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Dolichos biflorus Linn. ameliorates diabetic complications in streptozotocin induced diabetic rats.

Saxena Y, Purwar B, Meena H, Sarthi P - Ayu (2014 Oct-Dec)

Bottom Line: Results indicated improvement in serum creatinine levels and reduced glomerular sclerosing and Bowman's space with interstitial alterations and significantly reduced renal hypertrophy in diabetic rat son Dolichos diabetic rats (P < 0.001).Retinal layers showed inconsistent improvement in the width of the neuronal layers and decreased vacuolization of plexiform layers and retinal vessel density.D. biflorus at doses of 300 mg/kg/day for 30 days resulted in gradual but significant decreased diabetic nephropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Himalayan Institute of Medical Sciences, SRH University, Dehradun, Uttarakhand, India.

ABSTRACT

Background: Horsegram (Dolichos biflorus Linn.) is a known antilithiatic, hypolipedemic and has free radical scavenging activity and increased production of reactive oxygen species play a role in pathophysiological mechanisms that trigger diabetic complications.

Aim: To see the effect of daily oral feeding of D.biflorous on nephropathy and retinopathy in streptozotocin (STZ) induced-diabetic rats.

Materials and methods: A total of 24 healthy rats were randomly grouped into controls, diabetic and diabetic on Dolichos. Diabetes was induced by a single dose of STZ (55 mg/kg) and animals were given prepared food and water ad libitum. Dolichos was orally given at 300 mg/kg/day to rats in diabetic on Dolichos group for next 30 days. Fasting blood glucose levels was monitored at beginning and at the end of the experiment while assessment of serum creatinine levels and histopathological study of kidney and retina was carried only at the end of the experiment. Statistical differences between groups were analyzed using analysis of variance followed by, Bonferroni test as posthoc test.

Results: Results indicated improvement in serum creatinine levels and reduced glomerular sclerosing and Bowman's space with interstitial alterations and significantly reduced renal hypertrophy in diabetic rat son Dolichos diabetic rats (P < 0.001). Retinal layers showed inconsistent improvement in the width of the neuronal layers and decreased vacuolization of plexiform layers and retinal vessel density.

Conclusion: D. biflorus at doses of 300 mg/kg/day for 30 days resulted in gradual but significant decreased diabetic nephropathy.

No MeSH data available.


Related in: MedlinePlus

Normal rat: Kidney at ×20 normal Bowman's space with normal architecture and no evident sclerosis seen: Normal tissue. (b) Diabetic rat ×40: Giant retinal vessels occupying the ganglion layer and inner plexiform layer. Lumen filled with red blood cells; Lucentare as seen in inner plexiform. (c) Diabetic rat on Dolichos: Kidney at ×20: Bowman's space are not decreased with minimal disruption of architecture; protein deposits are minimal with less tubular obliteration; minimal infilteration
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Figure 1: Normal rat: Kidney at ×20 normal Bowman's space with normal architecture and no evident sclerosis seen: Normal tissue. (b) Diabetic rat ×40: Giant retinal vessels occupying the ganglion layer and inner plexiform layer. Lumen filled with red blood cells; Lucentare as seen in inner plexiform. (c) Diabetic rat on Dolichos: Kidney at ×20: Bowman's space are not decreased with minimal disruption of architecture; protein deposits are minimal with less tubular obliteration; minimal infilteration

Mentions: For the study and comparison of pancreatic islets of Langerhans, retina and kidney, rats from all the groups were sacrificed by cervical dislocation and dissected for retrieval of pancreatic tissue, enucleation of eyeballs and resection of both the kidneys. The samples were fixed in 10% formalin, stained with H and E and photographed by microscope with × 20 and × 40 magnifications. In diabetic rats tissue of pancreatic Langerhans and the beta cells degenerated irreversibly.[8] The kidneys were observed for histological changes like glomerular thickening with decreased bowmen space, tubular vacuolization and moderate interstitial fibrosis [Figure 1a–c]. H and E sections of the retina were observed for decreased neuronal layer with thickening of basement membrane, blood vessels invasion and vacuolization of plexiform layers [Figure 2a–c].


