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Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men.

Rao M, Zhao XL, Yang J, Hu SF, Lei H, Xia W, Zhu CH - Asian J. Androl. (2015 Jul-Aug)

Bottom Line: At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively).The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031).This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

View Article: PubMed Central - PubMed

Affiliation: Family Planning Research Institute; Reproductive Medicine Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT
In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group) received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively). The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031). We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

No MeSH data available.


Related in: MedlinePlus

Mean (±s.e.m.) sperm hypo-osmotic swelling rate (a), sperm total acrosin activity (b) before and after treatment in the 2 groups of subjects. There were 3, 3 and 1 values missing at week 6, 8 and 10 respectively in Group 1 due to low value of total sperm count; there were 1, 5, 8, 1 and 1 values missing at week 4, 6, 8, 10 and 12 respectively in Group 2 due to low value of total sperm count. *P < 0.05 when compared with baseline in Group 1; **P < 0.01 when compared with baseline in Group 1; #P < 0.05 when compared with baseline in Group 2; ##P < 0.01 when compared with baseline in Group 2.
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Figure 3: Mean (±s.e.m.) sperm hypo-osmotic swelling rate (a), sperm total acrosin activity (b) before and after treatment in the 2 groups of subjects. There were 3, 3 and 1 values missing at week 6, 8 and 10 respectively in Group 1 due to low value of total sperm count; there were 1, 5, 8, 1 and 1 values missing at week 4, 6, 8, 10 and 12 respectively in Group 2 due to low value of total sperm count. *P < 0.05 when compared with baseline in Group 1; **P < 0.01 when compared with baseline in Group 1; #P < 0.05 when compared with baseline in Group 2; ##P < 0.01 when compared with baseline in Group 2.

Mentions: The mean value of the tail swelling rate for Group 1 decreased significantly from week 4 to 8 compared with the baseline level (P = 0.007 when the minimum was compared with baseline level), recovering at week 10. The value for group 2 decreased significantly from week 6 to 10, compared with the baseline level (P = 0.008 when the minimum was compared with the baseline level), recovering at week 12. The lowest values for both groups appeared at week 6 (Figure 3a).


Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men.

Rao M, Zhao XL, Yang J, Hu SF, Lei H, Xia W, Zhu CH - Asian J. Androl. (2015 Jul-Aug)

Mean (±s.e.m.) sperm hypo-osmotic swelling rate (a), sperm total acrosin activity (b) before and after treatment in the 2 groups of subjects. There were 3, 3 and 1 values missing at week 6, 8 and 10 respectively in Group 1 due to low value of total sperm count; there were 1, 5, 8, 1 and 1 values missing at week 4, 6, 8, 10 and 12 respectively in Group 2 due to low value of total sperm count. *P < 0.05 when compared with baseline in Group 1; **P < 0.01 when compared with baseline in Group 1; #P < 0.05 when compared with baseline in Group 2; ##P < 0.01 when compared with baseline in Group 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492061&req=5

Figure 3: Mean (±s.e.m.) sperm hypo-osmotic swelling rate (a), sperm total acrosin activity (b) before and after treatment in the 2 groups of subjects. There were 3, 3 and 1 values missing at week 6, 8 and 10 respectively in Group 1 due to low value of total sperm count; there were 1, 5, 8, 1 and 1 values missing at week 4, 6, 8, 10 and 12 respectively in Group 2 due to low value of total sperm count. *P < 0.05 when compared with baseline in Group 1; **P < 0.01 when compared with baseline in Group 1; #P < 0.05 when compared with baseline in Group 2; ##P < 0.01 when compared with baseline in Group 2.
Mentions: The mean value of the tail swelling rate for Group 1 decreased significantly from week 4 to 8 compared with the baseline level (P = 0.007 when the minimum was compared with baseline level), recovering at week 10. The value for group 2 decreased significantly from week 6 to 10, compared with the baseline level (P = 0.008 when the minimum was compared with the baseline level), recovering at week 12. The lowest values for both groups appeared at week 6 (Figure 3a).

Bottom Line: At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively).The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031).This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

View Article: PubMed Central - PubMed

Affiliation: Family Planning Research Institute; Reproductive Medicine Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT
In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group) received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively). The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031). We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

No MeSH data available.


Related in: MedlinePlus