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Mucosal Alpha-Papillomavirus (HPV89) in a rare skin lesion.

Paolini F, Cota C, Amantea A, Curzio G, Venuti A - Virol. J. (2015)

Bottom Line: Furthermore rolling circle amplification, RT-PCR and in situ hybridization confirmed the presence of a transcriptionally active HPV 89.The association of low risk mucosal HPV infection with this skin lesion opens new perspectives in its clinical management.Further studies on samples from other patients are needed to confirm this association.

View Article: PubMed Central - PubMed

Affiliation: HPV-UNIT Laboratory of Virology, Regina Elena National Cancer Institute, via Chianesi 53, 00144, Rome, Italy. paolinifrancesca@hotmail.com.

ABSTRACT

Background: Apocrine acrosyringeal keratosis is a rare skin lesion showing a unique benign keratotic lesion associated with syringocystoadenoma papilliferum. It is characterized by an exophytic proliferation of the epidermis with two distinct keratinocytic structures: a) columns of hyperkeratotic mass surrounded by acanthotic epidermis and b) papillated and/or cystic invaginations typical of syringocystoadenoma papilliferum. No causative agents were reported.

Findings: The present report describes a typical case of apocrine acrosyringeal keratosis localized in the right retro-auricular area of 57-year-old man in which the presence of HPV was evaluated. PCR analysis and direct sequencing revealed the presence of HPV 89. The presence of this low risk mucosal HPV in a skin localization was never reported as well as in association with this rare tumor. Furthermore rolling circle amplification, RT-PCR and in situ hybridization confirmed the presence of a transcriptionally active HPV 89.

Conclusions: Taken together our results suggest that HPV89 plays a role in apocrine acrosyringeal keratosis with syringocystoadenoma papilliferum, in consideration of the documented biological activity of the virus. The association of low risk mucosal HPV infection with this skin lesion opens new perspectives in its clinical management. Further studies on samples from other patients are needed to confirm this association.

No MeSH data available.


Related in: MedlinePlus

Histological findings and HPV detection. a Apocrine acrosyringeal keratosis associated with syringocystoadenoma papilliferum. Higher magnification of a warty-like area of apocrine acrosyringeal keratosis (b) and of a cystic area of syringocystoadenoma papilliferum (c). The arrow in the inset indicates a cell with perinuclear halo suggestive of a possible HPV infection. d RCA. Genomic DNA extracted and purified from the paraffin embedded sample was amplified with TempliPhi Amplification Kit (Amersham Biosciences, Milan, Italy) according to the manufacturer's instruction except an higher (450 mM) concentration of nucleotides. To resolve the concatemers, RCA products were digested with BamHI, (Invitrogen-Life Technologies, Monza, Italy), for 3 h at 37 °C in a total volume of 20 μl. Digestion products were resolved by 0.8 % agarose gel electrophoresis. W12 is a cell line containing episomal HPV16. M is a Xlarge DNA Ladder (GeneDirex,Rome, Italy). The arrow indicates the amplified band of about 8000 bp. e RT-PCR. Total RNA extracted and purified from the paraffin embedded sample was subjected to retro-transcription and nested PCR with degenerate primers. Amplified products were resolved in ethidium bromide stained agarose gel. W12 is a cell line expressing HPV16 mRNA. No RT is a control in which reverse transcriptase was omitted to exclude the presence of contaminating genomic DNA. M5 is a DNA Molecular Weight Marker V (Roche, Milan, Italy). The arrow indicates the amplified products. f ISH: In situ hybridization was performed with ZytoFast kit (Bioptica, Milan, Italy). Specific probes for HPV 89 were prepared by the asymmetric PCR [16] with consensus primers. The arrows indicate cells with HPV positive green/blue stained nuclei. Since consecutive sections were not available, the corresponding localization in H/E preparation cannot be displayed
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Fig1: Histological findings and HPV detection. a Apocrine acrosyringeal keratosis associated with syringocystoadenoma papilliferum. Higher magnification of a warty-like area of apocrine acrosyringeal keratosis (b) and of a cystic area of syringocystoadenoma papilliferum (c). The arrow in the inset indicates a cell with perinuclear halo suggestive of a possible HPV infection. d RCA. Genomic DNA extracted and purified from the paraffin embedded sample was amplified with TempliPhi Amplification Kit (Amersham Biosciences, Milan, Italy) according to the manufacturer's instruction except an higher (450 mM) concentration of nucleotides. To resolve the concatemers, RCA products were digested with BamHI, (Invitrogen-Life Technologies, Monza, Italy), for 3 h at 37 °C in a total volume of 20 μl. Digestion products were resolved by 0.8 % agarose gel electrophoresis. W12 is a cell line containing episomal HPV16. M is a Xlarge DNA Ladder (GeneDirex,Rome, Italy). The arrow indicates the amplified band of about 8000 bp. e RT-PCR. Total RNA extracted and purified from the paraffin embedded sample was subjected to retro-transcription and nested PCR with degenerate primers. Amplified products were resolved in ethidium bromide stained agarose gel. W12 is a cell line expressing HPV16 mRNA. No RT is a control in which reverse transcriptase was omitted to exclude the presence of contaminating genomic DNA. M5 is a DNA Molecular Weight Marker V (Roche, Milan, Italy). The arrow indicates the amplified products. f ISH: In situ hybridization was performed with ZytoFast kit (Bioptica, Milan, Italy). Specific probes for HPV 89 were prepared by the asymmetric PCR [16] with consensus primers. The arrows indicate cells with HPV positive green/blue stained nuclei. Since consecutive sections were not available, the corresponding localization in H/E preparation cannot be displayed

