Limits...
Behavioral assessment of cell transplantation after focal cerebral ischemia in rats.

Lee TH - J Exerc Rehabil (2015)

Bottom Line: Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons.Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect.In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medical Service, Namseoul University, Cheonan, Korea.

ABSTRACT
We induced middle cerebral artery occlusion (MCAO) in rats using silicone-coated vascular embolus. We transplanted mouse embryonic stem (mES) cells after MCAO. Rats were tested behaviorally using motor and sensory function with neurological assessment. Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons. This study demonstrated that the transplanted cells have synaptic connection in the recipient brain. We suggest that stem cell transplantation can have a positive effect on behavioral recovery and reduction of infarct size in focal ischemic rats. Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect. Our study suggests that intracerebrally injected mES cells survived and migrated into the infarct area from inoculation site and neuroglially differentiated in the ischemic brain area of adult rats. Therefore, mES cells may be a useful tool for the treatment in neurological diseases. In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

No MeSH data available.


Related in: MedlinePlus

TTC-staining from PBS treated group (A) and mESC treated group (B).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4492423&req=5

f2-jer-11-3-140: TTC-staining from PBS treated group (A) and mESC treated group (B).

Mentions: Normal brain (gray matter) tissue typically stains with TTC, but infarcted lesions show no or reduced staining. TTC staining obtained 4 weeks after MCAO without cell transplantation is shown in Fig. 2A. Fig. 2A showed the reduced TTC staining of the infarction lesion side primarily in the corpus striatum. There were a vivid red staining region and a progressive reduction in infarction size with mESC treatment in Fig. 2B. Intracerebral delivery of mESCs resulted in very substantial reduction in lesion volume as estimated from TTC staining. Cell treatment reduces MCAO-induced brain infarction. Representative TTC stained brain sections are shown where rats were injected with PBS (A; n=18) or mESC (B; n=18) after MCAO. Animals were killed 28 days later after MCAO and the brains were sliced into 2 mm sections and stained with 2,3,5-triphenyltetrazolium chloride (TTC). Different volumes in infarcted region of brains from PBS and mESC treated animals are shown in the Fig. 2A and 2B respectively.


Behavioral assessment of cell transplantation after focal cerebral ischemia in rats.

Lee TH - J Exerc Rehabil (2015)

TTC-staining from PBS treated group (A) and mESC treated group (B).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492423&req=5

f2-jer-11-3-140: TTC-staining from PBS treated group (A) and mESC treated group (B).
Mentions: Normal brain (gray matter) tissue typically stains with TTC, but infarcted lesions show no or reduced staining. TTC staining obtained 4 weeks after MCAO without cell transplantation is shown in Fig. 2A. Fig. 2A showed the reduced TTC staining of the infarction lesion side primarily in the corpus striatum. There were a vivid red staining region and a progressive reduction in infarction size with mESC treatment in Fig. 2B. Intracerebral delivery of mESCs resulted in very substantial reduction in lesion volume as estimated from TTC staining. Cell treatment reduces MCAO-induced brain infarction. Representative TTC stained brain sections are shown where rats were injected with PBS (A; n=18) or mESC (B; n=18) after MCAO. Animals were killed 28 days later after MCAO and the brains were sliced into 2 mm sections and stained with 2,3,5-triphenyltetrazolium chloride (TTC). Different volumes in infarcted region of brains from PBS and mESC treated animals are shown in the Fig. 2A and 2B respectively.

Bottom Line: Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons.Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect.In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medical Service, Namseoul University, Cheonan, Korea.

ABSTRACT
We induced middle cerebral artery occlusion (MCAO) in rats using silicone-coated vascular embolus. We transplanted mouse embryonic stem (mES) cells after MCAO. Rats were tested behaviorally using motor and sensory function with neurological assessment. Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons. This study demonstrated that the transplanted cells have synaptic connection in the recipient brain. We suggest that stem cell transplantation can have a positive effect on behavioral recovery and reduction of infarct size in focal ischemic rats. Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect. Our study suggests that intracerebrally injected mES cells survived and migrated into the infarct area from inoculation site and neuroglially differentiated in the ischemic brain area of adult rats. Therefore, mES cells may be a useful tool for the treatment in neurological diseases. In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

No MeSH data available.


Related in: MedlinePlus