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The Sasa quelpaertensis Leaf Extract Inhibits the Dextran Sulfate Sodium-induced Mouse Colitis Through Modulation of Antioxidant Enzyme Expression.

Yeom Y, Kim Y - J Cancer Prev (2015)

Bottom Line: Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group.In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group.The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, Korea.

ABSTRACT

Background: Oxidative stress plays an important role in the pathogenesis of inflammatory bowel disease. The objective of this study is to investigate the protective effect of Sasa quelpaertensis leaf extract (SQE) against oxidative stress in mice with dextran sulfate sodium (DSS)-induced colitis.

Methods: Mice were treated with SQE (100 mg/kg or 300 mg/kg body weight) by gavage in advance two weeks before inflammation was induced. Then, the mice were administered with 2.5% DSS in drinking water for 7 days and normal drinking water for 7 days between two DSS treatment. Disease activity index values, gut motility, and severity of the resulting oxidative DNA damage were analyzed. The antioxidant effect of SQE was evaluated by measuring malondialdehyde (MDA) and superoxide dismutase (SOD) activity in plasma samples. Catalase activity and expressions levels of glutathione peroxidase 1 (Gpx1), SOD1, and SOD2 were also detected in colon tissues.

Results: Administration of SQE significantly reduced the severity of DSS-induced colitis compared to the control (Ctrl) group. Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group. In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group. The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group. In contrast, administration of SQE significantly down-regulated SOD2 and Gpx1 expressions and up-regulated SOD1 expression.

Conclusions: These results indicate that SQE efficiently suppresses oxidative stress in DSS-induced colitis in mice, and its action is associated with the regulation of antioxidant enzymes.

No MeSH data available.


Related in: MedlinePlus

SQE administration regulates expression of SOD and Gpx1 in the colon of mice treated with DSS. Expressions of SOD1 (A), SOD2 (B), and Gpx1 (C) were analyzed in colon tissues by using western blot. Level of α-tubulin was detected as a loading control. Band intensities were quantified by densitometry and data shown are the mean ± SEM and were used were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; SOD, superoxide dismutase; Gpx1, glutathione peroxidase 1; Ctrl, control; DSS, dextran sulfate sodium; SSZ, sulfasalazine.
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f5-jcp-20-136: SQE administration regulates expression of SOD and Gpx1 in the colon of mice treated with DSS. Expressions of SOD1 (A), SOD2 (B), and Gpx1 (C) were analyzed in colon tissues by using western blot. Level of α-tubulin was detected as a loading control. Band intensities were quantified by densitometry and data shown are the mean ± SEM and were used were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; SOD, superoxide dismutase; Gpx1, glutathione peroxidase 1; Ctrl, control; DSS, dextran sulfate sodium; SSZ, sulfasalazine.

Mentions: To investigate the role of SQE administration in regulating oxidative stress in a mouse model of DSS-induced colitis, the expression levels of several antioxidant enzymes, SOD1 (Cu-Zn SOD), SOD2 (Mn SOD), and Gpx1, were analyzed. For the DSS group, the levels of SOD1 expression decreased by 27.2% compared with the Ctrl group (P < 0.01). However, the SOD1 expression levels were significantly higher in both of the SQE groups compared to the DSS Ctrl group (Fig. 5A). SSZ treatment did not affect SOD expression. In contrast, the enzyme activity levels of SOD2 and Gpx1 in colon tissues from the DSS Ctrl group were higher than those of the Ctrl group. In the SQE 300 tissues, the enzyme expressions of SOD2 and Gpx1 were significantly lower by 31.9% (P < 0.001) and 27.3% (P < 0.01), respectively, compared with the DSS Ctrl group (Fig. 5B and 5C). These results suggest that SQE administration regulates the expression levels of certain antioxidant enzymes that are involved in a DSS-induced colitis in mice.


The Sasa quelpaertensis Leaf Extract Inhibits the Dextran Sulfate Sodium-induced Mouse Colitis Through Modulation of Antioxidant Enzyme Expression.

Yeom Y, Kim Y - J Cancer Prev (2015)

SQE administration regulates expression of SOD and Gpx1 in the colon of mice treated with DSS. Expressions of SOD1 (A), SOD2 (B), and Gpx1 (C) were analyzed in colon tissues by using western blot. Level of α-tubulin was detected as a loading control. Band intensities were quantified by densitometry and data shown are the mean ± SEM and were used were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; SOD, superoxide dismutase; Gpx1, glutathione peroxidase 1; Ctrl, control; DSS, dextran sulfate sodium; SSZ, sulfasalazine.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4492358&req=5

f5-jcp-20-136: SQE administration regulates expression of SOD and Gpx1 in the colon of mice treated with DSS. Expressions of SOD1 (A), SOD2 (B), and Gpx1 (C) were analyzed in colon tissues by using western blot. Level of α-tubulin was detected as a loading control. Band intensities were quantified by densitometry and data shown are the mean ± SEM and were used were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; SOD, superoxide dismutase; Gpx1, glutathione peroxidase 1; Ctrl, control; DSS, dextran sulfate sodium; SSZ, sulfasalazine.
Mentions: To investigate the role of SQE administration in regulating oxidative stress in a mouse model of DSS-induced colitis, the expression levels of several antioxidant enzymes, SOD1 (Cu-Zn SOD), SOD2 (Mn SOD), and Gpx1, were analyzed. For the DSS group, the levels of SOD1 expression decreased by 27.2% compared with the Ctrl group (P < 0.01). However, the SOD1 expression levels were significantly higher in both of the SQE groups compared to the DSS Ctrl group (Fig. 5A). SSZ treatment did not affect SOD expression. In contrast, the enzyme activity levels of SOD2 and Gpx1 in colon tissues from the DSS Ctrl group were higher than those of the Ctrl group. In the SQE 300 tissues, the enzyme expressions of SOD2 and Gpx1 were significantly lower by 31.9% (P < 0.001) and 27.3% (P < 0.01), respectively, compared with the DSS Ctrl group (Fig. 5B and 5C). These results suggest that SQE administration regulates the expression levels of certain antioxidant enzymes that are involved in a DSS-induced colitis in mice.

Bottom Line: Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group.In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group.The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, Korea.

ABSTRACT

Background: Oxidative stress plays an important role in the pathogenesis of inflammatory bowel disease. The objective of this study is to investigate the protective effect of Sasa quelpaertensis leaf extract (SQE) against oxidative stress in mice with dextran sulfate sodium (DSS)-induced colitis.

Methods: Mice were treated with SQE (100 mg/kg or 300 mg/kg body weight) by gavage in advance two weeks before inflammation was induced. Then, the mice were administered with 2.5% DSS in drinking water for 7 days and normal drinking water for 7 days between two DSS treatment. Disease activity index values, gut motility, and severity of the resulting oxidative DNA damage were analyzed. The antioxidant effect of SQE was evaluated by measuring malondialdehyde (MDA) and superoxide dismutase (SOD) activity in plasma samples. Catalase activity and expressions levels of glutathione peroxidase 1 (Gpx1), SOD1, and SOD2 were also detected in colon tissues.

Results: Administration of SQE significantly reduced the severity of DSS-induced colitis compared to the control (Ctrl) group. Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group. In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group. The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group. In contrast, administration of SQE significantly down-regulated SOD2 and Gpx1 expressions and up-regulated SOD1 expression.

Conclusions: These results indicate that SQE efficiently suppresses oxidative stress in DSS-induced colitis in mice, and its action is associated with the regulation of antioxidant enzymes.

No MeSH data available.


Related in: MedlinePlus