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The Sasa quelpaertensis Leaf Extract Inhibits the Dextran Sulfate Sodium-induced Mouse Colitis Through Modulation of Antioxidant Enzyme Expression.

Yeom Y, Kim Y - J Cancer Prev (2015)

Bottom Line: Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group.In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group.The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, Korea.

ABSTRACT

Background: Oxidative stress plays an important role in the pathogenesis of inflammatory bowel disease. The objective of this study is to investigate the protective effect of Sasa quelpaertensis leaf extract (SQE) against oxidative stress in mice with dextran sulfate sodium (DSS)-induced colitis.

Methods: Mice were treated with SQE (100 mg/kg or 300 mg/kg body weight) by gavage in advance two weeks before inflammation was induced. Then, the mice were administered with 2.5% DSS in drinking water for 7 days and normal drinking water for 7 days between two DSS treatment. Disease activity index values, gut motility, and severity of the resulting oxidative DNA damage were analyzed. The antioxidant effect of SQE was evaluated by measuring malondialdehyde (MDA) and superoxide dismutase (SOD) activity in plasma samples. Catalase activity and expressions levels of glutathione peroxidase 1 (Gpx1), SOD1, and SOD2 were also detected in colon tissues.

Results: Administration of SQE significantly reduced the severity of DSS-induced colitis compared to the control (Ctrl) group. Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group. In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group. The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group. In contrast, administration of SQE significantly down-regulated SOD2 and Gpx1 expressions and up-regulated SOD1 expression.

Conclusions: These results indicate that SQE efficiently suppresses oxidative stress in DSS-induced colitis in mice, and its action is associated with the regulation of antioxidant enzymes.

No MeSH data available.


Related in: MedlinePlus

Administration of SQE alleviates oxidative DNA damage caused by DSS in the mouse colon. (A) Production of 8-oxo-dG was detected using immunohistochemistry in sections of colorectal tissue obtained DSS-induced colitis mice for: (a) Ctrl, (b) DSS Ctrl, (c) SSZ, (d) SQE 100, and (e) SQE 300 (a–e: × 400). (B) The percent of positive cells from 3 fields were counted and averaged. Scale bar: 50 μm. Data are shown to as the mean ± SEM and were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; Ctrl, control; DSS, dextran sulfate sodium; SSZ, sulfasalazine.
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f3-jcp-20-136: Administration of SQE alleviates oxidative DNA damage caused by DSS in the mouse colon. (A) Production of 8-oxo-dG was detected using immunohistochemistry in sections of colorectal tissue obtained DSS-induced colitis mice for: (a) Ctrl, (b) DSS Ctrl, (c) SSZ, (d) SQE 100, and (e) SQE 300 (a–e: × 400). (B) The percent of positive cells from 3 fields were counted and averaged. Scale bar: 50 μm. Data are shown to as the mean ± SEM and were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; Ctrl, control; DSS, dextran sulfate sodium; SSZ, sulfasalazine.

Mentions: The reaction of an OH radical with a DNA guanosine base generates 8-oxo-dG.33 Thus, detection of 8-oxo-dG has been used as a marker of DNA damage. The percentage of positively stained epithelial cells with 8-oxo-dG was significantly higher in the colon tissues of the DSS Ctrl group (49.8%) compared with the tissues from the Ctrl group (Fig. 3). SSZ treatment did not affect the number of 8-oxo-dG-positive cells compared to the DSS Ctrl group, while administration of SQE significantly reduced the percentage of 8-oxo-dG-positive cells compared to the DSS Ctrl group (P < 0.05). In particular, the percentage of 8-oxo-dG-positive cells in the SQE 300 tissues was significantly decreased by 36.1% compared to that of the DSS Ctrl group tissues (P < 0.05), and this percentage was similar to that of the Ctrl group. Taken together, these results suggest that SQE treatment protects against DNA damage from DSS-induced colitis in mice.


The Sasa quelpaertensis Leaf Extract Inhibits the Dextran Sulfate Sodium-induced Mouse Colitis Through Modulation of Antioxidant Enzyme Expression.

