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Platelets from Asthmatic Individuals Show Less Reliance on Glycolysis.

Xu W, Cardenes N, Corey C, Erzurum SC, Shiva S - PLoS ONE (2015)

Bottom Line: Further, several studies demonstrate altered mitochondrial function in asthmatic airways and suggest that these changes may be systemic.However, it is unknown whether systemic metabolic changes can be detected in circulating cells in asthmatic patients.The implications for this potential metabolic shift will be discussed in the context of increased oxidative stress and hypoxic adaptation of asthmatic patients.

View Article: PubMed Central - PubMed

Affiliation: Lerner Research Institute, Cleveland, Ohio, United States of America.

ABSTRACT
Asthma, a chronic inflammatory airway disease, is typified by high levels of TH2-cytokines and excessive generation of reactive nitrogen and oxygen species, which contribute to bronchial epithelial injury and airway remodeling. While immune function plays a major role in the pathogenesis of the disease, accumulating evidence suggests that altered cellular metabolism is a key determinant in the predisposition and disease progression of asthma. Further, several studies demonstrate altered mitochondrial function in asthmatic airways and suggest that these changes may be systemic. However, it is unknown whether systemic metabolic changes can be detected in circulating cells in asthmatic patients. Platelets are easily accessible blood cells that are known to propagate airway inflammation in asthma. Here we perform a bioenergetic screen of platelets from asthmatic and healthy individuals and demonstrate that asthmatic platelets show a decreased reliance on glycolytic processes and have increased tricarboxylic acid cycle activity. These data demonstrate a systemic alteration in asthma and are consistent with prior reports suggesting that oxidative phosphorylation is more efficient asthmatic individuals. The implications for this potential metabolic shift will be discussed in the context of increased oxidative stress and hypoxic adaptation of asthmatic patients. Further, these data suggest that platelets are potentially a good model for the monitoring of bioenergetic changes in asthma.

No MeSH data available.


Related in: MedlinePlus

Asthmatics show airflow obstruction compared to healthy controls.Airflow obstruction as measured by (A) FEV1% predicted and (B) FEV1/FVC in asthmatic individuals compared with healthy control subjects. #p<0.05.
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pone.0132007.g001: Asthmatics show airflow obstruction compared to healthy controls.Airflow obstruction as measured by (A) FEV1% predicted and (B) FEV1/FVC in asthmatic individuals compared with healthy control subjects. #p<0.05.

Mentions: To investigate platelet metabolism in asthma, a study population including 13 healthy controls and 12 individuals with asthma was utilized (Table 1). The asthmatics had airway reactivity to methacholine, but all asthmatic subjects were non-severe, as defined by criteria of the American Thoracic Society [39]. This was demonstrated by assessment of airflow by measurement of forced expiratory volume in 1 second (FEV1)% predicted and the ratio of FEV1 to forced vital capacity (FVC) (Fig 1). These data demonstrate that although asthmatics had significantly lower airflow compared to control subjects, the airflow limitation was generally mild to moderate [FEV1% predicted: 99 ± 3 (controls) vs 81 ± 5 (asthma), P = 0.01; FEV1/FVC: 0.82 ± 0.02 (controls) vs 0.73 ± 0.03 (asthma), P = 0.03]. Asthmatics were well controlled for at least the prior 6 weeks or longer, and withheld medications prior to day of testing. The number of subjects assessed for each experiment is provided with each result.


Platelets from Asthmatic Individuals Show Less Reliance on Glycolysis.

Xu W, Cardenes N, Corey C, Erzurum SC, Shiva S - PLoS ONE (2015)

Asthmatics show airflow obstruction compared to healthy controls.Airflow obstruction as measured by (A) FEV1% predicted and (B) FEV1/FVC in asthmatic individuals compared with healthy control subjects. #p<0.05.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4492492&req=5

pone.0132007.g001: Asthmatics show airflow obstruction compared to healthy controls.Airflow obstruction as measured by (A) FEV1% predicted and (B) FEV1/FVC in asthmatic individuals compared with healthy control subjects. #p<0.05.
Mentions: To investigate platelet metabolism in asthma, a study population including 13 healthy controls and 12 individuals with asthma was utilized (Table 1). The asthmatics had airway reactivity to methacholine, but all asthmatic subjects were non-severe, as defined by criteria of the American Thoracic Society [39]. This was demonstrated by assessment of airflow by measurement of forced expiratory volume in 1 second (FEV1)% predicted and the ratio of FEV1 to forced vital capacity (FVC) (Fig 1). These data demonstrate that although asthmatics had significantly lower airflow compared to control subjects, the airflow limitation was generally mild to moderate [FEV1% predicted: 99 ± 3 (controls) vs 81 ± 5 (asthma), P = 0.01; FEV1/FVC: 0.82 ± 0.02 (controls) vs 0.73 ± 0.03 (asthma), P = 0.03]. Asthmatics were well controlled for at least the prior 6 weeks or longer, and withheld medications prior to day of testing. The number of subjects assessed for each experiment is provided with each result.

Bottom Line: Further, several studies demonstrate altered mitochondrial function in asthmatic airways and suggest that these changes may be systemic.However, it is unknown whether systemic metabolic changes can be detected in circulating cells in asthmatic patients.The implications for this potential metabolic shift will be discussed in the context of increased oxidative stress and hypoxic adaptation of asthmatic patients.

View Article: PubMed Central - PubMed

Affiliation: Lerner Research Institute, Cleveland, Ohio, United States of America.

ABSTRACT
Asthma, a chronic inflammatory airway disease, is typified by high levels of TH2-cytokines and excessive generation of reactive nitrogen and oxygen species, which contribute to bronchial epithelial injury and airway remodeling. While immune function plays a major role in the pathogenesis of the disease, accumulating evidence suggests that altered cellular metabolism is a key determinant in the predisposition and disease progression of asthma. Further, several studies demonstrate altered mitochondrial function in asthmatic airways and suggest that these changes may be systemic. However, it is unknown whether systemic metabolic changes can be detected in circulating cells in asthmatic patients. Platelets are easily accessible blood cells that are known to propagate airway inflammation in asthma. Here we perform a bioenergetic screen of platelets from asthmatic and healthy individuals and demonstrate that asthmatic platelets show a decreased reliance on glycolytic processes and have increased tricarboxylic acid cycle activity. These data demonstrate a systemic alteration in asthma and are consistent with prior reports suggesting that oxidative phosphorylation is more efficient asthmatic individuals. The implications for this potential metabolic shift will be discussed in the context of increased oxidative stress and hypoxic adaptation of asthmatic patients. Further, these data suggest that platelets are potentially a good model for the monitoring of bioenergetic changes in asthma.

No MeSH data available.


Related in: MedlinePlus