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Transcription Factor T-Bet in Atlantic Salmon: Characterization and Gene Expression in Mucosal Tissues during Aeromonas Salmonicida Infection.

Kumari J, Zhang Z, Swain T, Chi H, Niu C, Bøgwald J, Dalmo RA - Front Immunol (2015)

Bottom Line: Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet.Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels.Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Nofima , Tromsø , Norway.

ABSTRACT
The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 bp). The putative primary structure of the polypeptide deduced from the cDNA sequence contained 612 aa, which possessed a T-box DNA binding domain. Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet. Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels. Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

No MeSH data available.


Related in: MedlinePlus

Tissue distribution of T-bet expression in healthy Atlantic salmon. Expression of salmon T-bet in different organs by real-time PCR. Gene expression data were normalized to EF-1α expression using liver as a calibrator. Bar represents the mean ± SEM (n = 6). Asterisk (*) above the bar shows significant difference (P < 0.05) compared with the organ that showed the lowest expression (liver). The value above the bars shows average real-time CT values of six fish.
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Figure 5: Tissue distribution of T-bet expression in healthy Atlantic salmon. Expression of salmon T-bet in different organs by real-time PCR. Gene expression data were normalized to EF-1α expression using liver as a calibrator. Bar represents the mean ± SEM (n = 6). Asterisk (*) above the bar shows significant difference (P < 0.05) compared with the organ that showed the lowest expression (liver). The value above the bars shows average real-time CT values of six fish.

Mentions: Quantitative PCR analysis showed that salmon T-bet gene was ubiquitously expressed in all the tissues analyzed, but was highly expressed in the spleen and head kidney showing about 100 and 50 times higher expression than that of intestine, skin, and ovary. Moderate transcript levels were observed in gill, brain, thymus, and testis, whereas weak expression was detected in the intestine, ovary, and skin with the lowest expression in the liver (Figure 5).


Transcription Factor T-Bet in Atlantic Salmon: Characterization and Gene Expression in Mucosal Tissues during Aeromonas Salmonicida Infection.

Kumari J, Zhang Z, Swain T, Chi H, Niu C, Bøgwald J, Dalmo RA - Front Immunol (2015)

Tissue distribution of T-bet expression in healthy Atlantic salmon. Expression of salmon T-bet in different organs by real-time PCR. Gene expression data were normalized to EF-1α expression using liver as a calibrator. Bar represents the mean ± SEM (n = 6). Asterisk (*) above the bar shows significant difference (P < 0.05) compared with the organ that showed the lowest expression (liver). The value above the bars shows average real-time CT values of six fish.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492157&req=5

Figure 5: Tissue distribution of T-bet expression in healthy Atlantic salmon. Expression of salmon T-bet in different organs by real-time PCR. Gene expression data were normalized to EF-1α expression using liver as a calibrator. Bar represents the mean ± SEM (n = 6). Asterisk (*) above the bar shows significant difference (P < 0.05) compared with the organ that showed the lowest expression (liver). The value above the bars shows average real-time CT values of six fish.
Mentions: Quantitative PCR analysis showed that salmon T-bet gene was ubiquitously expressed in all the tissues analyzed, but was highly expressed in the spleen and head kidney showing about 100 and 50 times higher expression than that of intestine, skin, and ovary. Moderate transcript levels were observed in gill, brain, thymus, and testis, whereas weak expression was detected in the intestine, ovary, and skin with the lowest expression in the liver (Figure 5).

Bottom Line: Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet.Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels.Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Nofima , Tromsø , Norway.

ABSTRACT
The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 bp). The putative primary structure of the polypeptide deduced from the cDNA sequence contained 612 aa, which possessed a T-box DNA binding domain. Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet. Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels. Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

No MeSH data available.


Related in: MedlinePlus