A Transparent Window into Biology: A Primer on Caenorhabditis elegans.
Bottom Line: We survey the basic anatomical features, common technical approaches, and important discoveries in C. elegans research.Key to studying C. elegans has been the ability to address biological problems genetically, using both forward and reverse genetics, both at the level of the entire organism and at the level of the single, identified cell.These possibilities make C. elegans useful not only in research laboratories, but also in the classroom where it can be used to excite students who actually can see what is happening inside live cells and tissues.
Affiliation: Biology Department, The Catholic University of America, Washington, DC 20064 firstname.lastname@example.org email@example.com firstname.lastname@example.org.Show MeSH
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Mentions: Mutations in several genes needed for cuticle formation produce visible phenotypes (Figure 6). Mutations in collagen genes can result in animals that move in a corkscrew fashion [the Roller (Rol) phenotype] or that have normal width but reduced length [the Dumpy (Dpy) phenotype]. Other mutations affect the struts formed between layers of the adult cuticle, resulting in fluid-filled blisters [the Blister (Bli) phenotype]. Still other mutations make the animals longer than normal [the Long (Lon) phenotype]. At the end of each larval stage (Figure 2), C. elegans sheds its cuticle and secretes a new one to accommodate the growing organism. Genes involved in cuticle formation are regulated so that the cuticle is reestablished after each molt.
Affiliation: Biology Department, The Catholic University of America, Washington, DC 20064 email@example.com firstname.lastname@example.org email@example.com.