Limits...
A Translational Animal Model for Scar Compression Therapy Using an Automated Pressure Delivery System.

Alkhalil A, Tejiram S, Travis TE, Prindeze NJ, Carney BC, Moffatt LT, Johnson LS, Ramella-Roman J, Shupp JW - Eplasty (2015)

Bottom Line: Gross scar examination by the Vancouver Scar Scale showed significant and sustained (>4 weeks) improvement in pressure-treated scars (P < .05).Histological examination of pressure-treated scars showed a significant decrease in dermal thickness compared with other groups (P < .05).Cellular quantification showed differential changes among treatment groups.

View Article: PubMed Central - PubMed

Affiliation: Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC.

ABSTRACT

Background: Pressure therapy has been used to prevent and treat hypertrophic scars following cutaneous injury despite the limited understanding of its mechanism of action and lack of established animal model to optimize its usage.

Objectives: The aim of this work was to test and characterize a novel automated pressure delivery system designed to deliver steady and controllable pressure in a red Duroc swine hypertrophic scar model.

Methods: Excisional wounds were created by dermatome on 6 red Duroc pigs and allowed to scar while assessed weekly via gross visual inspection, laser Doppler imaging, and biopsy. A portable novel automated pressure delivery system was mounted on developing scars (n = 6) for 2 weeks.

Results: The device maintained a pressure range of 30 ± 4 mm Hg for more than 90% of the 2-week treatment period. Pressure readings outside this designated range were attributed to normal animal behavior and responses to healing progression. Gross scar examination by the Vancouver Scar Scale showed significant and sustained (>4 weeks) improvement in pressure-treated scars (P < .05). Histological examination of pressure-treated scars showed a significant decrease in dermal thickness compared with other groups (P < .05). Pressure-treated scars also showed increased perfusion by laser Doppler imaging during the treatment period compared with sham-treated and untreated scars (P < .05). Cellular quantification showed differential changes among treatment groups.

Conclusion: These results illustrate the applications of this technology in hypertrophic scar Duroc swine model and the evaluation and optimization of pressure therapy in wound-healing and hypertrophic scar management.

No MeSH data available.


Related in: MedlinePlus

Comparison of laser Doppler imaging results between different treatment modalities during and after the compression period. Row images of pressure-treated, sham-treated, and untreated scars at days 70 and 84 (a), temporal perfusion changes of days 63 and 112 for pressure-treated and sham-treated scars (b). The inset shows perfusion 2 hours after removal of the automated pressure delivery system at day 84.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4492193&req=5

Figure 8: Comparison of laser Doppler imaging results between different treatment modalities during and after the compression period. Row images of pressure-treated, sham-treated, and untreated scars at days 70 and 84 (a), temporal perfusion changes of days 63 and 112 for pressure-treated and sham-treated scars (b). The inset shows perfusion 2 hours after removal of the automated pressure delivery system at day 84.

Mentions: Evaluation of wound perfusion before and after APDS mounting using LDI showed differences between pressure-treated scar and other arms of the study. While sham-treated and untreated scar produced laser Doppler images suggestive of no significant change, pressure-treated scar laser Doppler images showed evidence of increased scar perfusion by day 84 (Fig 8a). Further software-aided analysis confirmed a significant increase in scar perfusion (Fig 8b) relative to sham-treated and untreated scars during the treatment period.


A Translational Animal Model for Scar Compression Therapy Using an Automated Pressure Delivery System.

Alkhalil A, Tejiram S, Travis TE, Prindeze NJ, Carney BC, Moffatt LT, Johnson LS, Ramella-Roman J, Shupp JW - Eplasty (2015)

Comparison of laser Doppler imaging results between different treatment modalities during and after the compression period. Row images of pressure-treated, sham-treated, and untreated scars at days 70 and 84 (a), temporal perfusion changes of days 63 and 112 for pressure-treated and sham-treated scars (b). The inset shows perfusion 2 hours after removal of the automated pressure delivery system at day 84.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492193&req=5

Figure 8: Comparison of laser Doppler imaging results between different treatment modalities during and after the compression period. Row images of pressure-treated, sham-treated, and untreated scars at days 70 and 84 (a), temporal perfusion changes of days 63 and 112 for pressure-treated and sham-treated scars (b). The inset shows perfusion 2 hours after removal of the automated pressure delivery system at day 84.
Mentions: Evaluation of wound perfusion before and after APDS mounting using LDI showed differences between pressure-treated scar and other arms of the study. While sham-treated and untreated scar produced laser Doppler images suggestive of no significant change, pressure-treated scar laser Doppler images showed evidence of increased scar perfusion by day 84 (Fig 8a). Further software-aided analysis confirmed a significant increase in scar perfusion (Fig 8b) relative to sham-treated and untreated scars during the treatment period.

Bottom Line: Gross scar examination by the Vancouver Scar Scale showed significant and sustained (>4 weeks) improvement in pressure-treated scars (P < .05).Histological examination of pressure-treated scars showed a significant decrease in dermal thickness compared with other groups (P < .05).Cellular quantification showed differential changes among treatment groups.

View Article: PubMed Central - PubMed

Affiliation: Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC.

ABSTRACT

Background: Pressure therapy has been used to prevent and treat hypertrophic scars following cutaneous injury despite the limited understanding of its mechanism of action and lack of established animal model to optimize its usage.

Objectives: The aim of this work was to test and characterize a novel automated pressure delivery system designed to deliver steady and controllable pressure in a red Duroc swine hypertrophic scar model.

Methods: Excisional wounds were created by dermatome on 6 red Duroc pigs and allowed to scar while assessed weekly via gross visual inspection, laser Doppler imaging, and biopsy. A portable novel automated pressure delivery system was mounted on developing scars (n = 6) for 2 weeks.

Results: The device maintained a pressure range of 30 ± 4 mm Hg for more than 90% of the 2-week treatment period. Pressure readings outside this designated range were attributed to normal animal behavior and responses to healing progression. Gross scar examination by the Vancouver Scar Scale showed significant and sustained (>4 weeks) improvement in pressure-treated scars (P < .05). Histological examination of pressure-treated scars showed a significant decrease in dermal thickness compared with other groups (P < .05). Pressure-treated scars also showed increased perfusion by laser Doppler imaging during the treatment period compared with sham-treated and untreated scars (P < .05). Cellular quantification showed differential changes among treatment groups.

Conclusion: These results illustrate the applications of this technology in hypertrophic scar Duroc swine model and the evaluation and optimization of pressure therapy in wound-healing and hypertrophic scar management.

No MeSH data available.


Related in: MedlinePlus