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Transcription Factor T-Bet in Atlantic Salmon: Characterization and Gene Expression in Mucosal Tissues during Aeromonas Salmonicida Infection.

Kumari J, Zhang Z, Swain T, Chi H, Niu C, Bøgwald J, Dalmo RA - Front Immunol (2015)

Bottom Line: Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet.Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels.Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Nofima , Tromsø , Norway.

ABSTRACT
The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 bp). The putative primary structure of the polypeptide deduced from the cDNA sequence contained 612 aa, which possessed a T-box DNA binding domain. Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet. Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels. Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

No MeSH data available.


Related in: MedlinePlus

Nucleotide and deduced amino acid sequence of Atlantic salmon T-bet cDNA. Uppercase denotes the UTR’s and lowercase denotes the coding regions. The T-box DNA binding domain is underlined. Start and stop codons are marked with bold letters. The asterisk indicates the stop codon. The putative polyadenylation signal is bold and underlined. A region of repeat with 23 copies of the consensus sequences CA (2 bp) is shaded.
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Figure 1: Nucleotide and deduced amino acid sequence of Atlantic salmon T-bet cDNA. Uppercase denotes the UTR’s and lowercase denotes the coding regions. The T-box DNA binding domain is underlined. Start and stop codons are marked with bold letters. The asterisk indicates the stop codon. The putative polyadenylation signal is bold and underlined. A region of repeat with 23 copies of the consensus sequences CA (2 bp) is shaded.

Mentions: To obtain full-length cDNA of AsT-bet, 5′ and 3′ RACE were performed using spleen derived cDNA as template. The Atlantic salmon T-bet cDNA sequence (GenBank accession no. GU979861) consisted of 3502 bp in length including 339 bp 5′ untranslated region (UTR), a 1324 bp 3′-UTR and possessed an open reading frame of 1839 bp (Figure 1). In the 3′-UTR, there is a polyadenylation signal (AATAAA) 21 bp upstream of poly-A tail and in addition, one region of repeat with 23 copies of the consensus sequence CA (2 bp) was also identified in the 3′-UTR using tandem repeats finder program (34). The putative T-bet protein in Atlantic salmon was predicted to be 612 aa long, with a calculated molecular weight of 67.8 kDa and a pI of 8.02. The conserved T-box DNA binding domain consisted of 196 aa.


Transcription Factor T-Bet in Atlantic Salmon: Characterization and Gene Expression in Mucosal Tissues during Aeromonas Salmonicida Infection.

Kumari J, Zhang Z, Swain T, Chi H, Niu C, Bøgwald J, Dalmo RA - Front Immunol (2015)

Nucleotide and deduced amino acid sequence of Atlantic salmon T-bet cDNA. Uppercase denotes the UTR’s and lowercase denotes the coding regions. The T-box DNA binding domain is underlined. Start and stop codons are marked with bold letters. The asterisk indicates the stop codon. The putative polyadenylation signal is bold and underlined. A region of repeat with 23 copies of the consensus sequences CA (2 bp) is shaded.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492157&req=5

Figure 1: Nucleotide and deduced amino acid sequence of Atlantic salmon T-bet cDNA. Uppercase denotes the UTR’s and lowercase denotes the coding regions. The T-box DNA binding domain is underlined. Start and stop codons are marked with bold letters. The asterisk indicates the stop codon. The putative polyadenylation signal is bold and underlined. A region of repeat with 23 copies of the consensus sequences CA (2 bp) is shaded.
Mentions: To obtain full-length cDNA of AsT-bet, 5′ and 3′ RACE were performed using spleen derived cDNA as template. The Atlantic salmon T-bet cDNA sequence (GenBank accession no. GU979861) consisted of 3502 bp in length including 339 bp 5′ untranslated region (UTR), a 1324 bp 3′-UTR and possessed an open reading frame of 1839 bp (Figure 1). In the 3′-UTR, there is a polyadenylation signal (AATAAA) 21 bp upstream of poly-A tail and in addition, one region of repeat with 23 copies of the consensus sequence CA (2 bp) was also identified in the 3′-UTR using tandem repeats finder program (34). The putative T-bet protein in Atlantic salmon was predicted to be 612 aa long, with a calculated molecular weight of 67.8 kDa and a pI of 8.02. The conserved T-box DNA binding domain consisted of 196 aa.

Bottom Line: Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet.Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels.Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø , Tromsø , Norway ; Nofima , Tromsø , Norway.

ABSTRACT
The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 bp). The putative primary structure of the polypeptide deduced from the cDNA sequence contained 612 aa, which possessed a T-box DNA binding domain. Phylogenetic study and gene synteny revealed it is as a homolog to mammalian T-bet. Quantitative PCR analysis of different tissues in healthy fish showed that salmon T-bet gene was highly expressed in spleen, followed by head kidney, and was expressed in intestine, skin, and liver at lower levels. Moreover, the time-dependent expression profile of T-bet, interferon gamma (IFNγ), interleukin-22 (IL-22), and natural killer enhancement factor in mucosal tissues during water-borne infection with live Aeromonas salmonicida, indicated the involvement of T-bet in mucosal immune response in Atlantic salmon.

No MeSH data available.


Related in: MedlinePlus