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Honey Bees (Apis mellifera, L.) as Active Samplers of Airborne Particulate Matter.

Negri I, Mavris C, Di Prisco G, Caprio E, Pellecchia M - PLoS ONE (2015)

Bottom Line: In the present work we demonstrate that bees can also be used as active samplers of airborne particulate matter.The analyses pointed to specific morphological and chemical features of the particulate, and resulted into the identification of three categories of particles: industry-, postmining-, and soil-derived.With the exception of the gut, all the analyzed body districts displayed inorganic particles, mostly concentrated in specific areas of the body (i.e. along the costal margin of the fore wings, the medial plane of the head, and the inner surface of the hind legs).

View Article: PubMed Central - PubMed

Affiliation: Koiné-Environmental Consulting S.n.c., Parma, Italy.

ABSTRACT
Honey bees (Apis mellifera L.) are bioindicators of environmental pollution levels. During their wide-ranging foraging activity, these hymenopterans are exposed to pollutants, thus becoming a useful tool to trace the environmental contaminants as heavy metals, pesticides, radionuclides and volatile organic compounds. In the present work we demonstrate that bees can also be used as active samplers of airborne particulate matter. Worker bees were collected from hives located in a polluted postmining area in South West Sardinia (Italy) that is also exposed to dust emissions from industrial plants. The area is included in an official list of sites of national interest for environmental remediation, and has been characterized for the effects of pollutants on the health of the resident population. The head, wings, hind legs and alimentary canal of the bees were investigated with Scanning Electron Microscopy coupled with X-ray spectroscopy (SEM-EDX). The analyses pointed to specific morphological and chemical features of the particulate, and resulted into the identification of three categories of particles: industry-, postmining-, and soil-derived. With the exception of the gut, all the analyzed body districts displayed inorganic particles, mostly concentrated in specific areas of the body (i.e. along the costal margin of the fore wings, the medial plane of the head, and the inner surface of the hind legs). The role of both past mining activities and the industrial activity close to the study area as sources of the particulate matter is also discussed. We conclude that honey bees are able to collect samples of the main airborne particles emitted from different sources, therefore could be an ideal tool for monitoring such a kind of pollutants.

No MeSH data available.


Related in: MedlinePlus

BSE images of PM (bright spots) on the hind legs. (A) Metatarsus.Bar = 1 mm. (B) Distal tarsal segments. Bar = 150 μm. (C) Detail of the structures involved in the grooming behavior and pollen collection. The pecten spines (arrow) and the pyramidal spines of the auricle (arrowhead) convey and pack the pollen into the pollen basket located on the outer surface of the leg. The pollen comb (pc), composed by transverse rows of stiff spines, brush off pollen from the lateral surface of the body and collect wax scales from the abdomen. Bar = 300 μm.
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pone.0132491.g005: BSE images of PM (bright spots) on the hind legs. (A) Metatarsus.Bar = 1 mm. (B) Distal tarsal segments. Bar = 150 μm. (C) Detail of the structures involved in the grooming behavior and pollen collection. The pecten spines (arrow) and the pyramidal spines of the auricle (arrowhead) convey and pack the pollen into the pollen basket located on the outer surface of the leg. The pollen comb (pc), composed by transverse rows of stiff spines, brush off pollen from the lateral surface of the body and collect wax scales from the abdomen. Bar = 300 μm.

Mentions: On the third pairs of legs, the coverage of inorganic particles was always rather diffused along the most distal segments of the inner surface, and involved the structures dedicated to the body grooming, pollen collection, and wax handling (e.g. the pecten on the lower end of the tibia and the pollen comb on the metatarsus) (Figs 2A and 5).


Honey Bees (Apis mellifera, L.) as Active Samplers of Airborne Particulate Matter.

Negri I, Mavris C, Di Prisco G, Caprio E, Pellecchia M - PLoS ONE (2015)

BSE images of PM (bright spots) on the hind legs. (A) Metatarsus.Bar = 1 mm. (B) Distal tarsal segments. Bar = 150 μm. (C) Detail of the structures involved in the grooming behavior and pollen collection. The pecten spines (arrow) and the pyramidal spines of the auricle (arrowhead) convey and pack the pollen into the pollen basket located on the outer surface of the leg. The pollen comb (pc), composed by transverse rows of stiff spines, brush off pollen from the lateral surface of the body and collect wax scales from the abdomen. Bar = 300 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492680&req=5

pone.0132491.g005: BSE images of PM (bright spots) on the hind legs. (A) Metatarsus.Bar = 1 mm. (B) Distal tarsal segments. Bar = 150 μm. (C) Detail of the structures involved in the grooming behavior and pollen collection. The pecten spines (arrow) and the pyramidal spines of the auricle (arrowhead) convey and pack the pollen into the pollen basket located on the outer surface of the leg. The pollen comb (pc), composed by transverse rows of stiff spines, brush off pollen from the lateral surface of the body and collect wax scales from the abdomen. Bar = 300 μm.
Mentions: On the third pairs of legs, the coverage of inorganic particles was always rather diffused along the most distal segments of the inner surface, and involved the structures dedicated to the body grooming, pollen collection, and wax handling (e.g. the pecten on the lower end of the tibia and the pollen comb on the metatarsus) (Figs 2A and 5).

Bottom Line: In the present work we demonstrate that bees can also be used as active samplers of airborne particulate matter.The analyses pointed to specific morphological and chemical features of the particulate, and resulted into the identification of three categories of particles: industry-, postmining-, and soil-derived.With the exception of the gut, all the analyzed body districts displayed inorganic particles, mostly concentrated in specific areas of the body (i.e. along the costal margin of the fore wings, the medial plane of the head, and the inner surface of the hind legs).

View Article: PubMed Central - PubMed

Affiliation: Koiné-Environmental Consulting S.n.c., Parma, Italy.

ABSTRACT
Honey bees (Apis mellifera L.) are bioindicators of environmental pollution levels. During their wide-ranging foraging activity, these hymenopterans are exposed to pollutants, thus becoming a useful tool to trace the environmental contaminants as heavy metals, pesticides, radionuclides and volatile organic compounds. In the present work we demonstrate that bees can also be used as active samplers of airborne particulate matter. Worker bees were collected from hives located in a polluted postmining area in South West Sardinia (Italy) that is also exposed to dust emissions from industrial plants. The area is included in an official list of sites of national interest for environmental remediation, and has been characterized for the effects of pollutants on the health of the resident population. The head, wings, hind legs and alimentary canal of the bees were investigated with Scanning Electron Microscopy coupled with X-ray spectroscopy (SEM-EDX). The analyses pointed to specific morphological and chemical features of the particulate, and resulted into the identification of three categories of particles: industry-, postmining-, and soil-derived. With the exception of the gut, all the analyzed body districts displayed inorganic particles, mostly concentrated in specific areas of the body (i.e. along the costal margin of the fore wings, the medial plane of the head, and the inner surface of the hind legs). The role of both past mining activities and the industrial activity close to the study area as sources of the particulate matter is also discussed. We conclude that honey bees are able to collect samples of the main airborne particles emitted from different sources, therefore could be an ideal tool for monitoring such a kind of pollutants.

No MeSH data available.


Related in: MedlinePlus