Limits...
A 1H-NMR Based Study on Hemolymph Metabolomics in Eri Silkworm after Oral Administration of 1-Deoxynojirimycin.

Deng MJ, Lin XD, Lin QT, Wen DF, Zhang ML, Wang XQ, Gao HC, Xu JP - PLoS ONE (2015)

Bottom Line: Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test.All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism.DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, China; Analytical and Testing Center of Wenzhou Medical University, Wenzhou, China.

ABSTRACT
We aimed to investigate whether 1-deoxynojirimycin (DNJ) modulates glycometabolism and has toxicity in Eri silkworm (Samia cynthia ricini, Saturniidae). In this paper, hemolymph metabolites were used to explore metabolic changes after oral administration of DNJ or mulberry latex and to characterize the biological function of DNJ at the metabolic and systemic levels. Hemolymph samples were collected from fourth-instar larvae of Eri silkworm and ex-vivo high-resolution 1H nuclear magnetic resonance (NMR) spectra were acquired from the collected hemolymph samples. Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test. Metabolic pattern recognition analysis of hemolymph samples indicated that the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) were significantly different from the control group. Moreover, compared to the control group, the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) showed the decreased levels of citrate, succinate, fumarate, malate, and glutamine in hemolymph, the groups of 0.25% DNJ and the mixture of 0.5% DNJ and latex (1:1) showed the increased levels of trehalose and lactate. In addition, mulberry leaves exude latex was highly toxic to Eri silkworm because rich unidentified high-molecular-weight factor (s) acted as toxic substances. In our results, latex caused 20 deaths among 50 fourth-instar larvae of Eri silkmoth, but DNJ or the mixture did not caused death. All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism. DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

No MeSH data available.


Related in: MedlinePlus

Typical 1H NMR spectra of hemolymph of Eri silkworms of four groups: control group (A), 0.25% DNJ group (B), latex group (C), and the mixture group (D).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4492494&req=5

pone.0131696.g002: Typical 1H NMR spectra of hemolymph of Eri silkworms of four groups: control group (A), 0.25% DNJ group (B), latex group (C), and the mixture group (D).

Mentions: In the typical 1H-NMR spectra of hemolymph samples from Eri silkworms in four groups (Fig 2A–2D), the spectrographic resonances of the metabolites were designated based on our published work [19,24–26] as well as the 600-MHz library in Chenomx NMR Suite 7.0 (Chenomx Inc., Edmonton, Canada). In addition, partial samples were checked to identify the assignments based on 1D 1H NMR spectra according to 2D 1H-1H COSY spectra obtained under solvent suppression. Based on the 1H-NMR spectra of the hemolymph, we simultaneously detected several endogenous metabolites, including leucine (δ0.95), valine (δ1.04), lactate (δ1.32), alanine (δ1.48), lysine (δ1.72), glutamine (δ2.13), succinate (δ2.40), citrate (δ2.54), malate (δ2.66), glycine (δ3.56), o-phosphocholine (δ4.16), threonine (δ4.25), trigonelline (δ4.44), trehalose (δ5.19), fumarate (δ6.52), tyrosine (δ7.19), and histidine (δ7.86), (S1 Table).


A 1H-NMR Based Study on Hemolymph Metabolomics in Eri Silkworm after Oral Administration of 1-Deoxynojirimycin.

Deng MJ, Lin XD, Lin QT, Wen DF, Zhang ML, Wang XQ, Gao HC, Xu JP - PLoS ONE (2015)

Typical 1H NMR spectra of hemolymph of Eri silkworms of four groups: control group (A), 0.25% DNJ group (B), latex group (C), and the mixture group (D).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492494&req=5

pone.0131696.g002: Typical 1H NMR spectra of hemolymph of Eri silkworms of four groups: control group (A), 0.25% DNJ group (B), latex group (C), and the mixture group (D).
Mentions: In the typical 1H-NMR spectra of hemolymph samples from Eri silkworms in four groups (Fig 2A–2D), the spectrographic resonances of the metabolites were designated based on our published work [19,24–26] as well as the 600-MHz library in Chenomx NMR Suite 7.0 (Chenomx Inc., Edmonton, Canada). In addition, partial samples were checked to identify the assignments based on 1D 1H NMR spectra according to 2D 1H-1H COSY spectra obtained under solvent suppression. Based on the 1H-NMR spectra of the hemolymph, we simultaneously detected several endogenous metabolites, including leucine (δ0.95), valine (δ1.04), lactate (δ1.32), alanine (δ1.48), lysine (δ1.72), glutamine (δ2.13), succinate (δ2.40), citrate (δ2.54), malate (δ2.66), glycine (δ3.56), o-phosphocholine (δ4.16), threonine (δ4.25), trigonelline (δ4.44), trehalose (δ5.19), fumarate (δ6.52), tyrosine (δ7.19), and histidine (δ7.86), (S1 Table).

Bottom Line: Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test.All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism.DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences, Anhui Agricultural University, Hefei, China; Analytical and Testing Center of Wenzhou Medical University, Wenzhou, China.

ABSTRACT
We aimed to investigate whether 1-deoxynojirimycin (DNJ) modulates glycometabolism and has toxicity in Eri silkworm (Samia cynthia ricini, Saturniidae). In this paper, hemolymph metabolites were used to explore metabolic changes after oral administration of DNJ or mulberry latex and to characterize the biological function of DNJ at the metabolic and systemic levels. Hemolymph samples were collected from fourth-instar larvae of Eri silkworm and ex-vivo high-resolution 1H nuclear magnetic resonance (NMR) spectra were acquired from the collected hemolymph samples. Then the obtained spectra were analyzed by principal component analysis (PCA) and independent-samples t-test. Metabolic pattern recognition analysis of hemolymph samples indicated that the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) were significantly different from the control group. Moreover, compared to the control group, the groups of 0.25% DNJ, latex, and the mixture of 0.5% DNJ and latex (1:1) showed the decreased levels of citrate, succinate, fumarate, malate, and glutamine in hemolymph, the groups of 0.25% DNJ and the mixture of 0.5% DNJ and latex (1:1) showed the increased levels of trehalose and lactate. In addition, mulberry leaves exude latex was highly toxic to Eri silkworm because rich unidentified high-molecular-weight factor (s) acted as toxic substances. In our results, latex caused 20 deaths among 50 fourth-instar larvae of Eri silkmoth, but DNJ or the mixture did not caused death. All these results suggest that DNJ has a positive impact on the reverse glycometabolism by modulating glycometabolism and inhibiting glucogenesis and energy metabolism. DNJ is a secure substance as a single-ingredient antidiabetic medicine due to its nontoxicity and bioactivity.

No MeSH data available.


Related in: MedlinePlus