Limits...
Mucosal Alpha-Papillomavirus (HPV89) in a rare skin lesion.

Paolini F, Cota C, Amantea A, Curzio G, Venuti A - Virol. J. (2015)

Bottom Line: Furthermore rolling circle amplification, RT-PCR and in situ hybridization confirmed the presence of a transcriptionally active HPV 89.The association of low risk mucosal HPV infection with this skin lesion opens new perspectives in its clinical management.Further studies on samples from other patients are needed to confirm this association.

View Article: PubMed Central - PubMed

Affiliation: HPV-UNIT Laboratory of Virology, Regina Elena National Cancer Institute, via Chianesi 53, 00144, Rome, Italy. paolinifrancesca@hotmail.com.

ABSTRACT

Background: Apocrine acrosyringeal keratosis is a rare skin lesion showing a unique benign keratotic lesion associated with syringocystoadenoma papilliferum. It is characterized by an exophytic proliferation of the epidermis with two distinct keratinocytic structures: a) columns of hyperkeratotic mass surrounded by acanthotic epidermis and b) papillated and/or cystic invaginations typical of syringocystoadenoma papilliferum. No causative agents were reported.

Findings: The present report describes a typical case of apocrine acrosyringeal keratosis localized in the right retro-auricular area of 57-year-old man in which the presence of HPV was evaluated. PCR analysis and direct sequencing revealed the presence of HPV 89. The presence of this low risk mucosal HPV in a skin localization was never reported as well as in association with this rare tumor. Furthermore rolling circle amplification, RT-PCR and in situ hybridization confirmed the presence of a transcriptionally active HPV 89.

Conclusions: Taken together our results suggest that HPV89 plays a role in apocrine acrosyringeal keratosis with syringocystoadenoma papilliferum, in consideration of the documented biological activity of the virus. The association of low risk mucosal HPV infection with this skin lesion opens new perspectives in its clinical management. Further studies on samples from other patients are needed to confirm this association.

No MeSH data available.


Related in: MedlinePlus

HPV detection and typing. DNA from clinical sample was analysed for HPV detection by PCR with CP degenerate primers that amplify a broad spectrum of HPVs. PCR conditions were 3 mM MgCl2, 200 μM dNTPs, 0.5 μM for each primer and 2.5 U of Platinum TaqDNA polymerase (Life technologies, Milan, Italy) in a final reaction volume of 50 μl. Amplified products were sequenced in an automatic apparatus (Genechron Biogen, Rome, Italy) and sequence analyses were performed using BLAST program (http://www.ncbi.nlm.nih.gov/BLAST). W12 is a cell line containing episomal HPV16 utilized as positive control. M, molecular weight marker VIII (Roche, Milan, Italy)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4492091&req=5

Fig2: HPV detection and typing. DNA from clinical sample was analysed for HPV detection by PCR with CP degenerate primers that amplify a broad spectrum of HPVs. PCR conditions were 3 mM MgCl2, 200 μM dNTPs, 0.5 μM for each primer and 2.5 U of Platinum TaqDNA polymerase (Life technologies, Milan, Italy) in a final reaction volume of 50 μl. Amplified products were sequenced in an automatic apparatus (Genechron Biogen, Rome, Italy) and sequence analyses were performed using BLAST program (http://www.ncbi.nlm.nih.gov/BLAST). W12 is a cell line containing episomal HPV16 utilized as positive control. M, molecular weight marker VIII (Roche, Milan, Italy)

Mentions: PCR analysis demonstrated the presence of an amplified band that upon direct sequencing revealed a nucleotide sequence with 98 % homology to that of HPV 89 (Fig. 2). RCA showed a band of about 8000 bp after digestion with single cutting BamHI restriction enzyme, indicating the presence of episomal virus. This band was higher than that of episomal HPV16 extracted from W12 control cells because these two viruses are different in genome length (Fig. 1d). To confirm that RCA product was from HPV 89, RCA products were digested with another single cutting enzyme (KpnI), amplified with CP degenerate primers and subjected to direct sequencing that confirmed the presence of HPV type 89.Fig. 2


Mucosal Alpha-Papillomavirus (HPV89) in a rare skin lesion.

