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Physiological Evaluation of Alkali-Salt Tolerance of Thirty Switchgrass (Panicum virgatum) Lines.

Hu G, Liu Y, Zhang X, Yao F, Huang Y, Ervin EH, Zhao B - PLoS ONE (2015)

Bottom Line: Significant differences in alkali-salt stress tolerance were found among the 30 lines.The results suggest wide variations exist in alkali-salt stress tolerance among the 30 switchgrass lines.The approach of using a combination of principal components and cluster analysis of the physiological parameters and related ASTTI is feasible for evaluating alkali-salt tolerance in switchgrass.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang Province, P.R. China; Department of Crop and Soil Environmental Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America.

ABSTRACT
Soil salt-alkalization is a major limiting factor for crop production in many regions. Switchgrass (Panicum virgatum L.) is a warm-season C4 perennial rhizomatous bunchgrass and a target lignocellulosic biofuel species. The objective of this study was to evaluate relative alkali-salt tolerance among 30 switchgrass lines. Tillers of each switchgrass line were transplanted into pots filled with fine sand. Two months after transplanting, plants at E5 developmental stage were grown in either half strength Hoagland's nutrient solution with 0 mM Na+ (control) or half strength Hoagland's nutrient solution with 150 mM Na+ and pH of 9.5 (alkali-salt stress treatment) for 20 d. Alkali-salt stress damaged cell membranes [higher electrolyte leakage (EL)], reduced leaf relative water content (RWC), net photosynthetic rate (Pn), stomatal conductance (gs), and transpiration rate (Tr). An alkali-salt stress tolerance trait index (ASTTI) for each parameter was calculated based on the ratio of the value under alkali-salt stress and the value under non-stress conditions for each parameter of each line. Relative alkali-salt tolerance was determined based on principal components analysis and cluster analysis of the physiological parameters and their ASTTI values. Significant differences in alkali-salt stress tolerance were found among the 30 lines. Lowland lines TEM-SEC, Alamo, TEM-SLC and Kanlow were classified as alkali-salt tolerant. In contrast, three lowland lines (AM-314/MS-155, BN-13645-64) and two upland lines (Caddo and Blackwell-1) were classified as alkali-salt sensitive. The results suggest wide variations exist in alkali-salt stress tolerance among the 30 switchgrass lines. The approach of using a combination of principal components and cluster analysis of the physiological parameters and related ASTTI is feasible for evaluating alkali-salt tolerance in switchgrass.

No MeSH data available.


Related in: MedlinePlus

Clusters analysis of five physiological parameters in 30 switchgrass lines.
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pone.0125305.g006: Clusters analysis of five physiological parameters in 30 switchgrass lines.

Mentions: The physiological parameters in 30 switchgrass lines were used for a cluster analysis. As shown in Fig 6, the experimental lines fell into three categories: Category I (five lines), including TEM-SEC, Kanlow, 70SG0023, Alamo and TEM-SLC, had the lowest level of overall EL and the highest levels of overall Pn, gs and Tr (that is, the highest level of photosynthetic index). These lines accounted for 16.67% of total experiment lines and were considered as alkali-salt stress tolerant lines. Category II (18 lines), including Grif Nebraska 28,70SG0019, Pathfinder, Blackwell-3, BN-8624-67, Cave-in-Rock, T16971, 70SG001, BN-12323-69, 70SG002, BN-18758-67, 70SG0018, TEM-LoDorm, T-2086, Sunburst, BN-11357-63, Grenville-2, and BN-10860-61, had medium levels of all physiological indices. These lines accounted for 60% of total experiment lines. Category III (7 lines), including Caddo, Summer, 70SG0022, AM-314/MS-155, Blackwell-1, BN-13645-64 and Dacotah, had the lowest levels of overall physiological indices and therefore the least tolerant to alkali-salt stress. These lines accounted for 23.3% of total experiment lines and were considered as alkali-salt sensitive lines. Overall, the alkali-salt stress tolerance of three categories was ranked as: Category I > Category II > Category III.


