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A 1H-NMR Based Study on Hemolymph Metabolomics in Eri Silkworm after Oral Administration of 1-Deoxynojirimycin.

Deng MJ, Lin XD, Lin QT, Wen DF, Zhang ML, Wang XQ, Gao HC, Xu JP - PLoS ONE (2015)

Bottom Line: Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test.All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism.DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, China; Analytical and Testing Center of Wenzhou Medical University, Wenzhou, China.

ABSTRACT
We aimed to investigate whether 1-deoxynojirimycin (DNJ) modulates glycometabolism and has toxicity in Eri silkworm (Samia cynthia ricini, Saturniidae). In this paper, hemolymph metabolites were used to explore metabolic changes after oral administration of DNJ or mulberry latex and to characterize the biological function of DNJ at the metabolic and systemic levels. Hemolymph samples were collected from fourth-instar larvae of Eri silkworm and ex-vivo high-resolution 1H nuclear magnetic resonance (NMR) spectra were acquired from the collected hemolymph samples. Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test. Metabolic pattern recognition analysis of hemolymph samples indicated that the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) were significantly different from the control group. Moreover, compared to the control group, the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) showed the decreased levels of citrate, succinate, fumarate, malate, and glutamine in hemolymph, the groups of 0.25% DNJ and the mixture of 0.5% DNJ and latex (1:1) showed the increased levels of trehalose and lactate. In addition, mulberry leaves exude latex was highly toxic to Eri silkworm because rich unidentified high-molecular-weight factor (s) acted as toxic substances. In our results, latex caused 20 deaths among 50 fourth-instar larvae of Eri silkmoth, but DNJ or the mixture did not caused death. All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism. DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

No MeSH data available.


Related in: MedlinePlus

Schematic diagram of the metabolic pathways.The metabolite changes detected by 1H NMR hemolymph analysis and the pathway referenced to the KEGG database show the interrelationship of the identified metabolic pathways involved in the experimental Eri silkworms. Metabolites with increased and decreased levels compared to control silkworms are indicated in red and blue.
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pone.0131696.g005: Schematic diagram of the metabolic pathways.The metabolite changes detected by 1H NMR hemolymph analysis and the pathway referenced to the KEGG database show the interrelationship of the identified metabolic pathways involved in the experimental Eri silkworms. Metabolites with increased and decreased levels compared to control silkworms are indicated in red and blue.

Mentions: As an important class of glycosidase suppressants, iminosugars recently became the study focus of potential therapeutic agents [27–29]. As one kind of iminosugars, DNJ shows the high in-vitro inhibition activity on α-glucosidase. Its in-vivo efficacy and mechanism are obscure, especially in caterpillars. In our study, the 1H NMR spectra of hemolymph samples of Eri silkworms after different preconditioning treatments revealed the significant changes in the concentrations of critical endogenous metabolites compared to those in the control group. The global metabolic abnormalities related to specific metabolic pathways, such as glycolysis, gluconeogenesis, TCA cycle, and energy and amino acid metabolism, were determined. The results expedite us to explore the metabolism mechanisms of DNJ. Fig 5 illustrates the altered metabolic pathways in hemolymph of Eri silkworms after oral administration of 0.25% DNJ, latex and mixture based on the KEGG database (http://www.genome.jp/kegg/pathway.html).


A 1H-NMR Based Study on Hemolymph Metabolomics in Eri Silkworm after Oral Administration of 1-Deoxynojirimycin.

Deng MJ, Lin XD, Lin QT, Wen DF, Zhang ML, Wang XQ, Gao HC, Xu JP - PLoS ONE (2015)

Schematic diagram of the metabolic pathways.The metabolite changes detected by 1H NMR hemolymph analysis and the pathway referenced to the KEGG database show the interrelationship of the identified metabolic pathways involved in the experimental Eri silkworms. Metabolites with increased and decreased levels compared to control silkworms are indicated in red and blue.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492494&req=5

pone.0131696.g005: Schematic diagram of the metabolic pathways.The metabolite changes detected by 1H NMR hemolymph analysis and the pathway referenced to the KEGG database show the interrelationship of the identified metabolic pathways involved in the experimental Eri silkworms. Metabolites with increased and decreased levels compared to control silkworms are indicated in red and blue.
Mentions: As an important class of glycosidase suppressants, iminosugars recently became the study focus of potential therapeutic agents [27–29]. As one kind of iminosugars, DNJ shows the high in-vitro inhibition activity on α-glucosidase. Its in-vivo efficacy and mechanism are obscure, especially in caterpillars. In our study, the 1H NMR spectra of hemolymph samples of Eri silkworms after different preconditioning treatments revealed the significant changes in the concentrations of critical endogenous metabolites compared to those in the control group. The global metabolic abnormalities related to specific metabolic pathways, such as glycolysis, gluconeogenesis, TCA cycle, and energy and amino acid metabolism, were determined. The results expedite us to explore the metabolism mechanisms of DNJ. Fig 5 illustrates the altered metabolic pathways in hemolymph of Eri silkworms after oral administration of 0.25% DNJ, latex and mixture based on the KEGG database (http://www.genome.jp/kegg/pathway.html).

Bottom Line: Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test.All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism.DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, China; Analytical and Testing Center of Wenzhou Medical University, Wenzhou, China.

ABSTRACT
We aimed to investigate whether 1-deoxynojirimycin (DNJ) modulates glycometabolism and has toxicity in Eri silkworm (Samia cynthia ricini, Saturniidae). In this paper, hemolymph metabolites were used to explore metabolic changes after oral administration of DNJ or mulberry latex and to characterize the biological function of DNJ at the metabolic and systemic levels. Hemolymph samples were collected from fourth-instar larvae of Eri silkworm and ex-vivo high-resolution 1H nuclear magnetic resonance (NMR) spectra were acquired from the collected hemolymph samples. Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test. Metabolic pattern recognition analysis of hemolymph samples indicated that the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) were significantly different from the control group. Moreover, compared to the control group, the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) showed the decreased levels of citrate, succinate, fumarate, malate, and glutamine in hemolymph, the groups of 0.25% DNJ and the mixture of 0.5% DNJ and latex (1:1) showed the increased levels of trehalose and lactate. In addition, mulberry leaves exude latex was highly toxic to Eri silkworm because rich unidentified high-molecular-weight factor (s) acted as toxic substances. In our results, latex caused 20 deaths among 50 fourth-instar larvae of Eri silkmoth, but DNJ or the mixture did not caused death. All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism. DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

No MeSH data available.


Related in: MedlinePlus