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The Mycetophilaruficollis Meigen (Diptera, Mycetophilidae) group in Europe: elucidating species delimitation with COI and ITS2 sequence data.

Jürgenstein S, Kurina O, Põldmaa K - Zookeys (2015)

Bottom Line: European species of the Mycetophilaruficollis group are compared on the basis of morphology and sequences of mitochondrial cytochrome oxidase subunit one (COI) and the ITS2 region of nuclear ribosomal DNA.New country records, viz.Estonia for Mycetophilaevanida, Georgia for Mycetophilaichneumonea, Mycetophilaidonea and Mycetophilaruficollis, and Norway for Mycetophilastrobli, widen the known distribution ranges of these species.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi st 5D, 51014 Tartu, ESTONIA.

ABSTRACT
European species of the Mycetophilaruficollis group are compared on the basis of morphology and sequences of mitochondrial cytochrome oxidase subunit one (COI) and the ITS2 region of nuclear ribosomal DNA. The study represents the first evaluation of morphology-based species delimitation of closely related fungus gnat species by applying molecular information. Detailed descriptions and illustrations of the male terminalia are presented along with a key for the identification of all nine European species of the group. Phylogenetic analyses of molecular data generally supported the morphological species discrimination. The barcoding region of COI superseded ITS2 rDNA in resolving species. In the COI barcoding region interspecific differences ranged from 2.9 to 10.6% and the intraspecific distance from 0.08 to 0.8%. Only COI data distinguished between the similar and closely related Mycetophilaichneumonea and Mycetophilauninotata of which the latter was observed to include cryptic species. The host range of some species is suggested to be narrower than previously considered and to depend on the forest type. Presented evidence indicates the importance of analysing sequence data of morphologically very similar mycetophages reared from identified host fungi for elucidating species delimitation as well as their geographic and host ranges. New country records, viz. Estonia for Mycetophilaevanida, Georgia for Mycetophilaichneumonea, Mycetophilaidonea and Mycetophilaruficollis, and Norway for Mycetophilastrobli, widen the known distribution ranges of these species.

No MeSH data available.


Ventral branch of gonostylus, ventral view (15, 17, 19) and internal view (16, 18, 20). 15, 16Mycetophilabritannica17, 18Mycetophilaidonea19, 20Mycetophilaichneumonea. Scale bar = 0.05 mm. Abbreviations: pp = posterior process; sp = posterior spines on the ventral branch of gonostylus.
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Figure 5: Ventral branch of gonostylus, ventral view (15, 17, 19) and internal view (16, 18, 20). 15, 16Mycetophilabritannica17, 18Mycetophilaidonea19, 20Mycetophilaichneumonea. Scale bar = 0.05 mm. Abbreviations: pp = posterior process; sp = posterior spines on the ventral branch of gonostylus.

Mentions: Terminology used for describing male terminalia with synonyms from earlier studies and references to corresponding figures.


The Mycetophilaruficollis Meigen (Diptera, Mycetophilidae) group in Europe: elucidating species delimitation with COI and ITS2 sequence data.

Jürgenstein S, Kurina O, Põldmaa K - Zookeys (2015)

Ventral branch of gonostylus, ventral view (15, 17, 19) and internal view (16, 18, 20). 15, 16Mycetophilabritannica17, 18Mycetophilaidonea19, 20Mycetophilaichneumonea. Scale bar = 0.05 mm. Abbreviations: pp = posterior process; sp = posterior spines on the ventral branch of gonostylus.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492204&req=5

Figure 5: Ventral branch of gonostylus, ventral view (15, 17, 19) and internal view (16, 18, 20). 15, 16Mycetophilabritannica17, 18Mycetophilaidonea19, 20Mycetophilaichneumonea. Scale bar = 0.05 mm. Abbreviations: pp = posterior process; sp = posterior spines on the ventral branch of gonostylus.
Mentions: Terminology used for describing male terminalia with synonyms from earlier studies and references to corresponding figures.

Bottom Line: European species of the Mycetophilaruficollis group are compared on the basis of morphology and sequences of mitochondrial cytochrome oxidase subunit one (COI) and the ITS2 region of nuclear ribosomal DNA.New country records, viz.Estonia for Mycetophilaevanida, Georgia for Mycetophilaichneumonea, Mycetophilaidonea and Mycetophilaruficollis, and Norway for Mycetophilastrobli, widen the known distribution ranges of these species.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi st 5D, 51014 Tartu, ESTONIA.

ABSTRACT
European species of the Mycetophilaruficollis group are compared on the basis of morphology and sequences of mitochondrial cytochrome oxidase subunit one (COI) and the ITS2 region of nuclear ribosomal DNA. The study represents the first evaluation of morphology-based species delimitation of closely related fungus gnat species by applying molecular information. Detailed descriptions and illustrations of the male terminalia are presented along with a key for the identification of all nine European species of the group. Phylogenetic analyses of molecular data generally supported the morphological species discrimination. The barcoding region of COI superseded ITS2 rDNA in resolving species. In the COI barcoding region interspecific differences ranged from 2.9 to 10.6% and the intraspecific distance from 0.08 to 0.8%. Only COI data distinguished between the similar and closely related Mycetophilaichneumonea and Mycetophilauninotata of which the latter was observed to include cryptic species. The host range of some species is suggested to be narrower than previously considered and to depend on the forest type. Presented evidence indicates the importance of analysing sequence data of morphologically very similar mycetophages reared from identified host fungi for elucidating species delimitation as well as their geographic and host ranges. New country records, viz. Estonia for Mycetophilaevanida, Georgia for Mycetophilaichneumonea, Mycetophilaidonea and Mycetophilaruficollis, and Norway for Mycetophilastrobli, widen the known distribution ranges of these species.

No MeSH data available.