Yeom Y, Kim Y - J Cancer Prev (2015)

Administration of SQE alleviates oxidative DNA damage caused by DSS in the mouse colon. (A) Production of 8-oxo-dG was detected using immunohistochemistry in sections of colorectal tissue obtained DSS-induced colitis mice for: (a) Ctrl, (b) DSS Ctrl, (c) SSZ, (d) SQE 100, and (e) SQE 300 (a–e: × 400). (B) The percent of positive cells from 3 fields were counted and averaged. Scale bar: 50 μm. Data are shown to as the mean ± SEM and were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; Ctrl, control; DSS, dextran sulfate sodium; SSZ, sulfasalazine.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492358&req=5

f3-jcp-20-136: Administration of SQE alleviates oxidative DNA damage caused by DSS in the mouse colon. (A) Production of 8-oxo-dG was detected using immunohistochemistry in sections of colorectal tissue obtained DSS-induced colitis mice for: (a) Ctrl, (b) DSS Ctrl, (c) SSZ, (d) SQE 100, and (e) SQE 300 (a–e: × 400). (B) The percent of positive cells from 3 fields were counted and averaged. Scale bar: 50 μm. Data are shown to as the mean ± SEM and were analyzed using one-way ANOVA and Tukey’s post-hoc test (P < 0.05); n = 10 mice per group. Different letters are used to indicate significant differences. SQE, Sasa quelpaertensis extract; Ctrl, control; DSS, dextran sulfate sodium; SSZ, sulfasalazine.
Mentions: The reaction of an OH radical with a DNA guanosine base generates 8-oxo-dG.33 Thus, detection of 8-oxo-dG has been used as a marker of DNA damage. The percentage of positively stained epithelial cells with 8-oxo-dG was significantly higher in the colon tissues of the DSS Ctrl group (49.8%) compared with the tissues from the Ctrl group (Fig. 3). SSZ treatment did not affect the number of 8-oxo-dG-positive cells compared to the DSS Ctrl group, while administration of SQE significantly reduced the percentage of 8-oxo-dG-positive cells compared to the DSS Ctrl group (P < 0.05). In particular, the percentage of 8-oxo-dG-positive cells in the SQE 300 tissues was significantly decreased by 36.1% compared to that of the DSS Ctrl group tissues (P < 0.05), and this percentage was similar to that of the Ctrl group. Taken together, these results suggest that SQE treatment protects against DNA damage from DSS-induced colitis in mice.

Bottom Line: Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group.In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group.The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutritional Science and Food Management, College of Health Sciences, Ewha Womans University, Seoul, Korea.

ABSTRACT

Background: Oxidative stress plays an important role in the pathogenesis of inflammatory bowel disease. The objective of this study is to investigate the protective effect of Sasa quelpaertensis leaf extract (SQE) against oxidative stress in mice with dextran sulfate sodium (DSS)-induced colitis.

Methods: Mice were treated with SQE (100 mg/kg or 300 mg/kg body weight) by gavage in advance two weeks before inflammation was induced. Then, the mice were administered with 2.5% DSS in drinking water for 7 days and normal drinking water for 7 days between two DSS treatment. Disease activity index values, gut motility, and severity of the resulting oxidative DNA damage were analyzed. The antioxidant effect of SQE was evaluated by measuring malondialdehyde (MDA) and superoxide dismutase (SOD) activity in plasma samples. Catalase activity and expressions levels of glutathione peroxidase 1 (Gpx1), SOD1, and SOD2 were also detected in colon tissues.

Results: Administration of SQE significantly reduced the severity of DSS-induced colitis compared to the control (Ctrl) group. Levels of 8-oxo-dG, an oxidative DNA damage marker, were significantly lower in the SQE group compared to the untreated DSS Ctrl group. In the SQE (300 mg/kg) group, MDA levels were significantly lower, while SOD and catalase activity levels in the plasma samples were significantly higher compared with the DSS Ctrl group. The expression levels of the antioxidant enzymes, SOD2 and Gpx1, were significantly higher, while the levels of SOD 1 expression were lower, in the colon tissues of the DSS Ctrl group compared with those of the Ctrl group. In contrast, administration of SQE significantly down-regulated SOD2 and Gpx1 expressions and up-regulated SOD1 expression.

Conclusions: These results indicate that SQE efficiently suppresses oxidative stress in DSS-induced colitis in mice, and its action is associated with the regulation of antioxidant enzymes.

No MeSH data available.


Related in: MedlinePlus