Physiological Evaluation of Alkali-Salt Tolerance of Thirty Switchgrass (Panicum virgatum) Lines.

Hu G, Liu Y, Zhang X, Yao F, Huang Y, Ervin EH, Zhao B - PLoS ONE (2015)

Clusters analysis of five physiological parameters in 30 switchgrass lines.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492678&req=5

pone.0125305.g006: Clusters analysis of five physiological parameters in 30 switchgrass lines.
Mentions: The physiological parameters in 30 switchgrass lines were used for a cluster analysis. As shown in Fig 6, the experimental lines fell into three categories: Category I (five lines), including TEM-SEC, Kanlow, 70SG0023, Alamo and TEM-SLC, had the lowest level of overall EL and the highest levels of overall Pn, gs and Tr (that is, the highest level of photosynthetic index). These lines accounted for 16.67% of total experiment lines and were considered as alkali-salt stress tolerant lines. Category II (18 lines), including Grif Nebraska 28,70SG0019, Pathfinder, Blackwell-3, BN-8624-67, Cave-in-Rock, T16971, 70SG001, BN-12323-69, 70SG002, BN-18758-67, 70SG0018, TEM-LoDorm, T-2086, Sunburst, BN-11357-63, Grenville-2, and BN-10860-61, had medium levels of all physiological indices. These lines accounted for 60% of total experiment lines. Category III (7 lines), including Caddo, Summer, 70SG0022, AM-314/MS-155, Blackwell-1, BN-13645-64 and Dacotah, had the lowest levels of overall physiological indices and therefore the least tolerant to alkali-salt stress. These lines accounted for 23.3% of total experiment lines and were considered as alkali-salt sensitive lines. Overall, the alkali-salt stress tolerance of three categories was ranked as: Category I > Category II > Category III.

Bottom Line: Significant differences in alkali-salt stress tolerance were found among the 30 lines.The results suggest wide variations exist in alkali-salt stress tolerance among the 30 switchgrass lines.The approach of using a combination of principal components and cluster analysis of the physiological parameters and related ASTTI is feasible for evaluating alkali-salt tolerance in switchgrass.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang Province, P.R. China; Department of Crop and Soil Environmental Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America.

ABSTRACT
Soil salt-alkalization is a major limiting factor for crop production in many regions. Switchgrass (Panicum virgatum L.) is a warm-season C4 perennial rhizomatous bunchgrass and a target lignocellulosic biofuel species. The objective of this study was to evaluate relative alkali-salt tolerance among 30 switchgrass lines. Tillers of each switchgrass line were transplanted into pots filled with fine sand. Two months after transplanting, plants at E5 developmental stage were grown in either half strength Hoagland's nutrient solution with 0 mM Na+ (control) or half strength Hoagland's nutrient solution with 150 mM Na+ and pH of 9.5 (alkali-salt stress treatment) for 20 d. Alkali-salt stress damaged cell membranes [higher electrolyte leakage (EL)], reduced leaf relative water content (RWC), net photosynthetic rate (Pn), stomatal conductance (gs), and transpiration rate (Tr). An alkali-salt stress tolerance trait index (ASTTI) for each parameter was calculated based on the ratio of the value under alkali-salt stress and the value under non-stress conditions for each parameter of each line. Relative alkali-salt tolerance was determined based on principal components analysis and cluster analysis of the physiological parameters and their ASTTI values. Significant differences in alkali-salt stress tolerance were found among the 30 lines. Lowland lines TEM-SEC, Alamo, TEM-SLC and Kanlow were classified as alkali-salt tolerant. In contrast, three lowland lines (AM-314/MS-155, BN-13645-64) and two upland lines (Caddo and Blackwell-1) were classified as alkali-salt sensitive. The results suggest wide variations exist in alkali-salt stress tolerance among the 30 switchgrass lines. The approach of using a combination of principal components and cluster analysis of the physiological parameters and related ASTTI is feasible for evaluating alkali-salt tolerance in switchgrass.

No MeSH data available.


Related in: MedlinePlus