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Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer.

Ling XL, Zhang T, Hou XM, Zhao D - Onco Targets Ther (2015)

Bottom Line: Cox's Proportional Hazard Model was used to conduct a combined-effect analysis for each covariate.The results suggested that the high expression of fascin-1 was significantly correlated with lymph node metastasis (P=0.022) and TNM stage (P=0.042).Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with the overall survival in multivariate analyses by using the Cox regression model.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, The First Hospital of Lanzhou University (The Branch Hospital of Donggang), Lanzhou, Gansu Province, People's Republic of China.

ABSTRACT

Purpose: Fascin-1 promotes the formation of filopodia, lamellipodia, and microspikes of cell membrane after its cross-linking with F-actin, thereby enhancing the cell movement and metastasis and invasion of tumor cells. This study explored the fascin-1 protein's expression in non-small cell lung cancer (NSCLC) tissues and its relationship with clinical pathology and prognostic indicators.

Methods: Immunohistochemical analysis was used to determine the expression of fascin-1 in NSCLC tissues. We used quantitative real-time polymerase chain reaction and western blot analysis to further verify the results. The fascin-1 expression and statistical method for clinical pathological parameters are examined by χ (2). Kaplan-Meier method is used for survival analysis. Cox's Proportional Hazard Model was used to conduct a combined-effect analysis for each covariate.

Results: In 73 of the 128 cases, NSCLC cancer tissues (57.0%) were found with high expression of fascin-1, which was significantly higher than the adjacent tissues (35/128, 27.3%). The results suggested that the high expression of fascin-1 was significantly correlated with lymph node metastasis (P=0.022) and TNM stage (P=0.042). The high fascin-1 expression patients survived shorter than those NSCLC patients with low fascin-1 expression (P<0.05). Univariate analysis revealed that lymph node metastasis, TNM stage, and fascin-1 expression status were correlated with the overall survival. Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with the overall survival in multivariate analyses by using the Cox regression model.

Conclusion: The fascin-1 protein may be a useful prognostic indicator and hopeful new target for NSCLC patients.

No MeSH data available.


Related in: MedlinePlus

Kaplan–Meier curves of DFS and OS.Notes: (A) DFS of all patients with NSCLC, (B) OS of all patients with NSCLC, (C) OS according to lymph node metastasis in patients with NSCLC, and (D) OS according to stage in patients with NSCLC.Abbreviations: DFS, disease-free survival; OS, overall survival.
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f4-ott-8-1589: Kaplan–Meier curves of DFS and OS.Notes: (A) DFS of all patients with NSCLC, (B) OS of all patients with NSCLC, (C) OS according to lymph node metastasis in patients with NSCLC, and (D) OS according to stage in patients with NSCLC.Abbreviations: DFS, disease-free survival; OS, overall survival.

Mentions: Patients were divided into 2 groups according to the extent of fascin-1 expression (low vs high). Based on Kaplan–Meier curves, patients with high fascin-1 expression had shorter durations of survival than patients with low fascin-1 expression (P<0.05, Table 2 and Figure 4). A regression analysis was performed using the Cox’s proportional hazards model, which included all NSCLC clinical and pathological factors, as well as the fascin-1 expression status. Univariate analyses revealed that lymph node metastasis, TNM stage, and fascin-1 expression status were correlated with the OS. Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with OS in the multivariate analyses that employed the Cox regression model (Table 3). According to the results of this regression analysis, high expression of fascin-1 is an independent prognostic factor for shorter survival in patients with NSCLC.


Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer.

Ling XL, Zhang T, Hou XM, Zhao D - Onco Targets Ther (2015)

Kaplan–Meier curves of DFS and OS.Notes: (A) DFS of all patients with NSCLC, (B) OS of all patients with NSCLC, (C) OS according to lymph node metastasis in patients with NSCLC, and (D) OS according to stage in patients with NSCLC.Abbreviations: DFS, disease-free survival; OS, overall survival.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492659&req=5

f4-ott-8-1589: Kaplan–Meier curves of DFS and OS.Notes: (A) DFS of all patients with NSCLC, (B) OS of all patients with NSCLC, (C) OS according to lymph node metastasis in patients with NSCLC, and (D) OS according to stage in patients with NSCLC.Abbreviations: DFS, disease-free survival; OS, overall survival.
Mentions: Patients were divided into 2 groups according to the extent of fascin-1 expression (low vs high). Based on Kaplan–Meier curves, patients with high fascin-1 expression had shorter durations of survival than patients with low fascin-1 expression (P<0.05, Table 2 and Figure 4). A regression analysis was performed using the Cox’s proportional hazards model, which included all NSCLC clinical and pathological factors, as well as the fascin-1 expression status. Univariate analyses revealed that lymph node metastasis, TNM stage, and fascin-1 expression status were correlated with the OS. Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with OS in the multivariate analyses that employed the Cox regression model (Table 3). According to the results of this regression analysis, high expression of fascin-1 is an independent prognostic factor for shorter survival in patients with NSCLC.

Bottom Line: Cox's Proportional Hazard Model was used to conduct a combined-effect analysis for each covariate.The results suggested that the high expression of fascin-1 was significantly correlated with lymph node metastasis (P=0.022) and TNM stage (P=0.042).Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with the overall survival in multivariate analyses by using the Cox regression model.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, The First Hospital of Lanzhou University (The Branch Hospital of Donggang), Lanzhou, Gansu Province, People's Republic of China.

ABSTRACT

Purpose: Fascin-1 promotes the formation of filopodia, lamellipodia, and microspikes of cell membrane after its cross-linking with F-actin, thereby enhancing the cell movement and metastasis and invasion of tumor cells. This study explored the fascin-1 protein's expression in non-small cell lung cancer (NSCLC) tissues and its relationship with clinical pathology and prognostic indicators.

Methods: Immunohistochemical analysis was used to determine the expression of fascin-1 in NSCLC tissues. We used quantitative real-time polymerase chain reaction and western blot analysis to further verify the results. The fascin-1 expression and statistical method for clinical pathological parameters are examined by χ (2). Kaplan-Meier method is used for survival analysis. Cox's Proportional Hazard Model was used to conduct a combined-effect analysis for each covariate.

Results: In 73 of the 128 cases, NSCLC cancer tissues (57.0%) were found with high expression of fascin-1, which was significantly higher than the adjacent tissues (35/128, 27.3%). The results suggested that the high expression of fascin-1 was significantly correlated with lymph node metastasis (P=0.022) and TNM stage (P=0.042). The high fascin-1 expression patients survived shorter than those NSCLC patients with low fascin-1 expression (P<0.05). Univariate analysis revealed that lymph node metastasis, TNM stage, and fascin-1 expression status were correlated with the overall survival. Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with the overall survival in multivariate analyses by using the Cox regression model.

Conclusion: The fascin-1 protein may be a useful prognostic indicator and hopeful new target for NSCLC patients.

No MeSH data available.


Related in: MedlinePlus