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Sex Differences in the Reinstatement of Methamphetamine Seeking after Forced Abstinence in Sprague-Dawley Rats.

Ruda-Kucerova J, Amchova P, Babinska Z, Dusek L, Micale V, Sulcova A - Front Psychiatry (2015)

Bottom Line: Females were found to self-administer significantly lower dose of METH.Females displayed approximately 300% of active nose-pokes compared to 50% in males.This effect was detected in all females, independently of current phase of their estrous cycle.

View Article: PubMed Central - PubMed

Affiliation: Experimental and Applied Neuropsychopharmacology Group, Central European Institute of Technology (CEITEC), Masaryk University , Brno , Czech Republic ; Department of Pharmacology, Faculty of Medicine, Masaryk University , Brno , Czech Republic.

ABSTRACT
Preventing relapse to drug abuse is one of the struggles faced by clinicians in order to treat patients with substance use disorders (DSM-5). There is a large body of clinical evidence suggesting differential characteristics of the disorder in men and women, which is in line with preclinical findings as well. The aim of this study was to assess differences in relapse-like behavior in methamphetamine (METH) seeking after a period of forced abstinence, which simulates the real clinical situation very well. Findings from such study might add new insights in gender differences in relapse mechanisms to previous studies, which employ a classical drug or cue-induced reinstatement procedure following the extinction training. Adult male and female Sprague-Dawley rats were used in IV self-administration procedure conducted in operant boxes using nose-poke operandi (Coulborn Instruments, USA). Active nose-poke resulted in activation of the infusion pump to deliver one intravenous infusion of METH (0.08 mg/kg). After baseline drug intake was established (maintenance phase), a period of forced abstinence was initiated and rats were kept singly in their home cages for 14 days. Finally, one reinstatement session in operant boxes was conducted. Females were found to self-administer significantly lower dose of METH. The relapse rate was assessed as a number of active nose-pokes during the reinstatement session, expressed as a percentage of active nose-poking during the maintenance phase. Females displayed approximately 300% of active nose-pokes compared to 50% in males. This indicates higher vulnerability to relapse of METH seeking behavior in female rats. This effect was detected in all females, independently of current phase of their estrous cycle. Therefore, this paradigm using operant drug self-administration and reinstatement of drug-seeking after forced abstinence model can be used for preclinical screening for potential new anti-relapse medications specific for women.

No MeSH data available.


Related in: MedlinePlus

Male and female rats have the same basal behavioral profile. Total distance traveled (in centimeters), number of rearing episodes (vertical activity), and % of preference of the central part of the arena did not reveal any gender difference. Data are shown as means (±SEM), t-test, n.s.
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Figure 1: Male and female rats have the same basal behavioral profile. Total distance traveled (in centimeters), number of rearing episodes (vertical activity), and % of preference of the central part of the arena did not reveal any gender difference. Data are shown as means (±SEM), t-test, n.s.

Mentions: Before starting the IV self-administration protocol, basal locomotor and exploratory activity was assessed in both males and females to exclude the possibility that these characteristics would lead to different drug taking behavior. Horizontal and vertical locomotor activity was measured and a proportion of each in the inner zone of the arena was calculated in order to evaluate differences in the status of anxiety in male and female rats. Figure 1 illustrates the results on total distance traveled, vertical activity (number of rearing episodes), and inner part of arena preference. There were no basal behavioral differences between the sexes, which could contribute to dissimilar behavior in the operant cage. As expected, both sexes avoided the central part of the arena, which represents normal rodent behavior and neither one shows highly anxiogenic behavior or locomotor hyper- or hypo-activity.


Sex Differences in the Reinstatement of Methamphetamine Seeking after Forced Abstinence in Sprague-Dawley Rats.

Ruda-Kucerova J, Amchova P, Babinska Z, Dusek L, Micale V, Sulcova A - Front Psychiatry (2015)

Male and female rats have the same basal behavioral profile. Total distance traveled (in centimeters), number of rearing episodes (vertical activity), and % of preference of the central part of the arena did not reveal any gender difference. Data are shown as means (±SEM), t-test, n.s.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492081&req=5

Figure 1: Male and female rats have the same basal behavioral profile. Total distance traveled (in centimeters), number of rearing episodes (vertical activity), and % of preference of the central part of the arena did not reveal any gender difference. Data are shown as means (±SEM), t-test, n.s.
Mentions: Before starting the IV self-administration protocol, basal locomotor and exploratory activity was assessed in both males and females to exclude the possibility that these characteristics would lead to different drug taking behavior. Horizontal and vertical locomotor activity was measured and a proportion of each in the inner zone of the arena was calculated in order to evaluate differences in the status of anxiety in male and female rats. Figure 1 illustrates the results on total distance traveled, vertical activity (number of rearing episodes), and inner part of arena preference. There were no basal behavioral differences between the sexes, which could contribute to dissimilar behavior in the operant cage. As expected, both sexes avoided the central part of the arena, which represents normal rodent behavior and neither one shows highly anxiogenic behavior or locomotor hyper- or hypo-activity.

Bottom Line: Females were found to self-administer significantly lower dose of METH.Females displayed approximately 300% of active nose-pokes compared to 50% in males.This effect was detected in all females, independently of current phase of their estrous cycle.

View Article: PubMed Central - PubMed

Affiliation: Experimental and Applied Neuropsychopharmacology Group, Central European Institute of Technology (CEITEC), Masaryk University , Brno , Czech Republic ; Department of Pharmacology, Faculty of Medicine, Masaryk University , Brno , Czech Republic.

ABSTRACT
Preventing relapse to drug abuse is one of the struggles faced by clinicians in order to treat patients with substance use disorders (DSM-5). There is a large body of clinical evidence suggesting differential characteristics of the disorder in men and women, which is in line with preclinical findings as well. The aim of this study was to assess differences in relapse-like behavior in methamphetamine (METH) seeking after a period of forced abstinence, which simulates the real clinical situation very well. Findings from such study might add new insights in gender differences in relapse mechanisms to previous studies, which employ a classical drug or cue-induced reinstatement procedure following the extinction training. Adult male and female Sprague-Dawley rats were used in IV self-administration procedure conducted in operant boxes using nose-poke operandi (Coulborn Instruments, USA). Active nose-poke resulted in activation of the infusion pump to deliver one intravenous infusion of METH (0.08 mg/kg). After baseline drug intake was established (maintenance phase), a period of forced abstinence was initiated and rats were kept singly in their home cages for 14 days. Finally, one reinstatement session in operant boxes was conducted. Females were found to self-administer significantly lower dose of METH. The relapse rate was assessed as a number of active nose-pokes during the reinstatement session, expressed as a percentage of active nose-poking during the maintenance phase. Females displayed approximately 300% of active nose-pokes compared to 50% in males. This indicates higher vulnerability to relapse of METH seeking behavior in female rats. This effect was detected in all females, independently of current phase of their estrous cycle. Therefore, this paradigm using operant drug self-administration and reinstatement of drug-seeking after forced abstinence model can be used for preclinical screening for potential new anti-relapse medications specific for women.

No MeSH data available.


Related in: MedlinePlus