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Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men.

Rao M, Zhao XL, Yang J, Hu SF, Lei H, Xia W, Zhu CH - Asian J. Androl. (2015 Jul-Aug)

Bottom Line: At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively).The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031).We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process.

View Article: PubMed Central - PubMed

Affiliation: Family Planning Research Institute; Reproductive Medicine Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT
In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group) received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively). The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031). We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

No MeSH data available.


Related in: MedlinePlus

Mean (±s.e.m.) seminal plasma SOD (a), CAT (b) and MDA (c) content before and after hyperthermia in the 2 groups of subjects. *P < 0.05 when compared with baseline in Group 1; **P < 0.01 when compared with baseline in Group 1; #P < 0.05 when compared with baseline in Group 2; ##P < 0.01 when compared with baseline in Group 2. SOD: superoxide dismutase; CAT: catalase; MDA: malondialdehyde.
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Figure 6: Mean (±s.e.m.) seminal plasma SOD (a), CAT (b) and MDA (c) content before and after hyperthermia in the 2 groups of subjects. *P < 0.05 when compared with baseline in Group 1; **P < 0.01 when compared with baseline in Group 1; #P < 0.05 when compared with baseline in Group 2; ##P < 0.01 when compared with baseline in Group 2. SOD: superoxide dismutase; CAT: catalase; MDA: malondialdehyde.

Mentions: Seminal plasma SOD showed no obvious variation at each time point compared with the baseline levels for both groups (P > 0.05). CAT also showed no obvious change except that the value tested at week 4 significantly decreased compared with the baseline level in Group 1 (P = 0.017). MDA levels for Group 1 increased significantly from week 2 to week 10, compared with the baseline level, with the highest value reached at week 8 (P = 0.005 when the maximum was compared with baseline level). MDA levels for group 2 increased significantly at week 8 compared with the baseline level (P = 0.017) (Figure 6).


Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men.

Rao M, Zhao XL, Yang J, Hu SF, Lei H, Xia W, Zhu CH - Asian J. Androl. (2015 Jul-Aug)

Mean (±s.e.m.) seminal plasma SOD (a), CAT (b) and MDA (c) content before and after hyperthermia in the 2 groups of subjects. *P < 0.05 when compared with baseline in Group 1; **P < 0.01 when compared with baseline in Group 1; #P < 0.05 when compared with baseline in Group 2; ##P < 0.01 when compared with baseline in Group 2. SOD: superoxide dismutase; CAT: catalase; MDA: malondialdehyde.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492061&req=5

Figure 6: Mean (±s.e.m.) seminal plasma SOD (a), CAT (b) and MDA (c) content before and after hyperthermia in the 2 groups of subjects. *P < 0.05 when compared with baseline in Group 1; **P < 0.01 when compared with baseline in Group 1; #P < 0.05 when compared with baseline in Group 2; ##P < 0.01 when compared with baseline in Group 2. SOD: superoxide dismutase; CAT: catalase; MDA: malondialdehyde.
Mentions: Seminal plasma SOD showed no obvious variation at each time point compared with the baseline levels for both groups (P > 0.05). CAT also showed no obvious change except that the value tested at week 4 significantly decreased compared with the baseline level in Group 1 (P = 0.017). MDA levels for Group 1 increased significantly from week 2 to week 10, compared with the baseline level, with the highest value reached at week 8 (P = 0.005 when the maximum was compared with baseline level). MDA levels for group 2 increased significantly at week 8 compared with the baseline level (P = 0.017) (Figure 6).

Bottom Line: At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively).The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031).We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process.

View Article: PubMed Central - PubMed

Affiliation: Family Planning Research Institute; Reproductive Medicine Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT
In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group) received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively). The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031). We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

No MeSH data available.


Related in: MedlinePlus