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Dolichos biflorus Linn. ameliorates diabetic complications in streptozotocin induced diabetic rats.

Saxena Y, Purwar B, Meena H, Sarthi P - Ayu (2014 Oct-Dec)

Bottom Line: Results indicated improvement in serum creatinine levels and reduced glomerular sclerosing and Bowman's space with interstitial alterations and significantly reduced renal hypertrophy in diabetic rat son Dolichos diabetic rats (P < 0.001).Retinal layers showed inconsistent improvement in the width of the neuronal layers and decreased vacuolization of plexiform layers and retinal vessel density.D. biflorus at doses of 300 mg/kg/day for 30 days resulted in gradual but significant decreased diabetic nephropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Himalayan Institute of Medical Sciences, SRH University, Dehradun, Uttarakhand, India.

ABSTRACT

Background: Horsegram (Dolichos biflorus Linn.) is a known antilithiatic, hypolipedemic and has free radical scavenging activity and increased production of reactive oxygen species play a role in pathophysiological mechanisms that trigger diabetic complications.

Aim: To see the effect of daily oral feeding of D.biflorous on nephropathy and retinopathy in streptozotocin (STZ) induced-diabetic rats.

Materials and methods: A total of 24 healthy rats were randomly grouped into controls, diabetic and diabetic on Dolichos. Diabetes was induced by a single dose of STZ (55 mg/kg) and animals were given prepared food and water ad libitum. Dolichos was orally given at 300 mg/kg/day to rats in diabetic on Dolichos group for next 30 days. Fasting blood glucose levels was monitored at beginning and at the end of the experiment while assessment of serum creatinine levels and histopathological study of kidney and retina was carried only at the end of the experiment. Statistical differences between groups were analyzed using analysis of variance followed by, Bonferroni test as posthoc test.

Results: Results indicated improvement in serum creatinine levels and reduced glomerular sclerosing and Bowman's space with interstitial alterations and significantly reduced renal hypertrophy in diabetic rat son Dolichos diabetic rats (P < 0.001). Retinal layers showed inconsistent improvement in the width of the neuronal layers and decreased vacuolization of plexiform layers and retinal vessel density.

Conclusion: D. biflorus at doses of 300 mg/kg/day for 30 days resulted in gradual but significant decreased diabetic nephropathy.

No MeSH data available.


Related in: MedlinePlus

(a) Normal rat: All layer of retina are distinct and regularly arranged. (b) Diabetic rat ×40: Giant retinal vessels occupying the ganglion layer and inner plexiform layer. Lumen filled with red blood cells; Lucentare as seen in inner plexiform. (c) Diabetic rats on Dolichos: ×40: Less disruption of layers including photo receptors layer; few blood vessels in ganglion cell layer; little vacuolization in plexiform layer
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Figure 2: (a) Normal rat: All layer of retina are distinct and regularly arranged. (b) Diabetic rat ×40: Giant retinal vessels occupying the ganglion layer and inner plexiform layer. Lumen filled with red blood cells; Lucentare as seen in inner plexiform. (c) Diabetic rats on Dolichos: ×40: Less disruption of layers including photo receptors layer; few blood vessels in ganglion cell layer; little vacuolization in plexiform layer

Mentions: Retinal histology shows infiltration of blood vessels in the layers of retina (ganglion layer) and disruption of the plexiform layer with vacuolization and thickening of the basement membrane [Figure 2b] when compared with the normal rats [Figure 2a]. The retinal layers of the diabetic rats on Dolichos showed lesser development of the features of diabetes following 30 days of Dolichos only in five cases; however, rest of the three diabetic rats did show early changes of diabetic retinopathy (DR).


Dolichos biflorus Linn. ameliorates diabetic complications in streptozotocin induced diabetic rats.

Saxena Y, Purwar B, Meena H, Sarthi P - Ayu (2014 Oct-Dec)

(a) Normal rat: All layer of retina are distinct and regularly arranged. (b) Diabetic rat ×40: Giant retinal vessels occupying the ganglion layer and inner plexiform layer. Lumen filled with red blood cells; Lucentare as seen in inner plexiform. (c) Diabetic rats on Dolichos: ×40: Less disruption of layers including photo receptors layer; few blood vessels in ganglion cell layer; little vacuolization in plexiform layer
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492032&req=5

Figure 2: (a) Normal rat: All layer of retina are distinct and regularly arranged. (b) Diabetic rat ×40: Giant retinal vessels occupying the ganglion layer and inner plexiform layer. Lumen filled with red blood cells; Lucentare as seen in inner plexiform. (c) Diabetic rats on Dolichos: ×40: Less disruption of layers including photo receptors layer; few blood vessels in ganglion cell layer; little vacuolization in plexiform layer
Mentions: Retinal histology shows infiltration of blood vessels in the layers of retina (ganglion layer) and disruption of the plexiform layer with vacuolization and thickening of the basement membrane [Figure 2b] when compared with the normal rats [Figure 2a]. The retinal layers of the diabetic rats on Dolichos showed lesser development of the features of diabetes following 30 days of Dolichos only in five cases; however, rest of the three diabetic rats did show early changes of diabetic retinopathy (DR).

Bottom Line: Results indicated improvement in serum creatinine levels and reduced glomerular sclerosing and Bowman's space with interstitial alterations and significantly reduced renal hypertrophy in diabetic rat son Dolichos diabetic rats (P < 0.001).Retinal layers showed inconsistent improvement in the width of the neuronal layers and decreased vacuolization of plexiform layers and retinal vessel density.D. biflorus at doses of 300 mg/kg/day for 30 days resulted in gradual but significant decreased diabetic nephropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Himalayan Institute of Medical Sciences, SRH University, Dehradun, Uttarakhand, India.

ABSTRACT

Background: Horsegram (Dolichos biflorus Linn.) is a known antilithiatic, hypolipedemic and has free radical scavenging activity and increased production of reactive oxygen species play a role in pathophysiological mechanisms that trigger diabetic complications.

Aim: To see the effect of daily oral feeding of D.biflorous on nephropathy and retinopathy in streptozotocin (STZ) induced-diabetic rats.

Materials and methods: A total of 24 healthy rats were randomly grouped into controls, diabetic and diabetic on Dolichos. Diabetes was induced by a single dose of STZ (55 mg/kg) and animals were given prepared food and water ad libitum. Dolichos was orally given at 300 mg/kg/day to rats in diabetic on Dolichos group for next 30 days. Fasting blood glucose levels was monitored at beginning and at the end of the experiment while assessment of serum creatinine levels and histopathological study of kidney and retina was carried only at the end of the experiment. Statistical differences between groups were analyzed using analysis of variance followed by, Bonferroni test as posthoc test.

Results: Results indicated improvement in serum creatinine levels and reduced glomerular sclerosing and Bowman's space with interstitial alterations and significantly reduced renal hypertrophy in diabetic rat son Dolichos diabetic rats (P < 0.001). Retinal layers showed inconsistent improvement in the width of the neuronal layers and decreased vacuolization of plexiform layers and retinal vessel density.

Conclusion: D. biflorus at doses of 300 mg/kg/day for 30 days resulted in gradual but significant decreased diabetic nephropathy.

No MeSH data available.


Related in: MedlinePlus