Limits...
Antioxidants in different parts of oleaster as a function of genotype.

Faramarz S, Dehghan G, Jahanban-Esfahlan A - Bioimpacts (2015)

Bottom Line: The phenol and flavonoid contents were determined using spectrophotometric methods.Antioxidant and antiradical activities were determined using reducing power, ferric-reducing antioxidant potential (FRAP) and ability to scavenge DPPH radical assays.Seeds of fruits have excellent antioxidant activity and phenolic contents in comparison to flesh and peel.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Plant Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.

ABSTRACT

Introduction: Fruits of oleaster (Elaeagnus angustifolia L.) were used in traditional medicine to treat various diseases. The aim of this study was to evaluate and compare the phenol and flavonoid contents and antioxidant activity of methanol extracts from the fruit peel, flesh and seed of seven genotypes of oleaster.

Methods: The phenol and flavonoid contents were determined using spectrophotometric methods. Antioxidant and antiradical activities were determined using reducing power, ferric-reducing antioxidant potential (FRAP) and ability to scavenge DPPH radical assays.

Results: Significant differences ( P < 0.05) were found in phenol and flavonoid contents and antioxidant activity among components of fruit and within various genotypes.

Conclusion: RESULTS indicated that oleaster has good fruit quality varying among different genotypes. Seeds of fruits have excellent antioxidant activity and phenolic contents in comparison to flesh and peel.

No MeSH data available.


Related in: MedlinePlus

© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4492188&req=5

Mentions: Radical scavenging activity of the extracts was determined through the reduction in the optical absorbance at 517 nm due to scavenging of the stable DPPH free radical. A positive DPPH test suggests that the samples are free radical scavengers. The radical scavenging activities of samples were calculated against the blank solution and samples showed various results that are presented in Fig. 5. The inhibition percentage of all extracts on the DPPH radical was found to be dependent on the concentration. It was shown that seed was stronger in the scavenging of DPPH radical as compared to other components. In order to quantify the antioxidant activity, IC50 was further calculated (Table 1). The IC50 is the concentration of sample required to decrease the absorbance at 517 nm by 50%. A lower value of IC50 would reflect greater antioxidant activity of the sample. In the current study, the highest radical scavenging activity was observed in the seed extract of IEa-3 (IC50 = 24.5 mg/mL), whereas the methanol extract of peel of IEa-6 showed lower activity (IC50 = 123.14 mg/mL).


Antioxidants in different parts of oleaster as a function of genotype.

Faramarz S, Dehghan G, Jahanban-Esfahlan A - Bioimpacts (2015)

© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492188&req=5

Mentions: Radical scavenging activity of the extracts was determined through the reduction in the optical absorbance at 517 nm due to scavenging of the stable DPPH free radical. A positive DPPH test suggests that the samples are free radical scavengers. The radical scavenging activities of samples were calculated against the blank solution and samples showed various results that are presented in Fig. 5. The inhibition percentage of all extracts on the DPPH radical was found to be dependent on the concentration. It was shown that seed was stronger in the scavenging of DPPH radical as compared to other components. In order to quantify the antioxidant activity, IC50 was further calculated (Table 1). The IC50 is the concentration of sample required to decrease the absorbance at 517 nm by 50%. A lower value of IC50 would reflect greater antioxidant activity of the sample. In the current study, the highest radical scavenging activity was observed in the seed extract of IEa-3 (IC50 = 24.5 mg/mL), whereas the methanol extract of peel of IEa-6 showed lower activity (IC50 = 123.14 mg/mL).

Bottom Line: The phenol and flavonoid contents were determined using spectrophotometric methods.Antioxidant and antiradical activities were determined using reducing power, ferric-reducing antioxidant potential (FRAP) and ability to scavenge DPPH radical assays.Seeds of fruits have excellent antioxidant activity and phenolic contents in comparison to flesh and peel.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Plant Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.

ABSTRACT

Introduction: Fruits of oleaster (Elaeagnus angustifolia L.) were used in traditional medicine to treat various diseases. The aim of this study was to evaluate and compare the phenol and flavonoid contents and antioxidant activity of methanol extracts from the fruit peel, flesh and seed of seven genotypes of oleaster.

Methods: The phenol and flavonoid contents were determined using spectrophotometric methods. Antioxidant and antiradical activities were determined using reducing power, ferric-reducing antioxidant potential (FRAP) and ability to scavenge DPPH radical assays.

Results: Significant differences ( P < 0.05) were found in phenol and flavonoid contents and antioxidant activity among components of fruit and within various genotypes.

Conclusion: RESULTS indicated that oleaster has good fruit quality varying among different genotypes. Seeds of fruits have excellent antioxidant activity and phenolic contents in comparison to flesh and peel.

No MeSH data available.


Related in: MedlinePlus