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Antioxidant potential and its relationship with polyphenol content and degree of polymerization in Opuntia elatior Mill. fruits.

Itankar PR, Sontakke VA, Tauqeer M, Charde SS - Ayu (2014 Oct-Dec)

Bottom Line: The highest polyphenolic, FA, FO contents and degree of polymerization were found in EAOE.The increased antioxidant potential of EAOE and BFOE fractions over HAOE extract may be attributed to the purification achieved by fractionation of the extract which in turn resulted in an increase in the degree of polymerization and segregation of secondary metabolites.The fruit of O. elatior can be used as the best alternative for synthetic antioxidants.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy and Phytochemistry, University Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, India.

ABSTRACT

Background: Opuntia elatior Mill. (Nagaphani) fruits are traditionally recommended as an expectorant, remedy for whooping cough, asthma, gonorrhea, ulcers, tumors, in the treatment of diarrhea and syphilis. Many of these diseases are allied with oxidative stress caused by free radicals. Thus, current research is directed towards finding naturally-occurring antioxidants of plant origin.

Aim: To evaluate antioxidant potential of hydro-alcoholic extract of the O. elatior fruits (HAOE) and its fractions.

Materials and methods: Using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide radical scavenging assay, total polyphenolic, flavonoid (FA), flavanone (FO) contents and degree of polymerization in relation with its antioxidant activity were examined.

Results: The experimental data indicated that the HAOE, ethyl acetate (EAOE) and butanol (BFOE) soluble fractions have shown significant antioxidant activity. The highest polyphenolic, FA, FO contents and degree of polymerization were found in EAOE. The scavenging potential was in the order of Ascorbic Acid > EAOE > BFOE > HAOE > BIOE, where ascorbic acid was used as a positive control. The increased antioxidant potential of EAOE and BFOE fractions over HAOE extract may be attributed to the purification achieved by fractionation of the extract which in turn resulted in an increase in the degree of polymerization and segregation of secondary metabolites.

Conclusion: The fruit of O. elatior can be used as the best alternative for synthetic antioxidants.

No MeSH data available.


Related in: MedlinePlus

Graphical representation of antioxidant activity for the extracts/fractions. (a) 1,1 - diphenyl - 2 - picrylhydrazyl radical scavenging assay; (b) nitric oxide radical scavenging assay
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Figure 3: Graphical representation of antioxidant activity for the extracts/fractions. (a) 1,1 - diphenyl - 2 - picrylhydrazyl radical scavenging assay; (b) nitric oxide radical scavenging assay

Mentions: While comparing with the standard, amongst the extract and its fractions, EAOE has shown most prominent antioxidant activity with the IC50 value 44.52 ± 0.531 μg/ml. The scavenging activity of ascorbic acid, a known antioxidant, used as positive control, was higher and scavenging potential was found to be in the following order of ascorbic acid > EAOE > BFOE > HAOE > BIOE [Figure 3a].


Antioxidant potential and its relationship with polyphenol content and degree of polymerization in Opuntia elatior Mill. fruits.

Itankar PR, Sontakke VA, Tauqeer M, Charde SS - Ayu (2014 Oct-Dec)

Graphical representation of antioxidant activity for the extracts/fractions. (a) 1,1 - diphenyl - 2 - picrylhydrazyl radical scavenging assay; (b) nitric oxide radical scavenging assay
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492028&req=5

Figure 3: Graphical representation of antioxidant activity for the extracts/fractions. (a) 1,1 - diphenyl - 2 - picrylhydrazyl radical scavenging assay; (b) nitric oxide radical scavenging assay
Mentions: While comparing with the standard, amongst the extract and its fractions, EAOE has shown most prominent antioxidant activity with the IC50 value 44.52 ± 0.531 μg/ml. The scavenging activity of ascorbic acid, a known antioxidant, used as positive control, was higher and scavenging potential was found to be in the following order of ascorbic acid > EAOE > BFOE > HAOE > BIOE [Figure 3a].

Bottom Line: The highest polyphenolic, FA, FO contents and degree of polymerization were found in EAOE.The increased antioxidant potential of EAOE and BFOE fractions over HAOE extract may be attributed to the purification achieved by fractionation of the extract which in turn resulted in an increase in the degree of polymerization and segregation of secondary metabolites.The fruit of O. elatior can be used as the best alternative for synthetic antioxidants.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy and Phytochemistry, University Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, India.

ABSTRACT

Background: Opuntia elatior Mill. (Nagaphani) fruits are traditionally recommended as an expectorant, remedy for whooping cough, asthma, gonorrhea, ulcers, tumors, in the treatment of diarrhea and syphilis. Many of these diseases are allied with oxidative stress caused by free radicals. Thus, current research is directed towards finding naturally-occurring antioxidants of plant origin.

Aim: To evaluate antioxidant potential of hydro-alcoholic extract of the O. elatior fruits (HAOE) and its fractions.

Materials and methods: Using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide radical scavenging assay, total polyphenolic, flavonoid (FA), flavanone (FO) contents and degree of polymerization in relation with its antioxidant activity were examined.

Results: The experimental data indicated that the HAOE, ethyl acetate (EAOE) and butanol (BFOE) soluble fractions have shown significant antioxidant activity. The highest polyphenolic, FA, FO contents and degree of polymerization were found in EAOE. The scavenging potential was in the order of Ascorbic Acid > EAOE > BFOE > HAOE > BIOE, where ascorbic acid was used as a positive control. The increased antioxidant potential of EAOE and BFOE fractions over HAOE extract may be attributed to the purification achieved by fractionation of the extract which in turn resulted in an increase in the degree of polymerization and segregation of secondary metabolites.

Conclusion: The fruit of O. elatior can be used as the best alternative for synthetic antioxidants.

No MeSH data available.


Related in: MedlinePlus