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Behavioral assessment of cell transplantation after focal cerebral ischemia in rats.

Lee TH - J Exerc Rehabil (2015)

Bottom Line: Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons.Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect.In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medical Service, Namseoul University, Cheonan, Korea.

ABSTRACT
We induced middle cerebral artery occlusion (MCAO) in rats using silicone-coated vascular embolus. We transplanted mouse embryonic stem (mES) cells after MCAO. Rats were tested behaviorally using motor and sensory function with neurological assessment. Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons. This study demonstrated that the transplanted cells have synaptic connection in the recipient brain. We suggest that stem cell transplantation can have a positive effect on behavioral recovery and reduction of infarct size in focal ischemic rats. Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect. Our study suggests that intracerebrally injected mES cells survived and migrated into the infarct area from inoculation site and neuroglially differentiated in the ischemic brain area of adult rats. Therefore, mES cells may be a useful tool for the treatment in neurological diseases. In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

No MeSH data available.


Related in: MedlinePlus

Morphological features of mES cells showed green fluorescence in vitro (Left and middle: X-100 magnification, Right: X-200 magnification).
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f1-jer-11-3-140: Morphological features of mES cells showed green fluorescence in vitro (Left and middle: X-100 magnification, Right: X-200 magnification).

Mentions: White color of infarction region was shown mainly in corpus callosum and striatum. Fig. 1 shows severe inflammation of infarction site with cytotoxic edema in day 5 after MCAO. fMR imaging technique by diffusion coefficient can be recognized specific infarction region in vitro.


Behavioral assessment of cell transplantation after focal cerebral ischemia in rats.

Lee TH - J Exerc Rehabil (2015)

Morphological features of mES cells showed green fluorescence in vitro (Left and middle: X-100 magnification, Right: X-200 magnification).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492423&req=5

f1-jer-11-3-140: Morphological features of mES cells showed green fluorescence in vitro (Left and middle: X-100 magnification, Right: X-200 magnification).
Mentions: White color of infarction region was shown mainly in corpus callosum and striatum. Fig. 1 shows severe inflammation of infarction site with cytotoxic edema in day 5 after MCAO. fMR imaging technique by diffusion coefficient can be recognized specific infarction region in vitro.

Bottom Line: Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons.Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect.In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

View Article: PubMed Central - PubMed

Affiliation: Department of Emergency Medical Service, Namseoul University, Cheonan, Korea.

ABSTRACT
We induced middle cerebral artery occlusion (MCAO) in rats using silicone-coated vascular embolus. We transplanted mouse embryonic stem (mES) cells after MCAO. Rats were tested behaviorally using motor and sensory function with neurological assessment. Functional effectiveness of the transplanted mES cells gradually improved the function of sensory and motor neurons. This study demonstrated that the transplanted cells have synaptic connection in the recipient brain. We suggest that stem cell transplantation can have a positive effect on behavioral recovery and reduction of infarct size in focal ischemic rats. Cell transplantation may induce certain functional recovery of the brain tissue by endogenous cell mediated effect. Our study suggests that intracerebrally injected mES cells survived and migrated into the infarct area from inoculation site and neuroglially differentiated in the ischemic brain area of adult rats. Therefore, mES cells may be a useful tool for the treatment in neurological diseases. In conclusion, cell transplantation therapy represents a novel approach that may enhance the efficacy and effectiveness of stem cell transplantation after ischemic stroke.

No MeSH data available.


Related in: MedlinePlus