Paolini F, Cota C, Amantea A, Curzio G, Venuti A - Virol. J. (2015)

HPV detection and typing. DNA from clinical sample was analysed for HPV detection by PCR with CP degenerate primers that amplify a broad spectrum of HPVs. PCR conditions were 3 mM MgCl2, 200 μM dNTPs, 0.5 μM for each primer and 2.5 U of Platinum TaqDNA polymerase (Life technologies, Milan, Italy) in a final reaction volume of 50 μl. Amplified products were sequenced in an automatic apparatus (Genechron Biogen, Rome, Italy) and sequence analyses were performed using BLAST program (http://www.ncbi.nlm.nih.gov/BLAST). W12 is a cell line containing episomal HPV16 utilized as positive control. M, molecular weight marker VIII (Roche, Milan, Italy)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4492091&req=5

Fig2: HPV detection and typing. DNA from clinical sample was analysed for HPV detection by PCR with CP degenerate primers that amplify a broad spectrum of HPVs. PCR conditions were 3 mM MgCl2, 200 μM dNTPs, 0.5 μM for each primer and 2.5 U of Platinum TaqDNA polymerase (Life technologies, Milan, Italy) in a final reaction volume of 50 μl. Amplified products were sequenced in an automatic apparatus (Genechron Biogen, Rome, Italy) and sequence analyses were performed using BLAST program (http://www.ncbi.nlm.nih.gov/BLAST). W12 is a cell line containing episomal HPV16 utilized as positive control. M, molecular weight marker VIII (Roche, Milan, Italy)
Mentions: PCR analysis demonstrated the presence of an amplified band that upon direct sequencing revealed a nucleotide sequence with 98 % homology to that of HPV 89 (Fig. 2). RCA showed a band of about 8000 bp after digestion with single cutting BamHI restriction enzyme, indicating the presence of episomal virus. This band was higher than that of episomal HPV16 extracted from W12 control cells because these two viruses are different in genome length (Fig. 1d). To confirm that RCA product was from HPV 89, RCA products were digested with another single cutting enzyme (KpnI), amplified with CP degenerate primers and subjected to direct sequencing that confirmed the presence of HPV type 89.Fig. 2

Bottom Line: Furthermore rolling circle amplification, RT-PCR and in situ hybridization confirmed the presence of a transcriptionally active HPV 89.The association of low risk mucosal HPV infection with this skin lesion opens new perspectives in its clinical management.Further studies on samples from other patients are needed to confirm this association.

View Article: PubMed Central - PubMed

Affiliation: HPV-UNIT Laboratory of Virology, Regina Elena National Cancer Institute, via Chianesi 53, 00144, Rome, Italy. paolinifrancesca@hotmail.com.

ABSTRACT

Background: Apocrine acrosyringeal keratosis is a rare skin lesion showing a unique benign keratotic lesion associated with syringocystoadenoma papilliferum. It is characterized by an exophytic proliferation of the epidermis with two distinct keratinocytic structures: a) columns of hyperkeratotic mass surrounded by acanthotic epidermis and b) papillated and/or cystic invaginations typical of syringocystoadenoma papilliferum. No causative agents were reported.

Findings: The present report describes a typical case of apocrine acrosyringeal keratosis localized in the right retro-auricular area of 57-year-old man in which the presence of HPV was evaluated. PCR analysis and direct sequencing revealed the presence of HPV 89. The presence of this low risk mucosal HPV in a skin localization was never reported as well as in association with this rare tumor. Furthermore rolling circle amplification, RT-PCR and in situ hybridization confirmed the presence of a transcriptionally active HPV 89.

Conclusions: Taken together our results suggest that HPV89 plays a role in apocrine acrosyringeal keratosis with syringocystoadenoma papilliferum, in consideration of the documented biological activity of the virus. The association of low risk mucosal HPV infection with this skin lesion opens new perspectives in its clinical management. Further studies on samples from other patients are needed to confirm this association.

No MeSH data available.


Related in: MedlinePlus