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A 1H-NMR Based Study on Hemolymph Metabolomics in Eri Silkworm after Oral Administration of 1-Deoxynojirimycin.

Deng MJ, Lin XD, Lin QT, Wen DF, Zhang ML, Wang XQ, Gao HC, Xu JP - PLoS ONE (2015)

Bottom Line: Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test.All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism.DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, China; Analytical and Testing Center of Wenzhou Medical University, Wenzhou, China.

ABSTRACT
We aimed to investigate whether 1-deoxynojirimycin (DNJ) modulates glycometabolism and has toxicity in Eri silkworm (Samia cynthia ricini, Saturniidae). In this paper, hemolymph metabolites were used to explore metabolic changes after oral administration of DNJ or mulberry latex and to characterize the biological function of DNJ at the metabolic and systemic levels. Hemolymph samples were collected from fourth-instar larvae of Eri silkworm and ex-vivo high-resolution 1H nuclear magnetic resonance (NMR) spectra were acquired from the collected hemolymph samples. Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test. Metabolic pattern recognition analysis of hemolymph samples indicated that the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) were significantly different from the control group. Moreover, compared to the control group, the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) showed the decreased levels of citrate, succinate, fumarate, malate, and glutamine in hemolymph, the groups of 0.25% DNJ and the mixture of 0.5% DNJ and latex (1:1) showed the increased levels of trehalose and lactate. In addition, mulberry leaves exude latex was highly toxic to Eri silkworm because rich unidentified high-molecular-weight factor (s) acted as toxic substances. In our results, latex caused 20 deaths among 50 fourth-instar larvae of Eri silkmoth, but DNJ or the mixture did not caused death. All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism. DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

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Related in: MedlinePlus

Metabolic changes found in 1H NMR spectra of hemolymph samples of the control group, 0.25% DNJ group, latex group, and the mixture group, respectively.Values are expressed as mean±SD. * and ** mean the significant (P<0.05) and extremely significant differences (P<0.01) compared to the control group.
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pone.0131696.g004: Metabolic changes found in 1H NMR spectra of hemolymph samples of the control group, 0.25% DNJ group, latex group, and the mixture group, respectively.Values are expressed as mean±SD. * and ** mean the significant (P<0.05) and extremely significant differences (P<0.01) compared to the control group.

Mentions: According to the PCA score plots obtained from the hemolymph of latex, mixture and the control groups, three groups showed significant differences along the PC1 direction and PC2 direction (Fig 3C, R2 = 0.858, Q2 = 0.636). The significant differences between the control group and two treatment groups (latex group and the mixture group) indicate that metabolic turbulence occurs in Eri silkworms of the latex and mixture groups. The corresponding loading plot (Fig 3D) shows that the variables related to succinate, o-phosphocholine, glycine, trehalose and other metabolites are mainly responsible for the differentiation (Fig 4).


A 1H-NMR Based Study on Hemolymph Metabolomics in Eri Silkworm after Oral Administration of 1-Deoxynojirimycin.

Deng MJ, Lin XD, Lin QT, Wen DF, Zhang ML, Wang XQ, Gao HC, Xu JP - PLoS ONE (2015)

Metabolic changes found in 1H NMR spectra of hemolymph samples of the control group, 0.25% DNJ group, latex group, and the mixture group, respectively.Values are expressed as mean±SD. * and ** mean the significant (P<0.05) and extremely significant differences (P<0.01) compared to the control group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492494&req=5

pone.0131696.g004: Metabolic changes found in 1H NMR spectra of hemolymph samples of the control group, 0.25% DNJ group, latex group, and the mixture group, respectively.Values are expressed as mean±SD. * and ** mean the significant (P<0.05) and extremely significant differences (P<0.01) compared to the control group.
Mentions: According to the PCA score plots obtained from the hemolymph of latex, mixture and the control groups, three groups showed significant differences along the PC1 direction and PC2 direction (Fig 3C, R2 = 0.858, Q2 = 0.636). The significant differences between the control group and two treatment groups (latex group and the mixture group) indicate that metabolic turbulence occurs in Eri silkworms of the latex and mixture groups. The corresponding loading plot (Fig 3D) shows that the variables related to succinate, o-phosphocholine, glycine, trehalose and other metabolites are mainly responsible for the differentiation (Fig 4).

Bottom Line: Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test.All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism.DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, China; Analytical and Testing Center of Wenzhou Medical University, Wenzhou, China.

ABSTRACT
We aimed to investigate whether 1-deoxynojirimycin (DNJ) modulates glycometabolism and has toxicity in Eri silkworm (Samia cynthia ricini, Saturniidae). In this paper, hemolymph metabolites were used to explore metabolic changes after oral administration of DNJ or mulberry latex and to characterize the biological function of DNJ at the metabolic and systemic levels. Hemolymph samples were collected from fourth-instar larvae of Eri silkworm and ex-vivo high-resolution 1H nuclear magnetic resonance (NMR) spectra were acquired from the collected hemolymph samples. Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test. Metabolic pattern recognition analysis of hemolymph samples indicated that the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) were significantly different from the control group. Moreover, compared to the control group, the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) showed the decreased levels of citrate, succinate, fumarate, malate, and glutamine in hemolymph, the groups of 0.25% DNJ and the mixture of 0.5% DNJ and latex (1:1) showed the increased levels of trehalose and lactate. In addition, mulberry leaves exude latex was highly toxic to Eri silkworm because rich unidentified high-molecular-weight factor (s) acted as toxic substances. In our results, latex caused 20 deaths among 50 fourth-instar larvae of Eri silkmoth, but DNJ or the mixture did not caused death. All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism. DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

No MeSH data available.


Related in: MedlinePlus