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Transcription Factor T-Bet in Atlantic Salmon: Characterization and Gene Expression in Mucosal Tissues during Aeromonas Salmonicida Infection.

Kumari J, Zhang Z, Swain T, Chi H, Niu C, Bøgwald J, Dalmo RA - Front Immunol (2015)

Bottom Line: Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet.Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels.Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Nofima , Tromsø , Norway.

ABSTRACT
The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 bp). The putative primary structure of the polypeptide deduced from the cDNA sequence contained 612 aa, which possessed a T-box DNA binding domain. Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet. Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels. Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

No MeSH data available.


Related in: MedlinePlus

Regulators of T-bet expression in salmon leukocytes. Spleen leukocytes were stimulated for 24, 48, and 72 h with ConA + PHA + huIL-2, IFN-α (0.5 mg/ml and 5 ng/ml), and the mRNA levels of T-bet were determined by real-time PCR. Gene expression is normalized against EF-1α and is shown relative to the mean of the non-stimulated cells (leukocyte cells with no treatment, i.e., only media control). Spleen leukocytes with no treatment were considered as control for each time points. Each bar represents the mean ± SE of triplicate samples. Different letters denote statistically significant differences (P < 0.05) between the groups.
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Figure 6: Regulators of T-bet expression in salmon leukocytes. Spleen leukocytes were stimulated for 24, 48, and 72 h with ConA + PHA + huIL-2, IFN-α (0.5 mg/ml and 5 ng/ml), and the mRNA levels of T-bet were determined by real-time PCR. Gene expression is normalized against EF-1α and is shown relative to the mean of the non-stimulated cells (leukocyte cells with no treatment, i.e., only media control). Spleen leukocytes with no treatment were considered as control for each time points. Each bar represents the mean ± SE of triplicate samples. Different letters denote statistically significant differences (P < 0.05) between the groups.

Mentions: Some stimulants known to activate lymphocytes were tested for their potency to regulate the expression of T-bet in spleen leukocytes. Interestingly, stimulation of spleen leukocytes with known T cell stimulants, i.e., PHA + ConA and rhIL-2 resulted in the strongest induction of T-bet expression starting from 24 h and it was maintained until 72 h of incubation. Stimulation with recombinant salmon IFN-α2 (0.5 μg/ml) also showed gradual and significant higher expression of T-bet from 48 h and onward compared to expression at 24 h (Figure 6).


Transcription Factor T-Bet in Atlantic Salmon: Characterization and Gene Expression in Mucosal Tissues during Aeromonas Salmonicida Infection.

Kumari J, Zhang Z, Swain T, Chi H, Niu C, Bøgwald J, Dalmo RA - Front Immunol (2015)

Regulators of T-bet expression in salmon leukocytes. Spleen leukocytes were stimulated for 24, 48, and 72 h with ConA + PHA + huIL-2, IFN-α (0.5 mg/ml and 5 ng/ml), and the mRNA levels of T-bet were determined by real-time PCR. Gene expression is normalized against EF-1α and is shown relative to the mean of the non-stimulated cells (leukocyte cells with no treatment, i.e., only media control). Spleen leukocytes with no treatment were considered as control for each time points. Each bar represents the mean ± SE of triplicate samples. Different letters denote statistically significant differences (P < 0.05) between the groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492157&req=5

Figure 6: Regulators of T-bet expression in salmon leukocytes. Spleen leukocytes were stimulated for 24, 48, and 72 h with ConA + PHA + huIL-2, IFN-α (0.5 mg/ml and 5 ng/ml), and the mRNA levels of T-bet were determined by real-time PCR. Gene expression is normalized against EF-1α and is shown relative to the mean of the non-stimulated cells (leukocyte cells with no treatment, i.e., only media control). Spleen leukocytes with no treatment were considered as control for each time points. Each bar represents the mean ± SE of triplicate samples. Different letters denote statistically significant differences (P < 0.05) between the groups.
Mentions: Some stimulants known to activate lymphocytes were tested for their potency to regulate the expression of T-bet in spleen leukocytes. Interestingly, stimulation of spleen leukocytes with known T cell stimulants, i.e., PHA + ConA and rhIL-2 resulted in the strongest induction of T-bet expression starting from 24 h and it was maintained until 72 h of incubation. Stimulation with recombinant salmon IFN-α2 (0.5 μg/ml) also showed gradual and significant higher expression of T-bet from 48 h and onward compared to expression at 24 h (Figure 6).

Bottom Line: Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet.Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels.Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Nofima , Tromsø , Norway.

ABSTRACT
The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 bp). The putative primary structure of the polypeptide deduced from the cDNA sequence contained 612 aa, which possessed a T-box DNA binding domain. Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet. Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels. Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

No MeSH data available.


Related in: MedlinePlus