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Physiological Evaluation of Alkali-Salt Tolerance of Thirty Switchgrass (Panicum virgatum) Lines.

Hu G, Liu Y, Zhang X, Yao F, Huang Y, Ervin EH, Zhao B - PLoS ONE (2015)

Bottom Line: Significant differences in alkali-salt stress tolerance were found among the 30 lines.The results suggest wide variations exist in alkali-salt stress tolerance among the 30 switchgrass lines.The approach of using a combination of principal components and cluster analysis of the physiological parameters and related ASTTI is feasible for evaluating alkali-salt tolerance in switchgrass.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang Province, P.R. China; Department of Crop and Soil Environmental Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America.

ABSTRACT
Soil salt-alkalization is a major limiting factor for crop production in many regions. Switchgrass (Panicum virgatum L.) is a warm-season C4 perennial rhizomatous bunchgrass and a target lignocellulosic biofuel species. The objective of this study was to evaluate relative alkali-salt tolerance among 30 switchgrass lines. Tillers of each switchgrass line were transplanted into pots filled with fine sand. Two months after transplanting, plants at E5 developmental stage were grown in either half strength Hoagland's nutrient solution with 0 mM Na+ (control) or half strength Hoagland's nutrient solution with 150 mM Na+ and pH of 9.5 (alkali-salt stress treatment) for 20 d. Alkali-salt stress damaged cell membranes [higher electrolyte leakage (EL)], reduced leaf relative water content (RWC), net photosynthetic rate (Pn), stomatal conductance (gs), and transpiration rate (Tr). An alkali-salt stress tolerance trait index (ASTTI) for each parameter was calculated based on the ratio of the value under alkali-salt stress and the value under non-stress conditions for each parameter of each line. Relative alkali-salt tolerance was determined based on principal components analysis and cluster analysis of the physiological parameters and their ASTTI values. Significant differences in alkali-salt stress tolerance were found among the 30 lines. Lowland lines TEM-SEC, Alamo, TEM-SLC and Kanlow were classified as alkali-salt tolerant. In contrast, three lowland lines (AM-314/MS-155, BN-13645-64) and two upland lines (Caddo and Blackwell-1) were classified as alkali-salt sensitive. The results suggest wide variations exist in alkali-salt stress tolerance among the 30 switchgrass lines. The approach of using a combination of principal components and cluster analysis of the physiological parameters and related ASTTI is feasible for evaluating alkali-salt tolerance in switchgrass.

No MeSH data available.


Related in: MedlinePlus

Stomatal conductance (gs) of 30 lines under control (solid bar) and alkali-salt stress (150 mM Na+ with pH of 9.5; open bar) at six days of alkali salt treatment.The line represents alkali-salt tolerance trait index (ASTTI). The greater the ASTTI, the better alkali salt tolerance. Values are means ±SE (n = 4). The bar represents LSD (0.05) for ASTTI.
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pone.0125305.g004: Stomatal conductance (gs) of 30 lines under control (solid bar) and alkali-salt stress (150 mM Na+ with pH of 9.5; open bar) at six days of alkali salt treatment.The line represents alkali-salt tolerance trait index (ASTTI). The greater the ASTTI, the better alkali salt tolerance. Values are means ±SE (n = 4). The bar represents LSD (0.05) for ASTTI.

Mentions: Alkali-salt stress reduced gs in all lines (Fig 4). The change of gs in response to alkali-salt stress varied greatly among the 30 lines. 70SG001, Grif Nebraska 28, TEM-SLC, Alamo, TEM-SEC, 70SG0023, and Kanlow had less gs reduction due to alkali-salt stress, with ASTTI values greater than 49.4%. 70SG001 had the greatest ASTTI of 84.0%. On the other hand, alkali-salt stress caused larger reductions in gs for 70SG0019, BN-8624-67, Summer, Blackwell-3, Pathfinder, TEM-LoDorm, and Caddo, which had ASTTI values less than 18.2%. 70SG0019 had the lowest gs, with an ASTTI value of 7.3%.


