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The Sasa quelpaertensis Leaf Extract Inhibits the Dextran Sulfate Sodium-induced Mouse Colitis Through Modulation of Antioxidant Enzyme Expression.

Yeom Y, Kim Y - J Cancer Prev (2015)

Bottom Line: Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group.In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group.The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, Korea.

ABSTRACT

Background: Oxidative stress plays an important role in the pathogenesis of inflammatory bowel disease. The objective of this study is to investigate the protective effect of Sasa quelpaertensis leaf extract (SQE) against oxidative stress in mice with dextran sulfate sodium (DSS)-induced colitis.

Methods: Mice were treated with SQE (100 mg/kg or 300 mg/kg body weight) by gavage in advance two weeks before inflammation was induced. Then, the mice were administered with 2.5% DSS in drinking water for 7 days and normal drinking water for 7 days between two DSS treatment. Disease activity index values, gut motility, and severity of the resulting oxidative DNA damage were analyzed. The antioxidant effect of SQE was evaluated by measuring malondialdehyde (MDA) and superoxide dismutase (SOD) activity in plasma samples. Catalase activity and expressions levels of glutathione peroxidase 1 (Gpx1), SOD1, and SOD2 were also detected in colon tissues.

Results: Administration of SQE significantly reduced the severity of DSS-induced colitis compared to the control (Ctrl) group. Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group. In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group. The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group. In contrast, administration of SQE significantly down-regulated SOD2 and Gpx1 expressions and up-regulated SOD1 expression.

Conclusions: These results indicate that SQE efficiently suppresses oxidative stress in DSS-induced colitis in mice, and its action is associated with the regulation of antioxidant enzymes.

No MeSH data available.


Related in: MedlinePlus

Administration of SQE regulates antioxidant enzyme activity in DSS-induced colitis mice. Levels of MDA (A) and SOD (B) were examined by commercial kit in plasma for all groups. (C) Catalase activity was examined in colon tissues by using a commercial kit. Data are shown to as the mean ± SEM and were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; DSS, dextran sulfate sodium; MDA, malondialdehyde; SOD, superoxide dismutase; conc., concentration; Ctrl, control; SSZ, sulfasalazine.
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f4-jcp-20-136: Administration of SQE regulates antioxidant enzyme activity in DSS-induced colitis mice. Levels of MDA (A) and SOD (B) were examined by commercial kit in plasma for all groups. (C) Catalase activity was examined in colon tissues by using a commercial kit. Data are shown to as the mean ± SEM and were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; DSS, dextran sulfate sodium; MDA, malondialdehyde; SOD, superoxide dismutase; conc., concentration; Ctrl, control; SSZ, sulfasalazine.

Mentions: To evaluate the antioxidative capacity of SQE administration, MDA and SOD activity levels in plasma samples, and catalase activity in colon tissues were analyzed. MDA activity in the SQE 300 group was significantly lower by 28.1% compared with that of the DSS Ctrl group (Fig. 4A; P < 0.05), and there it was no statistically significant difference between the MDA activity of the SSZ group and the DSS Ctrl group. Expression levels of SOD were lower by 20.7% in the DSS Ctrl group compared with the Ctrl group (Fig. 4B), whereas the SOD levels were increased by 28.1% in the SQE 300 group compared to the DSS Ctrl group (P < 0.01). Both of these differences were significant. In combination, these results indicate that SQE protects the levels of SOD activity in the colon tissues of mice with DSS-induced colitis. Regarding the levels of catalase, the lowest levels were detected in the colon tissues of the DSS Ctrl group (Fig. 4C). In contrast, the catalase activity level of the SQE 300 group was significantly increased by 44.7% compared with the DSS Ctrl group (P < 0.05). Overall, these results indicate that SQE regulates antioxidant enzyme activity against oxidative stress in DSS-induced colitis in mice.


The Sasa quelpaertensis Leaf Extract Inhibits the Dextran Sulfate Sodium-induced Mouse Colitis Through Modulation of Antioxidant Enzyme Expression.

Yeom Y, Kim Y - J Cancer Prev (2015)

Administration of SQE regulates antioxidant enzyme activity in DSS-induced colitis mice. Levels of MDA (A) and SOD (B) were examined by commercial kit in plasma for all groups. (C) Catalase activity was examined in colon tissues by using a commercial kit. Data are shown to as the mean ± SEM and were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; DSS, dextran sulfate sodium; MDA, malondialdehyde; SOD, superoxide dismutase; conc., concentration; Ctrl, control; SSZ, sulfasalazine.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4492358&req=5

f4-jcp-20-136: Administration of SQE regulates antioxidant enzyme activity in DSS-induced colitis mice. Levels of MDA (A) and SOD (B) were examined by commercial kit in plasma for all groups. (C) Catalase activity was examined in colon tissues by using a commercial kit. Data are shown to as the mean ± SEM and were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; DSS, dextran sulfate sodium; MDA, malondialdehyde; SOD, superoxide dismutase; conc., concentration; Ctrl, control; SSZ, sulfasalazine.
Mentions: To evaluate the antioxidative capacity of SQE administration, MDA and SOD activity levels in plasma samples, and catalase activity in colon tissues were analyzed. MDA activity in the SQE 300 group was significantly lower by 28.1% compared with that of the DSS Ctrl group (Fig. 4A; P < 0.05), and there it was no statistically significant difference between the MDA activity of the SSZ group and the DSS Ctrl group. Expression levels of SOD were lower by 20.7% in the DSS Ctrl group compared with the Ctrl group (Fig. 4B), whereas the SOD levels were increased by 28.1% in the SQE 300 group compared to the DSS Ctrl group (P < 0.01). Both of these differences were significant. In combination, these results indicate that SQE protects the levels of SOD activity in the colon tissues of mice with DSS-induced colitis. Regarding the levels of catalase, the lowest levels were detected in the colon tissues of the DSS Ctrl group (Fig. 4C). In contrast, the catalase activity level of the SQE 300 group was significantly increased by 44.7% compared with the DSS Ctrl group (P < 0.05). Overall, these results indicate that SQE regulates antioxidant enzyme activity against oxidative stress in DSS-induced colitis in mice.

Bottom Line: Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group.In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group.The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, Korea.

ABSTRACT

Background: Oxidative stress plays an important role in the pathogenesis of inflammatory bowel disease. The objective of this study is to investigate the protective effect of Sasa quelpaertensis leaf extract (SQE) against oxidative stress in mice with dextran sulfate sodium (DSS)-induced colitis.

Methods: Mice were treated with SQE (100 mg/kg or 300 mg/kg body weight) by gavage in advance two weeks before inflammation was induced. Then, the mice were administered with 2.5% DSS in drinking water for 7 days and normal drinking water for 7 days between two DSS treatment. Disease activity index values, gut motility, and severity of the resulting oxidative DNA damage were analyzed. The antioxidant effect of SQE was evaluated by measuring malondialdehyde (MDA) and superoxide dismutase (SOD) activity in plasma samples. Catalase activity and expressions levels of glutathione peroxidase 1 (Gpx1), SOD1, and SOD2 were also detected in colon tissues.

Results: Administration of SQE significantly reduced the severity of DSS-induced colitis compared to the control (Ctrl) group. Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group. In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group. The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group. In contrast, administration of SQE significantly down-regulated SOD2 and Gpx1 expressions and up-regulated SOD1 expression.

Conclusions: These results indicate that SQE efficiently suppresses oxidative stress in DSS-induced colitis in mice, and its action is associated with the regulation of antioxidant enzymes.

No MeSH data available.


Related in: MedlinePlus