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Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer.

Ling XL, Zhang T, Hou XM, Zhao D - Onco Targets Ther (2015)

Bottom Line: Cox's Proportional Hazard Model was used to conduct a combined-effect analysis for each covariate.The results suggested that the high expression of fascin-1 was significantly correlated with lymph node metastasis (P=0.022) and TNM stage (P=0.042).Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with the overall survival in multivariate analyses by using the Cox regression model.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, The First Hospital of Lanzhou University (The Branch Hospital of Donggang), Lanzhou, Gansu Province, People's Republic of China.

ABSTRACT

Purpose: Fascin-1 promotes the formation of filopodia, lamellipodia, and microspikes of cell membrane after its cross-linking with F-actin, thereby enhancing the cell movement and metastasis and invasion of tumor cells. This study explored the fascin-1 protein's expression in non-small cell lung cancer (NSCLC) tissues and its relationship with clinical pathology and prognostic indicators.

Methods: Immunohistochemical analysis was used to determine the expression of fascin-1 in NSCLC tissues. We used quantitative real-time polymerase chain reaction and western blot analysis to further verify the results. The fascin-1 expression and statistical method for clinical pathological parameters are examined by χ (2). Kaplan-Meier method is used for survival analysis. Cox's Proportional Hazard Model was used to conduct a combined-effect analysis for each covariate.

Results: In 73 of the 128 cases, NSCLC cancer tissues (57.0%) were found with high expression of fascin-1, which was significantly higher than the adjacent tissues (35/128, 27.3%). The results suggested that the high expression of fascin-1 was significantly correlated with lymph node metastasis (P=0.022) and TNM stage (P=0.042). The high fascin-1 expression patients survived shorter than those NSCLC patients with low fascin-1 expression (P<0.05). Univariate analysis revealed that lymph node metastasis, TNM stage, and fascin-1 expression status were correlated with the overall survival. Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with the overall survival in multivariate analyses by using the Cox regression model.

Conclusion: The fascin-1 protein may be a useful prognostic indicator and hopeful new target for NSCLC patients.

No MeSH data available.


Related in: MedlinePlus

Fascin-1 protein was detected by western blot analysis.Abbreviation: NSCLC, non-small cell lung cancer.
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f3-ott-8-1589: Fascin-1 protein was detected by western blot analysis.Abbreviation: NSCLC, non-small cell lung cancer.

Mentions: Fascin-1 expression was detected in 128 primary NSCLC and adjacent tissues by immunohistochemistry. Immunohistochemical results showed that fascin-1 was mainly localized in the in the nucleus and cytoplasm of tumor (Figure 1). The results suggest fascin-1 have different levels of expression in NSCLC and adjacent tissues. In accordance with the evaluation criteria described previously, we found that in 73 of 128 cases, NSCLC cancer tissues (57.0%) had high expression of fascin-1, which significantly higher than the adjacent tissues (35/128, 27.3%). In order to further verify this result, we observed the expression levels of fascin-1 mRNA and protein using reverse-transcription polymerase chain reaction and western blot. The result indicated that the expression of fascin-1 was significantly higher in NSCLC tissues than that in adjacent tissues (P<0.01), (Figures 2 and 3).


Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer.

Ling XL, Zhang T, Hou XM, Zhao D - Onco Targets Ther (2015)

Fascin-1 protein was detected by western blot analysis.Abbreviation: NSCLC, non-small cell lung cancer.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492659&req=5

f3-ott-8-1589: Fascin-1 protein was detected by western blot analysis.Abbreviation: NSCLC, non-small cell lung cancer.
Mentions: Fascin-1 expression was detected in 128 primary NSCLC and adjacent tissues by immunohistochemistry. Immunohistochemical results showed that fascin-1 was mainly localized in the in the nucleus and cytoplasm of tumor (Figure 1). The results suggest fascin-1 have different levels of expression in NSCLC and adjacent tissues. In accordance with the evaluation criteria described previously, we found that in 73 of 128 cases, NSCLC cancer tissues (57.0%) had high expression of fascin-1, which significantly higher than the adjacent tissues (35/128, 27.3%). In order to further verify this result, we observed the expression levels of fascin-1 mRNA and protein using reverse-transcription polymerase chain reaction and western blot. The result indicated that the expression of fascin-1 was significantly higher in NSCLC tissues than that in adjacent tissues (P<0.01), (Figures 2 and 3).

Bottom Line: Cox's Proportional Hazard Model was used to conduct a combined-effect analysis for each covariate.The results suggested that the high expression of fascin-1 was significantly correlated with lymph node metastasis (P=0.022) and TNM stage (P=0.042).Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with the overall survival in multivariate analyses by using the Cox regression model.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, The First Hospital of Lanzhou University (The Branch Hospital of Donggang), Lanzhou, Gansu Province, People's Republic of China.

ABSTRACT

Purpose: Fascin-1 promotes the formation of filopodia, lamellipodia, and microspikes of cell membrane after its cross-linking with F-actin, thereby enhancing the cell movement and metastasis and invasion of tumor cells. This study explored the fascin-1 protein's expression in non-small cell lung cancer (NSCLC) tissues and its relationship with clinical pathology and prognostic indicators.

Methods: Immunohistochemical analysis was used to determine the expression of fascin-1 in NSCLC tissues. We used quantitative real-time polymerase chain reaction and western blot analysis to further verify the results. The fascin-1 expression and statistical method for clinical pathological parameters are examined by χ (2). Kaplan-Meier method is used for survival analysis. Cox's Proportional Hazard Model was used to conduct a combined-effect analysis for each covariate.

Results: In 73 of the 128 cases, NSCLC cancer tissues (57.0%) were found with high expression of fascin-1, which was significantly higher than the adjacent tissues (35/128, 27.3%). The results suggested that the high expression of fascin-1 was significantly correlated with lymph node metastasis (P=0.022) and TNM stage (P=0.042). The high fascin-1 expression patients survived shorter than those NSCLC patients with low fascin-1 expression (P<0.05). Univariate analysis revealed that lymph node metastasis, TNM stage, and fascin-1 expression status were correlated with the overall survival. Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with the overall survival in multivariate analyses by using the Cox regression model.

Conclusion: The fascin-1 protein may be a useful prognostic indicator and hopeful new target for NSCLC patients.

No MeSH data available.


Related in: MedlinePlus