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A Translational Animal Model for Scar Compression Therapy Using an Automated Pressure Delivery System.

Alkhalil A, Tejiram S, Travis TE, Prindeze NJ, Carney BC, Moffatt LT, Johnson LS, Ramella-Roman J, Shupp JW - Eplasty (2015)

Bottom Line: Pressure readings outside this designated range were attributed to normal animal behavior and responses to healing progression.Histological examination of pressure-treated scars showed a significant decrease in dermal thickness compared with other groups (P < .05).Cellular quantification showed differential changes among treatment groups.

View Article: PubMed Central - PubMed

Affiliation: Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC.

ABSTRACT

Background: Pressure therapy has been used to prevent and treat hypertrophic scars following cutaneous injury despite the limited understanding of its mechanism of action and lack of established animal model to optimize its usage.

Objectives: The aim of this work was to test and characterize a novel automated pressure delivery system designed to deliver steady and controllable pressure in a red Duroc swine hypertrophic scar model.

Methods: Excisional wounds were created by dermatome on 6 red Duroc pigs and allowed to scar while assessed weekly via gross visual inspection, laser Doppler imaging, and biopsy. A portable novel automated pressure delivery system was mounted on developing scars (n = 6) for 2 weeks.

Results: The device maintained a pressure range of 30 ± 4 mm Hg for more than 90% of the 2-week treatment period. Pressure readings outside this designated range were attributed to normal animal behavior and responses to healing progression. Gross scar examination by the Vancouver Scar Scale showed significant and sustained (>4 weeks) improvement in pressure-treated scars (P < .05). Histological examination of pressure-treated scars showed a significant decrease in dermal thickness compared with other groups (P < .05). Pressure-treated scars also showed increased perfusion by laser Doppler imaging during the treatment period compared with sham-treated and untreated scars (P < .05). Cellular quantification showed differential changes among treatment groups.

Conclusion: These results illustrate the applications of this technology in hypertrophic scar Duroc swine model and the evaluation and optimization of pressure therapy in wound-healing and hypertrophic scar management.

No MeSH data available.


Related in: MedlinePlus

Sections of biopsy specimens from pressure, sham, and no treatment scars at days 70 and 84 (a). Average percent cellularity per HPF compared on the basis of treatment (b). HPF indicates high-powered field. *Denotes statistical significance between treatment modalities.
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Figure 7: Sections of biopsy specimens from pressure, sham, and no treatment scars at days 70 and 84 (a). Average percent cellularity per HPF compared on the basis of treatment (b). HPF indicates high-powered field. *Denotes statistical significance between treatment modalities.

Mentions: H&E-stained sections from pressure-treated, sham-treated, and untreated HTS biopsy specimens were examined under the microscope for quantifiable cellular changes during and after the treatment period (Fig 7a) with results confirmed by DAPI fluorescent staining (not shown). Pressure-treated scars had a significant decrease in cellularity compared with both sham-treated and untreated scars 1 week into the treatment period (P < .05). However, cellularity increased significantly compared with its previous week as well as to sham-treated scars at day 84 and afterward (P < .05). Compared with untreated scars, pressure-treated scars had significantly lower cell counts after 1 week of treatment and up to 1 week after treatment (P < .05; Fig 7b).


A Translational Animal Model for Scar Compression Therapy Using an Automated Pressure Delivery System.

Alkhalil A, Tejiram S, Travis TE, Prindeze NJ, Carney BC, Moffatt LT, Johnson LS, Ramella-Roman J, Shupp JW - Eplasty (2015)

Sections of biopsy specimens from pressure, sham, and no treatment scars at days 70 and 84 (a). Average percent cellularity per HPF compared on the basis of treatment (b). HPF indicates high-powered field. *Denotes statistical significance between treatment modalities.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492193&req=5

Figure 7: Sections of biopsy specimens from pressure, sham, and no treatment scars at days 70 and 84 (a). Average percent cellularity per HPF compared on the basis of treatment (b). HPF indicates high-powered field. *Denotes statistical significance between treatment modalities.
Mentions: H&E-stained sections from pressure-treated, sham-treated, and untreated HTS biopsy specimens were examined under the microscope for quantifiable cellular changes during and after the treatment period (Fig 7a) with results confirmed by DAPI fluorescent staining (not shown). Pressure-treated scars had a significant decrease in cellularity compared with both sham-treated and untreated scars 1 week into the treatment period (P < .05). However, cellularity increased significantly compared with its previous week as well as to sham-treated scars at day 84 and afterward (P < .05). Compared with untreated scars, pressure-treated scars had significantly lower cell counts after 1 week of treatment and up to 1 week after treatment (P < .05; Fig 7b).

Bottom Line: Pressure readings outside this designated range were attributed to normal animal behavior and responses to healing progression.Histological examination of pressure-treated scars showed a significant decrease in dermal thickness compared with other groups (P < .05).Cellular quantification showed differential changes among treatment groups.

View Article: PubMed Central - PubMed

Affiliation: Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC.

ABSTRACT

Background: Pressure therapy has been used to prevent and treat hypertrophic scars following cutaneous injury despite the limited understanding of its mechanism of action and lack of established animal model to optimize its usage.

Objectives: The aim of this work was to test and characterize a novel automated pressure delivery system designed to deliver steady and controllable pressure in a red Duroc swine hypertrophic scar model.

Methods: Excisional wounds were created by dermatome on 6 red Duroc pigs and allowed to scar while assessed weekly via gross visual inspection, laser Doppler imaging, and biopsy. A portable novel automated pressure delivery system was mounted on developing scars (n = 6) for 2 weeks.

Results: The device maintained a pressure range of 30 ± 4 mm Hg for more than 90% of the 2-week treatment period. Pressure readings outside this designated range were attributed to normal animal behavior and responses to healing progression. Gross scar examination by the Vancouver Scar Scale showed significant and sustained (>4 weeks) improvement in pressure-treated scars (P < .05). Histological examination of pressure-treated scars showed a significant decrease in dermal thickness compared with other groups (P < .05). Pressure-treated scars also showed increased perfusion by laser Doppler imaging during the treatment period compared with sham-treated and untreated scars (P < .05). Cellular quantification showed differential changes among treatment groups.

Conclusion: These results illustrate the applications of this technology in hypertrophic scar Duroc swine model and the evaluation and optimization of pressure therapy in wound-healing and hypertrophic scar management.

No MeSH data available.


Related in: MedlinePlus