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Isolation and characterization of Borrelia burgdorferi strains from Ixodes ricinus ticks in the southern England.

Sorouri R, Ramazani A, Karami A, Ranjbar R, Guy EC - Bioimpacts (2015)

Bottom Line: From a total 155 cultured ticks, two showed evidence of spirochetes and denoted as SO-1 and SO-2 strains.The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates.As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Introduction: Lyme disease is a bacterial infection caused by the spiral-shaped bacterium Borrelia burgdorferi. We investigated the presence and prevalence of Borrelia species in ticks from the southern England.

Methods: One hundred fifty-five cases (103 adult and 52 nymphal ticks) were collected from animal carcases. The midguts were removed, cultured in Barbour/Stoenner/Kelly II (BSK-II) and Barbour/ Stoenner/Kelly F (BSK-F) media and examined by IF, dark-field microscopy, and nested PCR.

Results: From a total 155 cultured ticks, two showed evidence of spirochetes and denoted as SO-1 and SO-2 strains. The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates. These studies identified six protein antigens with molecular weights of 18, 30, 39, 47, 60 and 88 kDa with particular promise for detecting specific immune responses to B. burgdorferi infection including Lyme disease. We also investigated the effect of repeated subculture on the antigenic pattern of UK isolate of B. burgdorferi.

Conclusion: As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

No MeSH data available.


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Mentions: SDS-PAGE was used to compare strains isolated from different sources.23 Two UK strains were therefore subjected to SDS-PAGE alongside control strains ACA-1 (Sweden) and B-31 (USA). The protein profile after staining with PAGE Blue is shown in Fig. 2. The gel profiles showed more similarity along with some significant differences, particularly in the major proteins (Table 4). The major differences seen occurred in the region 32- 35 kDa characteristic of the OSP-B. Strain SO-2 had a major band at 35 kDa similar to the presumed OSP-B bands in strains ACA-1 (35 kDa) and B-31 (33 kDa), while strain SO-1 had only a weak band at approximately 32 kDa. Both isolates had a major band at approximately 31 kDa characteristic of the OSP-A, although the band in SO-2 appeared to be slightly lower in molecular weight. Strains SO-1 and SO-2, in common with the vast majority of other strains of B. burgdorferi, had major proteins of 4l kDa and 60 kDa molecular sizes corresponding to flagellin and common antigen, respectively.24-26 One major difference observed was the presence of a major band at 21-22 kDa in the UK isolates which was not apparent in the standard strains.


Isolation and characterization of Borrelia burgdorferi strains from Ixodes ricinus ticks in the southern England.

Sorouri R, Ramazani A, Karami A, Ranjbar R, Guy EC - Bioimpacts (2015)

© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492187&req=5

Mentions: SDS-PAGE was used to compare strains isolated from different sources.23 Two UK strains were therefore subjected to SDS-PAGE alongside control strains ACA-1 (Sweden) and B-31 (USA). The protein profile after staining with PAGE Blue is shown in Fig. 2. The gel profiles showed more similarity along with some significant differences, particularly in the major proteins (Table 4). The major differences seen occurred in the region 32- 35 kDa characteristic of the OSP-B. Strain SO-2 had a major band at 35 kDa similar to the presumed OSP-B bands in strains ACA-1 (35 kDa) and B-31 (33 kDa), while strain SO-1 had only a weak band at approximately 32 kDa. Both isolates had a major band at approximately 31 kDa characteristic of the OSP-A, although the band in SO-2 appeared to be slightly lower in molecular weight. Strains SO-1 and SO-2, in common with the vast majority of other strains of B. burgdorferi, had major proteins of 4l kDa and 60 kDa molecular sizes corresponding to flagellin and common antigen, respectively.24-26 One major difference observed was the presence of a major band at 21-22 kDa in the UK isolates which was not apparent in the standard strains.

Bottom Line: From a total 155 cultured ticks, two showed evidence of spirochetes and denoted as SO-1 and SO-2 strains.The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates.As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Introduction: Lyme disease is a bacterial infection caused by the spiral-shaped bacterium Borrelia burgdorferi. We investigated the presence and prevalence of Borrelia species in ticks from the southern England.

Methods: One hundred fifty-five cases (103 adult and 52 nymphal ticks) were collected from animal carcases. The midguts were removed, cultured in Barbour/Stoenner/Kelly II (BSK-II) and Barbour/ Stoenner/Kelly F (BSK-F) media and examined by IF, dark-field microscopy, and nested PCR.

Results: From a total 155 cultured ticks, two showed evidence of spirochetes and denoted as SO-1 and SO-2 strains. The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates. These studies identified six protein antigens with molecular weights of 18, 30, 39, 47, 60 and 88 kDa with particular promise for detecting specific immune responses to B. burgdorferi infection including Lyme disease. We also investigated the effect of repeated subculture on the antigenic pattern of UK isolate of B. burgdorferi.

Conclusion: As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

No MeSH data available.


Related in: MedlinePlus