliferation led to cribriform architecture in some acini, especially in the ventral prostate (VP). In the VP, areas of epithelial atrophy were also observed. Furthermore, in the lateral prostate, there was frequent prostatitis. Immunohistochemistry revealed that the expression of ERβ is reduced in specific areas related to PIN, atrophic abnormalities and cellular atypia in the prostate epithelium of senile rats. Corroborating the involvement of the receptor with proliferative activity, the punctual reduction in ERβ paralleled the increase in cell proliferation especially in areas of PIN and nuclear atypies. The decrease in ERβ reactivity occurred in a hormonal milieu characterized by a constant concentration of estradiol and decreased plasmatic and tissue DHT. This paper is a pioneering study that reveals focal ERβ reduction in the prostate of aging rats and indicates a potential disorder in the ERβ pathway. These data corroborate previous data from humans and dogs that silencing of this receptor may be associated with premalignant or malignant conditions in the prostate.

No MeSH data available.


Related in: MedlinePlus