Dolichos biflorus Linn. ameliorates diabetic complications in streptozotocin induced diabetic rats.

Saxena Y, Purwar B, Meena H, Sarthi P - Ayu (2014 Oct-Dec)

Normal rat: Kidney at ×20 normal Bowman's space with normal architecture and no evident sclerosis seen: Normal tissue. (b) Diabetic rat ×40: Giant retinal vessels occupying the ganglion layer and inner plexiform layer. Lumen filled with red blood cells; Lucentare as seen in inner plexiform. (c) Diabetic rat on Dolichos: Kidney at ×20: Bowman's space are not decreased with minimal disruption of architecture; protein deposits are minimal with less tubular obliteration; minimal infilteration
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492032&req=5

Figure 1: Normal rat: Kidney at ×20 normal Bowman's space with normal architecture and no evident sclerosis seen: Normal tissue. (b) Diabetic rat ×40: Giant retinal vessels occupying the ganglion layer and inner plexiform layer. Lumen filled with red blood cells; Lucentare as seen in inner plexiform. (c) Diabetic rat on Dolichos: Kidney at ×20: Bowman's space are not decreased with minimal disruption of architecture; protein deposits are minimal with less tubular obliteration; minimal infilteration
Mentions: For the study and comparison of pancreatic islets of Langerhans, retina and kidney, rats from all the groups were sacrificed by cervical dislocation and dissected for retrieval of pancreatic tissue, enucleation of eyeballs and resection of both the kidneys. The samples were fixed in 10% formalin, stained with H and E and photographed by microscope with × 20 and × 40 magnifications. In diabetic rats tissue of pancreatic Langerhans and the beta cells degenerated irreversibly.[8] The kidneys were observed for histological changes like glomerular thickening with decreased bowmen space, tubular vacuolization and moderate interstitial fibrosis [Figure 1a–c]. H and E sections of the retina were observed for decreased neuronal layer with thickening of basement membrane, blood vessels invasion and vacuolization of plexiform layers [Figure 2a–c].

Bottom Line: Results indicated improvement in serum creatinine levels and reduced glomerular sclerosing and Bowman's space with interstitial alterations and significantly reduced renal hypertrophy in diabetic rat son Dolichos diabetic rats (P < 0.001).Retinal layers showed inconsistent improvement in the width of the neuronal layers and decreased vacuolization of plexiform layers and retinal vessel density.D. biflorus at doses of 300 mg/kg/day for 30 days resulted in gradual but significant decreased diabetic nephropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Himalayan Institute of Medical Sciences, SRH University, Dehradun, Uttarakhand, India.

ABSTRACT

Background: Horsegram (Dolichos biflorus Linn.) is a known antilithiatic, hypolipedemic and has free radical scavenging activity and increased production of reactive oxygen species play a role in pathophysiological mechanisms that trigger diabetic complications.

Aim: To see the effect of daily oral feeding of D.biflorous on nephropathy and retinopathy in streptozotocin (STZ) induced-diabetic rats.

Materials and methods: A total of 24 healthy rats were randomly grouped into controls, diabetic and diabetic on Dolichos. Diabetes was induced by a single dose of STZ (55 mg/kg) and animals were given prepared food and water ad libitum. Dolichos was orally given at 300 mg/kg/day to rats in diabetic on Dolichos group for next 30 days. Fasting blood glucose levels was monitored at beginning and at the end of the experiment while assessment of serum creatinine levels and histopathological study of kidney and retina was carried only at the end of the experiment. Statistical differences between groups were analyzed using analysis of variance followed by, Bonferroni test as posthoc test.

Results: Results indicated improvement in serum creatinine levels and reduced glomerular sclerosing and Bowman's space with interstitial alterations and significantly reduced renal hypertrophy in diabetic rat son Dolichos diabetic rats (P < 0.001). Retinal layers showed inconsistent improvement in the width of the neuronal layers and decreased vacuolization of plexiform layers and retinal vessel density.

Conclusion: D. biflorus at doses of 300 mg/kg/day for 30 days resulted in gradual but significant decreased diabetic nephropathy.

No MeSH data available.


Related in: MedlinePlus