Mentions: This study was approved by the local Ethical Committee (Prot.n. CE/312/05). A 57-year-old man was showing a lesion in the right retro-auricular area that was diagnosed as apocrine acrosyringeal keratosis. Anything relevant to this pathology was in the clinical history of the patient and in particular no evidence of immunosuppression. The lesion was exophytic and revealed two different components: a well-developed syringocystoadenoma papilliferum (SCAP) with free floating papillae and a more verrucous proliferative area resembling a wart (Fig. 1a,b,c). This wart-like area showed acanthosis and papillomatosis of the epitelium with focal trichilemmal keratinization; in some areas the presence of hypergranulosis, columns of parakeratosis and perinuclear halo were suggestive of a possible HPV infection (Fig. 1b high magnification inset). Therefore, we decided to ascertain the presence of HPV by different methods.Fig. 1


Mucosal Alpha-Papillomavirus (HPV89) in a rare skin lesion.

Paolini F, Cota C, Amantea A, Curzio G, Venuti A - Virol. J. (2015)

Histological findings and HPV detection. a Apocrine acrosyringeal keratosis associated with syringocystoadenoma papilliferum. Higher magnification of a warty-like area of apocrine acrosyringeal keratosis (b) and of a cystic area of syringocystoadenoma papilliferum (c). The arrow in the inset indicates a cell with perinuclear halo suggestive of a possible HPV infection. d RCA. Genomic DNA extracted and purified from the paraffin embedded sample was amplified with TempliPhi Amplification Kit (Amersham Biosciences, Milan, Italy) according to the manufacturer's instruction except an higher (450 mM) concentration of nucleotides. To resolve the concatemers, RCA products were digested with BamHI, (Invitrogen-Life Technologies, Monza, Italy), for 3 h at 37 °C in a total volume of 20 μl. Digestion products were resolved by 0.8 % agarose gel electrophoresis. W12 is a cell line containing episomal HPV16. M is a Xlarge DNA Ladder (GeneDirex,Rome, Italy). The arrow indicates the amplified band of about 8000 bp. e RT-PCR. Total RNA extracted and purified from the paraffin embedded sample was subjected to retro-transcription and nested PCR with degenerate primers. Amplified products were resolved in ethidium bromide stained agarose gel. W12 is a cell line expressing HPV16 mRNA. No RT is a control in which reverse transcriptase was omitted to exclude the presence of contaminating genomic DNA. M5 is a DNA Molecular Weight Marker V (Roche, Milan, Italy). The arrow indicates the amplified products. f ISH: In situ hybridization was performed with ZytoFast kit (Bioptica, Milan, Italy). Specific probes for HPV 89 were prepared by the asymmetric PCR [16] with consensus primers. The arrows indicate cells with HPV positive green/blue stained nuclei. Since consecutive sections were not available, the corresponding localization in H/E preparation cannot be displayed
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4492091&req=5

