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Detecting the frequency of aminoglycoside modifying enzyme encoding genes among clinical isolates of methicillin-resistant Staphylococcus aureus.

Shokravi Z, Mehrad L, Ramazani A - Bioimpacts (2015)

Bottom Line: Resistance to penicillin G, oxacilin, gentamicin, erythromycin, clindamycin, kanamycin, and tobramycin was found in 96.4%, 98.3%, 51.7%, 53.4%, 55.2%, 62% and 58.6% of the isolates, respectively.Nineteen isolates were not typeable.In conclusion, the aac (6')/aph (2'') was the most common aminoglycoside modifying enzyme gene and SCCmec type II and V were the most frequent types detected in hospital isolates, respectively.

View Article: PubMed Central - HTML - PubMed

Affiliation: Microbiology Department, Faculty of Basic Sciences, Islamic Azad University, Arak, Iran.

ABSTRACT

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) plays an important role in causing many serious nosocomial infections. In this study, the antimicrobial susceptibility and the frequency of aminoglycoside modifying enzyme encoding genes among clinical isolates of methicillin-resistant Staphylococcus aureus was investigated from two university hospitals of Zanjan province of Iran.

Methods: In this study, the antimicrobial susceptibility of MRSA isolates to various antibiotics was investigated by the disk diffusion method. Multiplex PCR assays were used for the determination of aminoglycoside modifying enzyme (AME) genes and staphylococcal cassette chromosome mec (SCCmec) types in MRSA strains.

Results: All 58 MRSA isolates were sensitive to vancomycin. Resistance to penicillin G, oxacilin, gentamicin, erythromycin, clindamycin, kanamycin, and tobramycin was found in 96.4%, 98.3%, 51.7%, 53.4%, 55.2%, 62% and 58.6% of the isolates, respectively. The most prevalent AME genes were aac(6')/aph(2'') (48.3 %) followed by ant(4)-Ia (24%). The aph(3')-Ia gene was the least frequent AME gene among MRSA isolates (19%). Of the 58 tested MRSA isolates, 5 (8.6%) were harboured SCCmec type I, 11 (19%) SCCmec type II, 20 (34.5%) SCCmec type III, 17 (29.3%) SCCmec type IVa, 1 (1.7%) SCCmec type IVb, 2 (3.4%) SCCmec type IVc, 11 (19%) SCCmec type IVd, and, 18 (31%) SCCmec type V. Nineteen isolates were not typeable.

Conclusion: In conclusion, the aac (6')/aph (2'') was the most common aminoglycoside modifying enzyme gene and SCCmec type II and V were the most frequent types detected in hospital isolates, respectively.

No MeSH data available.


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Mentions: Among the 260 nasal swabs obtained from HCW, 104 of the isolates were identified as S. aureus through biochemical procedures. All the 104 isolates were Sa442 and femA positive (implying they were S. aureus), and the mecA gene (encoding PBP2a, a penicillin-binding protein that mediate meticillin resistance) was found in 58 of the 104 staphylococcal isolates based on PCR analysis (Fig. 1).7 These high rates of MRSA isolates are comparable with other reports that show similar rates.14


Detecting the frequency of aminoglycoside modifying enzyme encoding genes among clinical isolates of methicillin-resistant Staphylococcus aureus.

Shokravi Z, Mehrad L, Ramazani A - Bioimpacts (2015)

© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492189&req=5

Mentions: Among the 260 nasal swabs obtained from HCW, 104 of the isolates were identified as S. aureus through biochemical procedures. All the 104 isolates were Sa442 and femA positive (implying they were S. aureus), and the mecA gene (encoding PBP2a, a penicillin-binding protein that mediate meticillin resistance) was found in 58 of the 104 staphylococcal isolates based on PCR analysis (Fig. 1).7 These high rates of MRSA isolates are comparable with other reports that show similar rates.14

Bottom Line: Resistance to penicillin G, oxacilin, gentamicin, erythromycin, clindamycin, kanamycin, and tobramycin was found in 96.4%, 98.3%, 51.7%, 53.4%, 55.2%, 62% and 58.6% of the isolates, respectively.Nineteen isolates were not typeable.In conclusion, the aac (6')/aph (2'') was the most common aminoglycoside modifying enzyme gene and SCCmec type II and V were the most frequent types detected in hospital isolates, respectively.

View Article: PubMed Central - HTML - PubMed

Affiliation: Microbiology Department, Faculty of Basic Sciences, Islamic Azad University, Arak, Iran.

ABSTRACT

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) plays an important role in causing many serious nosocomial infections. In this study, the antimicrobial susceptibility and the frequency of aminoglycoside modifying enzyme encoding genes among clinical isolates of methicillin-resistant Staphylococcus aureus was investigated from two university hospitals of Zanjan province of Iran.

Methods: In this study, the antimicrobial susceptibility of MRSA isolates to various antibiotics was investigated by the disk diffusion method. Multiplex PCR assays were used for the determination of aminoglycoside modifying enzyme (AME) genes and staphylococcal cassette chromosome mec (SCCmec) types in MRSA strains.

Results: All 58 MRSA isolates were sensitive to vancomycin. Resistance to penicillin G, oxacilin, gentamicin, erythromycin, clindamycin, kanamycin, and tobramycin was found in 96.4%, 98.3%, 51.7%, 53.4%, 55.2%, 62% and 58.6% of the isolates, respectively. The most prevalent AME genes were aac(6')/aph(2'') (48.3 %) followed by ant(4)-Ia (24%). The aph(3')-Ia gene was the least frequent AME gene among MRSA isolates (19%). Of the 58 tested MRSA isolates, 5 (8.6%) were harboured SCCmec type I, 11 (19%) SCCmec type II, 20 (34.5%) SCCmec type III, 17 (29.3%) SCCmec type IVa, 1 (1.7%) SCCmec type IVb, 2 (3.4%) SCCmec type IVc, 11 (19%) SCCmec type IVd, and, 18 (31%) SCCmec type V. Nineteen isolates were not typeable.

Conclusion: In conclusion, the aac (6')/aph (2'') was the most common aminoglycoside modifying enzyme gene and SCCmec type II and V were the most frequent types detected in hospital isolates, respectively.

No MeSH data available.


Related in: MedlinePlus