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Behavioral assessment of cell transplantation after focal cerebral ischemia in rats.

Lee TH - J Exerc Rehabil (2015)

Bottom Line: Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons.Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect.In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medical Service, Namseoul University, Cheonan, Korea.

ABSTRACT
We induced middle cerebral artery occlusion (MCAO) in rats using silicone-coated vascular embolus. We transplanted mouse embryonic stem (mES) cells after MCAO. Rats were tested behaviorally using motor and sensory function with neurological assessment. Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons. This study demonstrated that the transplanted cells have synaptic connection in the recipient brain. We suggest that stem cell transplantation can have a positive effect on behavioral recovery and reduction of infarct size in focal ischemic rats. Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect. Our study suggests that intracerebrally injected mES cells survived and migrated into the infarct area from inoculation site and neuroglially differentiated in the ischemic brain area of adult rats. Therefore, mES cells may be a useful tool for the treatment in neurological diseases. In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

No MeSH data available.


Related in: MedlinePlus

Behavioral index was tested with PBS treated group and mESCs treated group.
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f3-jer-11-3-140: Behavioral index was tested with PBS treated group and mESCs treated group.

Mentions: Neurological severity scores (NSS) were calculated based on a series of motor, sensory, reflex, and balance tests (Lee and Yoon, 2008). Rats that received PBS show a little behavioral improvement, while transplantation of mESCs transplanted already displayed an early beneficial effect on NSS function and performed significantly better than the control group (Fig. 3). Thus, cell-tranplanted group conferred accelerated recovery in a number of sensorimotor activities. By 28 days after transplantation, group that received mESCs performed significantly better compared to control rats.


Behavioral assessment of cell transplantation after focal cerebral ischemia in rats.

Lee TH - J Exerc Rehabil (2015)

Behavioral index was tested with PBS treated group and mESCs treated group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492423&req=5

f3-jer-11-3-140: Behavioral index was tested with PBS treated group and mESCs treated group.
Mentions: Neurological severity scores (NSS) were calculated based on a series of motor, sensory, reflex, and balance tests (Lee and Yoon, 2008). Rats that received PBS show a little behavioral improvement, while transplantation of mESCs transplanted already displayed an early beneficial effect on NSS function and performed significantly better than the control group (Fig. 3). Thus, cell-tranplanted group conferred accelerated recovery in a number of sensorimotor activities. By 28 days after transplantation, group that received mESCs performed significantly better compared to control rats.

Bottom Line: Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons.Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect.In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medical Service, Namseoul University, Cheonan, Korea.

ABSTRACT
We induced middle cerebral artery occlusion (MCAO) in rats using silicone-coated vascular embolus. We transplanted mouse embryonic stem (mES) cells after MCAO. Rats were tested behaviorally using motor and sensory function with neurological assessment. Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons. This study demonstrated that the transplanted cells have synaptic connection in the recipient brain. We suggest that stem cell transplantation can have a positive effect on behavioral recovery and reduction of infarct size in focal ischemic rats. Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect. Our study suggests that intracerebrally injected mES cells survived and migrated into the infarct area from inoculation site and neuroglially differentiated in the ischemic brain area of adult rats. Therefore, mES cells may be a useful tool for the treatment in neurological diseases. In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

No MeSH data available.


Related in: MedlinePlus