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Biofumigation on Post-Harvest Diseases of Fruits Using a New Volatile-Producing Fungus of Ceratocystis fimbriata.

Li Q, Wu L, Hao J, Luo L, Cao Y, Li J - PLoS ONE (2015)

Bottom Line: Two post-harvest diseases, peach brown rot caused by Monilinia fructicola and citrus green mold caused by Penicillium digitatum, were controlled during a 4-day storage by enclosing wound-inoculated fruits with 10 standard diameter Petri plate cultures of C. fimbriata in a 15 L box.The fruits were freshly inoculated at onset of storage and the cultures of C. fimbriata were 6 days old.Percentage of control was 92 and 97%, respectively.

View Article: PubMed Central - PubMed

Affiliation: Beijing Engineering Research Center of Seed and Plant Health (BERC-SPH) / Beijing Key Laboratory of Seed Disease Testing and Control (BKL-SDTC), Beijing, P. R. China.

ABSTRACT
A variety of volatile organic compounds (VOCs) produced by Ceratocystis fimbriata have strong bioactivity against a wide range of fungi, bacteria and oomycetes. Mycelial growth, conidial production, and spore germination of fungi and oomycetes were significantly inhibited after exposure to cultures of C. fimbriata, and colony formation of bacteria was also inhibited. Two post-harvest diseases, peach brown rot caused by Monilinia fructicola and citrus green mold caused by Penicillium digitatum, were controlled during a 4-day storage by enclosing wound-inoculated fruits with 10 standard diameter Petri plate cultures of C. fimbriata in a 15 L box. The fruits were freshly inoculated at onset of storage and the cultures of C. fimbriata were 6 days old. Percentage of control was 92 and 97%, respectively. After exposure to C. fimbriata VOCs, severely misshapen hyphae and conidia of these two post-harvest pathogens were observed by scanning electron microscopy, and their pathogenicity was lost or greatly reduced.

No MeSH data available.


Related in: MedlinePlus

Effect of the treatment by the VOCs from Ceratocystis fimbriata applied immediately, 1 or 2 days after inoculation on the lesion size (mm2) and control effect (%) of peach brown rot and citrus green mold for four days.Error bars represent the standard deviation of ten replicates.
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pone.0132009.g003: Effect of the treatment by the VOCs from Ceratocystis fimbriata applied immediately, 1 or 2 days after inoculation on the lesion size (mm2) and control effect (%) of peach brown rot and citrus green mold for four days.Error bars represent the standard deviation of ten replicates.

Mentions: In the treatment without C. fimbriata volatiles (negative control), the fruits were 100% infected by M. fructicola or P. digitatum and visible rot or mold was observed on wound locations inoculated with conidial suspension (Fig 2C). In the treatments with C. fimbriata volatiles, no visible symptoms were observed on the fruits whether they were inoculated with conidial suspension or sterile water (Fig 2D and 2E). The disease control effect on peach brown rot and citrus green mold was 94% and 91% respectively when the VOCs treatment was performed immediately after inoculation with the conidia suspension, which was the optimum application time for the treatment; when treated 24 hours post inoculation (HPI) disease control sharply decreased to 35% and 34%, respectively. Disease control was only 15% and 9% respectively when treated 48 HPI for the two diseases (Fig 3).


Biofumigation on Post-Harvest Diseases of Fruits Using a New Volatile-Producing Fungus of Ceratocystis fimbriata.

Li Q, Wu L, Hao J, Luo L, Cao Y, Li J - PLoS ONE (2015)

Effect of the treatment by the VOCs from Ceratocystis fimbriata applied immediately, 1 or 2 days after inoculation on the lesion size (mm2) and control effect (%) of peach brown rot and citrus green mold for four days.Error bars represent the standard deviation of ten replicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492557&req=5

pone.0132009.g003: Effect of the treatment by the VOCs from Ceratocystis fimbriata applied immediately, 1 or 2 days after inoculation on the lesion size (mm2) and control effect (%) of peach brown rot and citrus green mold for four days.Error bars represent the standard deviation of ten replicates.
Mentions: In the treatment without C. fimbriata volatiles (negative control), the fruits were 100% infected by M. fructicola or P. digitatum and visible rot or mold was observed on wound locations inoculated with conidial suspension (Fig 2C). In the treatments with C. fimbriata volatiles, no visible symptoms were observed on the fruits whether they were inoculated with conidial suspension or sterile water (Fig 2D and 2E). The disease control effect on peach brown rot and citrus green mold was 94% and 91% respectively when the VOCs treatment was performed immediately after inoculation with the conidia suspension, which was the optimum application time for the treatment; when treated 24 hours post inoculation (HPI) disease control sharply decreased to 35% and 34%, respectively. Disease control was only 15% and 9% respectively when treated 48 HPI for the two diseases (Fig 3).

Bottom Line: Two post-harvest diseases, peach brown rot caused by Monilinia fructicola and citrus green mold caused by Penicillium digitatum, were controlled during a 4-day storage by enclosing wound-inoculated fruits with 10 standard diameter Petri plate cultures of C. fimbriata in a 15 L box.The fruits were freshly inoculated at onset of storage and the cultures of C. fimbriata were 6 days old.Percentage of control was 92 and 97%, respectively.

View Article: PubMed Central - PubMed

Affiliation: Beijing Engineering Research Center of Seed and Plant Health (BERC-SPH) / Beijing Key Laboratory of Seed Disease Testing and Control (BKL-SDTC), Beijing, P. R. China.

ABSTRACT
A variety of volatile organic compounds (VOCs) produced by Ceratocystis fimbriata have strong bioactivity against a wide range of fungi, bacteria and oomycetes. Mycelial growth, conidial production, and spore germination of fungi and oomycetes were significantly inhibited after exposure to cultures of C. fimbriata, and colony formation of bacteria was also inhibited. Two post-harvest diseases, peach brown rot caused by Monilinia fructicola and citrus green mold caused by Penicillium digitatum, were controlled during a 4-day storage by enclosing wound-inoculated fruits with 10 standard diameter Petri plate cultures of C. fimbriata in a 15 L box. The fruits were freshly inoculated at onset of storage and the cultures of C. fimbriata were 6 days old. Percentage of control was 92 and 97%, respectively. After exposure to C. fimbriata VOCs, severely misshapen hyphae and conidia of these two post-harvest pathogens were observed by scanning electron microscopy, and their pathogenicity was lost or greatly reduced.

No MeSH data available.


Related in: MedlinePlus