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Isolation, purification and characterization of novel antimicrobial compound 7-methoxy-2,2-dimethyl-4-octa-4',6'-dienyl-2H-napthalene-1-one from Penicillium sp. and its cytotoxicity studies.

Kaur H, Onsare JG, Sharma V, Arora DS - AMB Express (2015)

Bottom Line: MIC of the active compound ranged from (0.5-15 µg/mL which was found to be comparable with the standard antibiotics.Further, the compound when tested for its biosafety was found neither to be cytotoxic nor mutagenic.The study demonstrated that an apparently novel compound isolated from Penicillium sp. seems to be a stable and potent antimicrobial.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Microbial Technology Laboratory, Guru Nanak Dev University, Amritsar, 143005, India, harpreet40@gmail.com.

ABSTRACT
Fungus isolated from soil has been evaluated for its antimicrobial activity which showed broad spectrum antimicrobial activity against all the pathogenic microorganisms used. Optimization was done by response surface methodology (RSM) to further optimize the medium which could further enhance the antimicrobial activity by 1.1-1.9 folds. Column chromatography was used to isolate the active compound which was characterized to be by various spectroscopic techniques such NMR, IR and LCMS and it was found to be apparently novel compound 7-methoxy-2,2-dimethyl-4-octa-4',6'-dienyl-2 H -napthalene-1-one. MIC of the active compound ranged from (0.5-15 µg/mL which was found to be comparable with the standard antibiotics. Viable cell count studies of the active compound showed it to be bactericidal in nature. Further, the compound when tested for its biosafety was found neither to be cytotoxic nor mutagenic. Cytotoxicity studies of the compound on cancer cell lines showed a valuable cytotoxic potential against all tested human cancer cell lines. Further, the compound induces apoptosis in lung cancer (A549) cells reveled by increase the distribution of nuclear DNA in Sub G1 phase as observed in flow cytometry. The study demonstrated that an apparently novel compound isolated from Penicillium sp. seems to be a stable and potent antimicrobial.

No MeSH data available.


Related in: MedlinePlus

Flow cytometric analysis of nuclear DNA treated compound and thestandard.
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Fig6: Flow cytometric analysis of nuclear DNA treated compound and thestandard.

Mentions: Morphological change is a primary indication of cell apoptosis. Oneof the specific characteristics of cell apoptosis is DNA fragmentation, whichleads to the appearance of hypodiploid cells. The cell cycle is a key factor inunregulated cell proliferation leading to cancer and differential sensitivity toapoptosis is linked to the distinct phases of cell cycle (Pucci et al. 2000).The hypo diploid sub-G1 DNA fraction (<2n DNA) was found to increase from 37 to58% for compound in a concentration dependent manner (Figures 5, 6). Incontrast to camptothecin (positive control) showed 48% hypo diploid DNA content ata concentration of 2 µM.Figure 5


Isolation, purification and characterization of novel antimicrobial compound 7-methoxy-2,2-dimethyl-4-octa-4',6'-dienyl-2H-napthalene-1-one from Penicillium sp. and its cytotoxicity studies.

Kaur H, Onsare JG, Sharma V, Arora DS - AMB Express (2015)

Flow cytometric analysis of nuclear DNA treated compound and thestandard.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492345&req=5

Fig6: Flow cytometric analysis of nuclear DNA treated compound and thestandard.
Mentions: Morphological change is a primary indication of cell apoptosis. Oneof the specific characteristics of cell apoptosis is DNA fragmentation, whichleads to the appearance of hypodiploid cells. The cell cycle is a key factor inunregulated cell proliferation leading to cancer and differential sensitivity toapoptosis is linked to the distinct phases of cell cycle (Pucci et al. 2000).The hypo diploid sub-G1 DNA fraction (<2n DNA) was found to increase from 37 to58% for compound in a concentration dependent manner (Figures 5, 6). Incontrast to camptothecin (positive control) showed 48% hypo diploid DNA content ata concentration of 2 µM.Figure 5

Bottom Line: MIC of the active compound ranged from (0.5-15 µg/mL which was found to be comparable with the standard antibiotics.Further, the compound when tested for its biosafety was found neither to be cytotoxic nor mutagenic.The study demonstrated that an apparently novel compound isolated from Penicillium sp. seems to be a stable and potent antimicrobial.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Microbial Technology Laboratory, Guru Nanak Dev University, Amritsar, 143005, India, harpreet40@gmail.com.

ABSTRACT
Fungus isolated from soil has been evaluated for its antimicrobial activity which showed broad spectrum antimicrobial activity against all the pathogenic microorganisms used. Optimization was done by response surface methodology (RSM) to further optimize the medium which could further enhance the antimicrobial activity by 1.1-1.9 folds. Column chromatography was used to isolate the active compound which was characterized to be by various spectroscopic techniques such NMR, IR and LCMS and it was found to be apparently novel compound 7-methoxy-2,2-dimethyl-4-octa-4',6'-dienyl-2 H -napthalene-1-one. MIC of the active compound ranged from (0.5-15 µg/mL which was found to be comparable with the standard antibiotics. Viable cell count studies of the active compound showed it to be bactericidal in nature. Further, the compound when tested for its biosafety was found neither to be cytotoxic nor mutagenic. Cytotoxicity studies of the compound on cancer cell lines showed a valuable cytotoxic potential against all tested human cancer cell lines. Further, the compound induces apoptosis in lung cancer (A549) cells reveled by increase the distribution of nuclear DNA in Sub G1 phase as observed in flow cytometry. The study demonstrated that an apparently novel compound isolated from Penicillium sp. seems to be a stable and potent antimicrobial.

No MeSH data available.


Related in: MedlinePlus