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Isolation and characterization of Borrelia burgdorferi strains from Ixodes ricinus ticks in the southern England.

Sorouri R, Ramazani A, Karami A, Ranjbar R, Guy EC - Bioimpacts (2015)

Bottom Line: The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates.These studies identified six protein antigens with molecular weights of 18, 30, 39, 47, 60 and 88 kDa with particular promise for detecting specific immune responses to B. burgdorferi infection including Lyme disease.As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Introduction: Lyme disease is a bacterial infection caused by the spiral-shaped bacterium Borrelia burgdorferi. We investigated the presence and prevalence of Borrelia species in ticks from the southern England.

Methods: One hundred fifty-five cases (103 adult and 52 nymphal ticks) were collected from animal carcases. The midguts were removed, cultured in Barbour/Stoenner/Kelly II (BSK-II) and Barbour/ Stoenner/Kelly F (BSK-F) media and examined by IF, dark-field microscopy, and nested PCR.

Results: From a total 155 cultured ticks, two showed evidence of spirochetes and denoted as SO-1 and SO-2 strains. The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates. These studies identified six protein antigens with molecular weights of 18, 30, 39, 47, 60 and 88 kDa with particular promise for detecting specific immune responses to B. burgdorferi infection including Lyme disease. We also investigated the effect of repeated subculture on the antigenic pattern of UK isolate of B. burgdorferi.

Conclusion: As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

No MeSH data available.


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Mentions: The results shown in Fig. 3 reveal considerable antigenic similarities between the UK and the standard strains. In each case reaction could be seen with the major bands observed on SDS-PAGE (except 31 KDa band), namely the 21/22, 41, and 60/6l kDa bands. One serum reacted with the putative OSP-B from SO-2 and ACA-1, but not with SO-1 and B-31. The second serum failed to react with any of these bands. In addition, immunological reactivity was also seen with 30 and 39 kDa bands which were not readily detected on SDS-PAGE. Variable reaction was seen with a band of 18 kDa which was only weakly visible on staining of the strains SO-1 and SO-2 and not ACA-1 and B-31.


Isolation and characterization of Borrelia burgdorferi strains from Ixodes ricinus ticks in the southern England.

Sorouri R, Ramazani A, Karami A, Ranjbar R, Guy EC - Bioimpacts (2015)

© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492187&req=5

Mentions: The results shown in Fig. 3 reveal considerable antigenic similarities between the UK and the standard strains. In each case reaction could be seen with the major bands observed on SDS-PAGE (except 31 KDa band), namely the 21/22, 41, and 60/6l kDa bands. One serum reacted with the putative OSP-B from SO-2 and ACA-1, but not with SO-1 and B-31. The second serum failed to react with any of these bands. In addition, immunological reactivity was also seen with 30 and 39 kDa bands which were not readily detected on SDS-PAGE. Variable reaction was seen with a band of 18 kDa which was only weakly visible on staining of the strains SO-1 and SO-2 and not ACA-1 and B-31.

Bottom Line: The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates.These studies identified six protein antigens with molecular weights of 18, 30, 39, 47, 60 and 88 kDa with particular promise for detecting specific immune responses to B. burgdorferi infection including Lyme disease.As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Introduction: Lyme disease is a bacterial infection caused by the spiral-shaped bacterium Borrelia burgdorferi. We investigated the presence and prevalence of Borrelia species in ticks from the southern England.

Methods: One hundred fifty-five cases (103 adult and 52 nymphal ticks) were collected from animal carcases. The midguts were removed, cultured in Barbour/Stoenner/Kelly II (BSK-II) and Barbour/ Stoenner/Kelly F (BSK-F) media and examined by IF, dark-field microscopy, and nested PCR.

Results: From a total 155 cultured ticks, two showed evidence of spirochetes and denoted as SO-1 and SO-2 strains. The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates. These studies identified six protein antigens with molecular weights of 18, 30, 39, 47, 60 and 88 kDa with particular promise for detecting specific immune responses to B. burgdorferi infection including Lyme disease. We also investigated the effect of repeated subculture on the antigenic pattern of UK isolate of B. burgdorferi.

Conclusion: As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

No MeSH data available.


Related in: MedlinePlus