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Use of Curcuma longa L. extract to stain various tissue samples for histological studies.

Kumar S, Singh NN, Singh A, Singh N, Sinha RK - Ayu (2014 Oct-Dec)

Bottom Line: These dried turmeric rhizomes were milled to form fine powder, which was then processed to form dye for staining tissue structures.It revealed that turmeric can be used as a counterstain after hematoxylin, its staining ability was also good and comparable to that of eosin dye with a special affinity for collagen and muscle fibers.Turmeric dye can be used as a histological stain, which stains similar to eosin dye and its specific affinity for collagen and muscle fibers authenticates its role in the treatment of collagen and muscle disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Microbiology, Kothiwal Dental College and Research Centre, Moradabad, India.

ABSTRACT

Background: Curcuma longa L. is a perennial herb and a member of the Zingiberaceae (ginger) family, which is used extensively in foods as well as in Ayurvedic and Chinese systems of medicine. Current researches have focused on its antioxidant, hepatoprotective, anti-inflammatory, anticarcinogenic and antimicrobial properties. Until now, very few studies suggested its role as a histological stain.

Aim: To ascertain its efficacy to be used as a counterstain after hematoxylin, to compare it's staining ability with that of routinely used eosin dye and also to ascertain its role in various collagen diseases.

Materials and methods: Turmeric rhizomes were cut into small pieces and were dried. These dried turmeric rhizomes were milled to form fine powder, which was then processed to form dye for staining tissue structures.

Results: It revealed that turmeric can be used as a counterstain after hematoxylin, its staining ability was also good and comparable to that of eosin dye with a special affinity for collagen and muscle fibers.

Conclusion: Turmeric dye can be used as a histological stain, which stains similar to eosin dye and its specific affinity for collagen and muscle fibers authenticates its role in the treatment of collagen and muscle disorders.

No MeSH data available.


Related in: MedlinePlus

Photomicrographs of muscles showing comparison of staining ability of eosin and turmeric at ×40
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Figure 5: Photomicrographs of muscles showing comparison of staining ability of eosin and turmeric at ×40

Mentions: Ten independent oral pathologists compared the staining efficacy of turmeric with that of eosin for various tissue structures such as epithelium [Figure 3], collagen [Figure 4], muscles [Figure 5], blood vessels, nerves [Figure 6], bone [Figure 7], adipose tissue [Figure 8], and keratin [Figure 3]. For this three grading criteria were assigned:


Use of Curcuma longa L. extract to stain various tissue samples for histological studies.

Kumar S, Singh NN, Singh A, Singh N, Sinha RK - Ayu (2014 Oct-Dec)

Photomicrographs of muscles showing comparison of staining ability of eosin and turmeric at ×40
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492033&req=5

Figure 5: Photomicrographs of muscles showing comparison of staining ability of eosin and turmeric at ×40
Mentions: Ten independent oral pathologists compared the staining efficacy of turmeric with that of eosin for various tissue structures such as epithelium [Figure 3], collagen [Figure 4], muscles [Figure 5], blood vessels, nerves [Figure 6], bone [Figure 7], adipose tissue [Figure 8], and keratin [Figure 3]. For this three grading criteria were assigned:

Bottom Line: These dried turmeric rhizomes were milled to form fine powder, which was then processed to form dye for staining tissue structures.It revealed that turmeric can be used as a counterstain after hematoxylin, its staining ability was also good and comparable to that of eosin dye with a special affinity for collagen and muscle fibers.Turmeric dye can be used as a histological stain, which stains similar to eosin dye and its specific affinity for collagen and muscle fibers authenticates its role in the treatment of collagen and muscle disorders.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology and Microbiology, Kothiwal Dental College and Research Centre, Moradabad, India.

ABSTRACT

Background: Curcuma longa L. is a perennial herb and a member of the Zingiberaceae (ginger) family, which is used extensively in foods as well as in Ayurvedic and Chinese systems of medicine. Current researches have focused on its antioxidant, hepatoprotective, anti-inflammatory, anticarcinogenic and antimicrobial properties. Until now, very few studies suggested its role as a histological stain.

Aim: To ascertain its efficacy to be used as a counterstain after hematoxylin, to compare it's staining ability with that of routinely used eosin dye and also to ascertain its role in various collagen diseases.

Materials and methods: Turmeric rhizomes were cut into small pieces and were dried. These dried turmeric rhizomes were milled to form fine powder, which was then processed to form dye for staining tissue structures.

Results: It revealed that turmeric can be used as a counterstain after hematoxylin, its staining ability was also good and comparable to that of eosin dye with a special affinity for collagen and muscle fibers.

Conclusion: Turmeric dye can be used as a histological stain, which stains similar to eosin dye and its specific affinity for collagen and muscle fibers authenticates its role in the treatment of collagen and muscle disorders.

No MeSH data available.


Related in: MedlinePlus