Limits...
Isolation and characterization of Borrelia burgdorferi strains from Ixodes ricinus ticks in the southern England.

Sorouri R, Ramazani A, Karami A, Ranjbar R, Guy EC - Bioimpacts (2015)

Bottom Line: The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates.These studies identified six protein antigens with molecular weights of 18, 30, 39, 47, 60 and 88 kDa with particular promise for detecting specific immune responses to B. burgdorferi infection including Lyme disease.As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Introduction: Lyme disease is a bacterial infection caused by the spiral-shaped bacterium Borrelia burgdorferi. We investigated the presence and prevalence of Borrelia species in ticks from the southern England.

Methods: One hundred fifty-five cases (103 adult and 52 nymphal ticks) were collected from animal carcases. The midguts were removed, cultured in Barbour/Stoenner/Kelly II (BSK-II) and Barbour/ Stoenner/Kelly F (BSK-F) media and examined by IF, dark-field microscopy, and nested PCR.

Results: From a total 155 cultured ticks, two showed evidence of spirochetes and denoted as SO-1 and SO-2 strains. The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates. These studies identified six protein antigens with molecular weights of 18, 30, 39, 47, 60 and 88 kDa with particular promise for detecting specific immune responses to B. burgdorferi infection including Lyme disease. We also investigated the effect of repeated subculture on the antigenic pattern of UK isolate of B. burgdorferi.

Conclusion: As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

No MeSH data available.


Related in: MedlinePlus

© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4492187&req=5

Mentions: A total of 103 adult ticks from Savernake Forest, Harewood, Great Ridge, Grovely Wood, and 52 groups of nymphal ticks from all locations (except Eyeworth plain and Richmond Park) were tested using the IF method. The midgut contents of two adult ticks, one from Grovely Wood and one from Savernake Forest, showed positive IF (Fig. 1) with the polyclonal antiserum but not with the monoclonal antibodies. In addition, one group of macerated nymphal ticks from Battery Hill showed a weak positive result with the polyclonal antiserum.


Isolation and characterization of Borrelia burgdorferi strains from Ixodes ricinus ticks in the southern England.

Sorouri R, Ramazani A, Karami A, Ranjbar R, Guy EC - Bioimpacts (2015)

© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492187&req=5

Mentions: A total of 103 adult ticks from Savernake Forest, Harewood, Great Ridge, Grovely Wood, and 52 groups of nymphal ticks from all locations (except Eyeworth plain and Richmond Park) were tested using the IF method. The midgut contents of two adult ticks, one from Grovely Wood and one from Savernake Forest, showed positive IF (Fig. 1) with the polyclonal antiserum but not with the monoclonal antibodies. In addition, one group of macerated nymphal ticks from Battery Hill showed a weak positive result with the polyclonal antiserum.

Bottom Line: The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates.These studies identified six protein antigens with molecular weights of 18, 30, 39, 47, 60 and 88 kDa with particular promise for detecting specific immune responses to B. burgdorferi infection including Lyme disease.As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.

ABSTRACT

Introduction: Lyme disease is a bacterial infection caused by the spiral-shaped bacterium Borrelia burgdorferi. We investigated the presence and prevalence of Borrelia species in ticks from the southern England.

Methods: One hundred fifty-five cases (103 adult and 52 nymphal ticks) were collected from animal carcases. The midguts were removed, cultured in Barbour/Stoenner/Kelly II (BSK-II) and Barbour/ Stoenner/Kelly F (BSK-F) media and examined by IF, dark-field microscopy, and nested PCR.

Results: From a total 155 cultured ticks, two showed evidence of spirochetes and denoted as SO-1 and SO-2 strains. The availability of these two isolates enabled their antigenic characterization with SDS-PAGE and western blotting and comparison with two standard isolates. These studies identified six protein antigens with molecular weights of 18, 30, 39, 47, 60 and 88 kDa with particular promise for detecting specific immune responses to B. burgdorferi infection including Lyme disease. We also investigated the effect of repeated subculture on the antigenic pattern of UK isolate of B. burgdorferi.

Conclusion: As a result of this study, antigenic differences have been seen between the UK isolates and the foreign isolates used as laboratory standards.

No MeSH data available.


Related in: MedlinePlus