Fig1: Histological findings and HPV detection. a Apocrine acrosyringeal keratosis associated with syringocystoadenoma papilliferum. Higher magnification of a warty-like area of apocrine acrosyringeal keratosis (b) and of a cystic area of syringocystoadenoma papilliferum (c). The arrow in the inset indicates a cell with perinuclear halo suggestive of a possible HPV infection. d RCA. Genomic DNA extracted and purified from the paraffin embedded sample was amplified with TempliPhi Amplification Kit (Amersham Biosciences, Milan, Italy) according to the manufacturer's instruction except an higher (450 mM) concentration of nucleotides. To resolve the concatemers, RCA products were digested with BamHI, (Invitrogen-Life Technologies, Monza, Italy), for 3 h at 37 °C in a total volume of 20 μl. Digestion products were resolved by 0.8 % agarose gel electrophoresis. W12 is a cell line containing episomal HPV16. M is a Xlarge DNA Ladder (GeneDirex,Rome, Italy). The arrow indicates the amplified band of about 8000 bp. e RT-PCR. Total RNA extracted and purified from the paraffin embedded sample was subjected to retro-transcription and nested PCR with degenerate primers. Amplified products were resolved in ethidium bromide stained agarose gel. W12 is a cell line expressing HPV16 mRNA. No RT is a control in which reverse transcriptase was omitted to exclude the presence of contaminating genomic DNA. M5 is a DNA Molecular Weight Marker V (Roche, Milan, Italy). The arrow indicates the amplified products. f ISH: In situ hybridization was performed with ZytoFast kit (Bioptica, Milan, Italy). Specific probes for HPV 89 were prepared by the asymmetric PCR [16] with consensus primers. The arrows indicate cells with HPV positive green/blue stained nuclei. Since consecutive sections were not available, the corresponding localization in H/E preparation cannot be displayed
Mentions: This study was approved by the local Ethical Committee (Prot.n. CE/312/05). A 57-year-old man was showing a lesion in the right retro-auricular area that was diagnosed as apocrine acrosyringeal keratosis. Anything relevant to this pathology was in the clinical history of the patient and in particular no evidence of immunosuppression. The lesion was exophytic and revealed two different components: a well-developed syringocystoadenoma papilliferum (SCAP) with free floating papillae and a more verrucous proliferative area resembling a wart (Fig. 1a,b,c). This wart-like area showed acanthosis and papillomatosis of the epitelium with focal trichilemmal keratinization; in some areas the presence of hypergranulosis, columns of parakeratosis and perinuclear halo were suggestive of a possible HPV infection (Fig. 1b high magnification inset). Therefore, we decided to ascertain the presence of HPV by different methods.Fig. 1

Bottom Line: Furthermore rolling circle amplification, RT-PCR and in situ hybridization confirmed the presence of a transcriptionally active HPV 89.The association of low risk mucosal HPV infection with this skin lesion opens new perspectives in its clinical management.Further studies on samples from other patients are needed to confirm this association.

View Article: PubMed Central - PubMed

Affiliation: HPV-UNIT Laboratory of Virology, Regina Elena National Cancer Institute, via Chianesi 53, 00144, Rome, Italy. paolinifrancesca@hotmail.com.

ABSTRACT

Background: Apocrine acrosyringeal keratosis is a rare skin lesion showing a unique benign keratotic lesion associated with syringocystoadenoma papilliferum. It is characterized by an exophytic proliferation of the epidermis with two distinct keratinocytic structures: a) columns of hyperkeratotic mass surrounded by acanthotic epidermis and b) papillated and/or cystic invaginations typical of syringocystoadenoma papilliferum. No causative agents were reported.

Findings: The present report describes a typical case of apocrine acrosyringeal keratosis localized in the right retro-auricular area of 57-year-old man in which the presence of HPV was evaluated. PCR analysis and direct sequencing revealed the presence of HPV 89. The presence of this low risk mucosal HPV in a skin localization was never reported as well as in association with this rare tumor. Furthermore rolling circle amplification, RT-PCR and in situ hybridization confirmed the presence of a transcriptionally active HPV 89.

Conclusions: Taken together our results suggest that HPV89 plays a role in apocrine acrosyringeal keratosis with syringocystoadenoma papilliferum, in consideration of the documented biological activity of the virus. The association of low risk mucosal HPV infection with this skin lesion opens new perspectives in its clinical management. Further studies on samples from other patients are needed to confirm this association.

No MeSH data available.


Related in: MedlinePlus