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The future of computer-aided sperm analysis.

Mortimer ST, van der Horst G, Mortimer D - Asian J. Androl. (2015 Jul-Aug)

Bottom Line: CASA has also been used with great success for measuring semen characteristics such as sperm concentration and proportions of progressive motility in many animal species, including wide application in domesticated animal production laboratories and reproductive toxicology.However, attempts to use CASA for human clinical semen analysis have largely met with poor success due to the inherent difficulties presented by many human semen samples caused by sperm clumping and heavy background debris that, until now, have precluded accurate digital image analysis.Specific requirements for validating CASA technology as a semi-automated system for human semen analysis are also provided, with particular reference to the accuracy and uncertainty of measurement expected of a robust medical laboratory test for implementation in clinical laboratories operating according to modern accreditation standards.

View Article: PubMed Central - PubMed

Affiliation: Oozoa Biomedical, West Vancouver, BC, Canada.

ABSTRACT
Computer-aided sperm analysis (CASA) technology was developed in the late 1980s for analyzing sperm movement characteristics or kinematics and has been highly successful in enabling this field of research. CASA has also been used with great success for measuring semen characteristics such as sperm concentration and proportions of progressive motility in many animal species, including wide application in domesticated animal production laboratories and reproductive toxicology. However, attempts to use CASA for human clinical semen analysis have largely met with poor success due to the inherent difficulties presented by many human semen samples caused by sperm clumping and heavy background debris that, until now, have precluded accurate digital image analysis. The authors review the improved capabilities of two modern CASA platforms (Hamilton Thorne CASA-II and Microptic SCA6) and consider their current and future applications with particular reference to directing our focus towards using this technology to assess functional rather than simple descriptive characteristics of spermatozoa. Specific requirements for validating CASA technology as a semi-automated system for human semen analysis are also provided, with particular reference to the accuracy and uncertainty of measurement expected of a robust medical laboratory test for implementation in clinical laboratories operating according to modern accreditation standards.

No MeSH data available.


Related in: MedlinePlus

The upper panel shows the reconstructed sperm tracks in one analysis field of the IVOS-II system (1 s @ 60 Hz), purple tracks are ones that have passed the Boolean sort argument for being hyperactivated. The lower panel shows the zoomed image of one of these tracks including the spermatozoon at the start of the track; note the high curvature wave, characteristic of a hyperactivated spermatozoon, present in the proximal region of the tail. The cell's kinematic values are shown at the bottom of the figure, including the fractal dimension, D, clearly confirming hyperactivation.60
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Figure 1: The upper panel shows the reconstructed sperm tracks in one analysis field of the IVOS-II system (1 s @ 60 Hz), purple tracks are ones that have passed the Boolean sort argument for being hyperactivated. The lower panel shows the zoomed image of one of these tracks including the spermatozoon at the start of the track; note the high curvature wave, characteristic of a hyperactivated spermatozoon, present in the proximal region of the tail. The cell's kinematic values are shown at the bottom of the figure, including the fractal dimension, D, clearly confirming hyperactivation.60

Mentions: As before, in the Results View mode individual tracks can be selected and zoomed to show the track details and the derived kinematic values, but now the individual frame images of the spermatozoon, including the proximal part of its tail, are also superimposed on the reconstructed track (Figure 1). This is not just an excellent analytical tool but also a powerful teaching tool, allowing users to understand how the beating of the sperm tail moves the sperm head and creates the track that is the basis for the kinematic analysis.


The future of computer-aided sperm analysis.

Mortimer ST, van der Horst G, Mortimer D - Asian J. Androl. (2015 Jul-Aug)

The upper panel shows the reconstructed sperm tracks in one analysis field of the IVOS-II system (1 s @ 60 Hz), purple tracks are ones that have passed the Boolean sort argument for being hyperactivated. The lower panel shows the zoomed image of one of these tracks including the spermatozoon at the start of the track; note the high curvature wave, characteristic of a hyperactivated spermatozoon, present in the proximal region of the tail. The cell's kinematic values are shown at the bottom of the figure, including the fractal dimension, D, clearly confirming hyperactivation.60
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492043&req=5

Figure 1: The upper panel shows the reconstructed sperm tracks in one analysis field of the IVOS-II system (1 s @ 60 Hz), purple tracks are ones that have passed the Boolean sort argument for being hyperactivated. The lower panel shows the zoomed image of one of these tracks including the spermatozoon at the start of the track; note the high curvature wave, characteristic of a hyperactivated spermatozoon, present in the proximal region of the tail. The cell's kinematic values are shown at the bottom of the figure, including the fractal dimension, D, clearly confirming hyperactivation.60
Mentions: As before, in the Results View mode individual tracks can be selected and zoomed to show the track details and the derived kinematic values, but now the individual frame images of the spermatozoon, including the proximal part of its tail, are also superimposed on the reconstructed track (Figure 1). This is not just an excellent analytical tool but also a powerful teaching tool, allowing users to understand how the beating of the sperm tail moves the sperm head and creates the track that is the basis for the kinematic analysis.

Bottom Line: CASA has also been used with great success for measuring semen characteristics such as sperm concentration and proportions of progressive motility in many animal species, including wide application in domesticated animal production laboratories and reproductive toxicology.However, attempts to use CASA for human clinical semen analysis have largely met with poor success due to the inherent difficulties presented by many human semen samples caused by sperm clumping and heavy background debris that, until now, have precluded accurate digital image analysis.Specific requirements for validating CASA technology as a semi-automated system for human semen analysis are also provided, with particular reference to the accuracy and uncertainty of measurement expected of a robust medical laboratory test for implementation in clinical laboratories operating according to modern accreditation standards.

View Article: PubMed Central - PubMed

Affiliation: Oozoa Biomedical, West Vancouver, BC, Canada.

ABSTRACT
Computer-aided sperm analysis (CASA) technology was developed in the late 1980s for analyzing sperm movement characteristics or kinematics and has been highly successful in enabling this field of research. CASA has also been used with great success for measuring semen characteristics such as sperm concentration and proportions of progressive motility in many animal species, including wide application in domesticated animal production laboratories and reproductive toxicology. However, attempts to use CASA for human clinical semen analysis have largely met with poor success due to the inherent difficulties presented by many human semen samples caused by sperm clumping and heavy background debris that, until now, have precluded accurate digital image analysis. The authors review the improved capabilities of two modern CASA platforms (Hamilton Thorne CASA-II and Microptic SCA6) and consider their current and future applications with particular reference to directing our focus towards using this technology to assess functional rather than simple descriptive characteristics of spermatozoa. Specific requirements for validating CASA technology as a semi-automated system for human semen analysis are also provided, with particular reference to the accuracy and uncertainty of measurement expected of a robust medical laboratory test for implementation in clinical laboratories operating according to modern accreditation standards.

No MeSH data available.


Related in: MedlinePlus