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Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men.

Rao M, Zhao XL, Yang J, Hu SF, Lei H, Xia W, Zhu CH - Asian J. Androl. (2015 Jul-Aug)

Bottom Line: At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively).The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031).This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

View Article: PubMed Central - PubMed

Affiliation: Family Planning Research Institute; Reproductive Medicine Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT
In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group) received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively). The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031). We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

No MeSH data available.


Related in: MedlinePlus

Number of subjects in each treatment group in which the sperm concentration was suppressed to severe oligozoospermia (less than 5 million ml−1; black bars) and oligozoospermia (less than 15 million ml−1; black bars plus dark hatched bars).
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Figure 2: Number of subjects in each treatment group in which the sperm concentration was suppressed to severe oligozoospermia (less than 5 million ml−1; black bars) and oligozoospermia (less than 15 million ml−1; black bars plus dark hatched bars).

Mentions: The sperm concentration and total sperm count of both groups showed reversible decreases. The minimum levels of sperm concentration observed at week eight after treatment were significantly different from baseline levels (P = 0.005 for Group 1 and P = 0.008 for Group 2). The minimum levels of total sperm count observed at week 6 or 8 after treatment were also significantly different from baseline levels (P = 0.009 for Group 1 and P = 0.005 for Group 2). The sperm concentration of Group 2 (week 8: 15.5% of baseline value) decreased more drastically than that of Group 1 (week 8: 28.8% of baseline value), and the Group 2 concentrations recovered more slowly (Figures 1 and 2). The magnitude of sperm concentration reduction for Group 2 was greater than for Group 1 (P = 0.031). Sperm progressive motility decreased in both groups and followed a similar pattern to that of sperm concentration, with the lowest value recorded at 6 weeks after hyperthermia treatment, the minimum in both groups were significantly different from baseline levels (P = 0.009 for Group 1 and P = 0.021 for Group 2) (Figure 1). Furthermore, 7 and 4 subjects in Group 1 reached oligozoospermia (less than 15 million ml−1) and severe oligozoospermia (less than 5 million ml−1), respectively; 9 and 4 subjects in Group 2 reached oligozoospermia and severe oligozoospermia respectively, no significant difference existed (Figure 2).


Effect of transient scrotal hyperthermia on sperm parameters, seminal plasma biochemical markers, and oxidative stress in men.

Rao M, Zhao XL, Yang J, Hu SF, Lei H, Xia W, Zhu CH - Asian J. Androl. (2015 Jul-Aug)

Number of subjects in each treatment group in which the sperm concentration was suppressed to severe oligozoospermia (less than 5 million ml−1; black bars) and oligozoospermia (less than 15 million ml−1; black bars plus dark hatched bars).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492061&req=5

Figure 2: Number of subjects in each treatment group in which the sperm concentration was suppressed to severe oligozoospermia (less than 5 million ml−1; black bars) and oligozoospermia (less than 15 million ml−1; black bars plus dark hatched bars).
Mentions: The sperm concentration and total sperm count of both groups showed reversible decreases. The minimum levels of sperm concentration observed at week eight after treatment were significantly different from baseline levels (P = 0.005 for Group 1 and P = 0.008 for Group 2). The minimum levels of total sperm count observed at week 6 or 8 after treatment were also significantly different from baseline levels (P = 0.009 for Group 1 and P = 0.005 for Group 2). The sperm concentration of Group 2 (week 8: 15.5% of baseline value) decreased more drastically than that of Group 1 (week 8: 28.8% of baseline value), and the Group 2 concentrations recovered more slowly (Figures 1 and 2). The magnitude of sperm concentration reduction for Group 2 was greater than for Group 1 (P = 0.031). Sperm progressive motility decreased in both groups and followed a similar pattern to that of sperm concentration, with the lowest value recorded at 6 weeks after hyperthermia treatment, the minimum in both groups were significantly different from baseline levels (P = 0.009 for Group 1 and P = 0.021 for Group 2) (Figure 1). Furthermore, 7 and 4 subjects in Group 1 reached oligozoospermia (less than 15 million ml−1) and severe oligozoospermia (less than 5 million ml−1), respectively; 9 and 4 subjects in Group 2 reached oligozoospermia and severe oligozoospermia respectively, no significant difference existed (Figure 2).

Bottom Line: At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively).The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031).This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

View Article: PubMed Central - PubMed

Affiliation: Family Planning Research Institute; Reproductive Medicine Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

ABSTRACT
In this experimental prospective study, we aimed to analyze the effect of transient scrotal hyperthermia on the male reproductive organs, from the perspective of sperm parameters, semen plasma biochemical markers, and oxidative stress, to evaluate whether different frequencies of heat exposure cause different degrees of damage to spermatogenesis. Two groups of volunteers (10 per group) received testicular warming in a 43°C water bath 10 times, for 30 min each time: group 1: 10 consecutive days; group 2: once every 3 days. Sperm parameters, epididymis and accessory sex gland function, semen plasma oxidative stress and serum sex hormones were tested before treatment and in the 16-week recovery period after treatment. At last, we found an obvious reversible decrease in sperm concentration (P = 0.005 for Group 1 and P= 0.008 for Group 2 when the minimums were compared with baseline levels, the same below), motility (P = 0.009 and 0.021, respectively), the hypoosmotic swelling test score (P = 0.007 and 0.008, respectively), total acrosin activity (P = 0.018 and 0.009, respectively), and an increase in the seminal plasma malondialdehyde concentration (P = 0.005 and 0.017, respectively). The decrease of sperm concentration was greater for Group 2 than for Group 1 (P = 0.031). We concluded that transient scrotal hyperthermia seriously, but reversibly, negatively affected the spermatogenesis, oxidative stress may be involved in this process. In addition, intermittent heat exposure more seriously suppresses the spermatogenesis compared to consecutive heat exposure. This may be indicative for clinical infertility etiology analysis and the design of contraceptive methods based on heat stress.

No MeSH data available.


Related in: MedlinePlus