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Transcription Factor T-Bet in Atlantic Salmon: Characterization and Gene Expression in Mucosal Tissues during Aeromonas Salmonicida Infection.

Kumari J, Zhang Z, Swain T, Chi H, Niu C, Bøgwald J, Dalmo RA - Front Immunol (2015)

Bottom Line: Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet.Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels.Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Nofima , Tromsø , Norway.

ABSTRACT
The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 bp). The putative primary structure of the polypeptide deduced from the cDNA sequence contained 612 aa, which possessed a T-box DNA binding domain. Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet. Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels. Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

No MeSH data available.


Related in: MedlinePlus

Tissue specific expression of T-bet, IL-22, NKEF, and IFNγ in infected Atlantic salmon at different time-points after water-borne infection with A. salmonicida. Bars represent the relative expression levels of T-bet, IL-22, NKEF, and IFNγ normalized to EF-1α. Each value represents the mean ± SEM (n = 4). Statistical differences (P < 0.05, P < 0.01, and P < 0.001) between different time-points compared to control are indicated by asterisk (*,**, and ***) respectively, above the bars.
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Figure 7: Tissue specific expression of T-bet, IL-22, NKEF, and IFNγ in infected Atlantic salmon at different time-points after water-borne infection with A. salmonicida. Bars represent the relative expression levels of T-bet, IL-22, NKEF, and IFNγ normalized to EF-1α. Each value represents the mean ± SEM (n = 4). Statistical differences (P < 0.05, P < 0.01, and P < 0.001) between different time-points compared to control are indicated by asterisk (*,**, and ***) respectively, above the bars.

Mentions: During water-borne infection with A. salmonicida, early (day 5) and strong T-bet expression in skin and head kidney was found (P < 0.05), whereas a moderate and delayed expression (P < 0.05) in the spleen (at day 10) and moderate to strong and also delayed expression (P < 0.05, 0.01) in the intestine (day 14 and 21) were observed, respectively (Figure 7). Similarly, NKEF expression was also significantly upregulated in all the tissues, especially in skin (P < 0.001) at all the time-points post challenge. This noticeable up-regulation of T-bet and NKEF at all the time-points in skin, points to an interesting co-regulation. Furthermore, IFNγ expression was, in general, also significantly upregulated in all the tissues of the co-habitants after 8–10 days of challenge except in the spleen. Alternatively, IL-22 showed a different response of strong significant expression only in intestine, suggesting the presence of ILC3-like subset that specifically secretes IL-22 during the response (may be T-bet regulated), similar to mammals. In spleen, head kidney, and skin, IL-22 expression level was similar to that of the control fish.


Transcription Factor T-Bet in Atlantic Salmon: Characterization and Gene Expression in Mucosal Tissues during Aeromonas Salmonicida Infection.

Kumari J, Zhang Z, Swain T, Chi H, Niu C, Bøgwald J, Dalmo RA - Front Immunol (2015)

Tissue specific expression of T-bet, IL-22, NKEF, and IFNγ in infected Atlantic salmon at different time-points after water-borne infection with A. salmonicida. Bars represent the relative expression levels of T-bet, IL-22, NKEF, and IFNγ normalized to EF-1α. Each value represents the mean ± SEM (n = 4). Statistical differences (P < 0.05, P < 0.01, and P < 0.001) between different time-points compared to control are indicated by asterisk (*,**, and ***) respectively, above the bars.
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4492157&req=5

Figure 7: Tissue specific expression of T-bet, IL-22, NKEF, and IFNγ in infected Atlantic salmon at different time-points after water-borne infection with A. salmonicida. Bars represent the relative expression levels of T-bet, IL-22, NKEF, and IFNγ normalized to EF-1α. Each value represents the mean ± SEM (n = 4). Statistical differences (P < 0.05, P < 0.01, and P < 0.001) between different time-points compared to control are indicated by asterisk (*,**, and ***) respectively, above the bars.
Mentions: During water-borne infection with A. salmonicida, early (day 5) and strong T-bet expression in skin and head kidney was found (P < 0.05), whereas a moderate and delayed expression (P < 0.05) in the spleen (at day 10) and moderate to strong and also delayed expression (P < 0.05, 0.01) in the intestine (day 14 and 21) were observed, respectively (Figure 7). Similarly, NKEF expression was also significantly upregulated in all the tissues, especially in skin (P < 0.001) at all the time-points post challenge. This noticeable up-regulation of T-bet and NKEF at all the time-points in skin, points to an interesting co-regulation. Furthermore, IFNγ expression was, in general, also significantly upregulated in all the tissues of the co-habitants after 8–10 days of challenge except in the spleen. Alternatively, IL-22 showed a different response of strong significant expression only in intestine, suggesting the presence of ILC3-like subset that specifically secretes IL-22 during the response (may be T-bet regulated), similar to mammals. In spleen, head kidney, and skin, IL-22 expression level was similar to that of the control fish.

Bottom Line: Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet.Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels.Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Nofima , Tromsø , Norway.

ABSTRACT
The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 bp). The putative primary structure of the polypeptide deduced from the cDNA sequence contained 612 aa, which possessed a T-box DNA binding domain. Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet. Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels. Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

No MeSH data available.


Related in: MedlinePlus