Physiological Evaluation of Alkali-Salt Tolerance of Thirty Switchgrass (Panicum virgatum) Lines.

Hu G, Liu Y, Zhang X, Yao F, Huang Y, Ervin EH, Zhao B - PLoS ONE (2015)

Stomatal conductance (gs) of 30 lines under control (solid bar) and alkali-salt stress (150 mM Na+ with pH of 9.5; open bar) at six days of alkali salt treatment.The line represents alkali-salt tolerance trait index (ASTTI). The greater the ASTTI, the better alkali salt tolerance. Values are means ±SE (n = 4). The bar represents LSD (0.05) for ASTTI.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492678&req=5

pone.0125305.g004: Stomatal conductance (gs) of 30 lines under control (solid bar) and alkali-salt stress (150 mM Na+ with pH of 9.5; open bar) at six days of alkali salt treatment.The line represents alkali-salt tolerance trait index (ASTTI). The greater the ASTTI, the better alkali salt tolerance. Values are means ±SE (n = 4). The bar represents LSD (0.05) for ASTTI.
Mentions: Alkali-salt stress reduced gs in all lines (Fig 4). The change of gs in response to alkali-salt stress varied greatly among the 30 lines. 70SG001, Grif Nebraska 28, TEM-SLC, Alamo, TEM-SEC, 70SG0023, and Kanlow had less gs reduction due to alkali-salt stress, with ASTTI values greater than 49.4%. 70SG001 had the greatest ASTTI of 84.0%. On the other hand, alkali-salt stress caused larger reductions in gs for 70SG0019, BN-8624-67, Summer, Blackwell-3, Pathfinder, TEM-LoDorm, and Caddo, which had ASTTI values less than 18.2%. 70SG0019 had the lowest gs, with an ASTTI value of 7.3%.

Bottom Line: Significant differences in alkali-salt stress tolerance were found among the 30 lines.The results suggest wide variations exist in alkali-salt stress tolerance among the 30 switchgrass lines.The approach of using a combination of principal components and cluster analysis of the physiological parameters and related ASTTI is feasible for evaluating alkali-salt tolerance in switchgrass.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang Province, P.R. China; Department of Crop and Soil Environmental Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America.

ABSTRACT
Soil salt-alkalization is a major limiting factor for crop production in many regions. Switchgrass (Panicum virgatum L.) is a warm-season C4 perennial rhizomatous bunchgrass and a target lignocellulosic biofuel species. The objective of this study was to evaluate relative alkali-salt tolerance among 30 switchgrass lines. Tillers of each switchgrass line were transplanted into pots filled with fine sand. Two months after transplanting, plants at E5 developmental stage were grown in either half strength Hoagland's nutrient solution with 0 mM Na+ (control) or half strength Hoagland's nutrient solution with 150 mM Na+ and pH of 9.5 (alkali-salt stress treatment) for 20 d. Alkali-salt stress damaged cell membranes [higher electrolyte leakage (EL)], reduced leaf relative water content (RWC), net photosynthetic rate (Pn), stomatal conductance (gs), and transpiration rate (Tr). An alkali-salt stress tolerance trait index (ASTTI) for each parameter was calculated based on the ratio of the value under alkali-salt stress and the value under non-stress conditions for each parameter of each line. Relative alkali-salt tolerance was determined based on principal components analysis and cluster analysis of the physiological parameters and their ASTTI values. Significant differences in alkali-salt stress tolerance were found among the 30 lines. Lowland lines TEM-SEC, Alamo, TEM-SLC and Kanlow were classified as alkali-salt tolerant. In contrast, three lowland lines (AM-314/MS-155, BN-13645-64) and two upland lines (Caddo and Blackwell-1) were classified as alkali-salt sensitive. The results suggest wide variations exist in alkali-salt stress tolerance among the 30 switchgrass lines. The approach of using a combination of principal components and cluster analysis of the physiological parameters and related ASTTI is feasible for evaluating alkali-salt tolerance in switchgrass.

No MeSH data available.


Related in: MedlinePlus