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Pharmacognostical and high performance thin layer chromatography studies on leaves of Clerodendrum infortunatum L.

Verma S, Gupta R - Ayu (2014 Oct-Dec)

Bottom Line: Clerodendrum infortunatum L. commonly known as Bhant plays a significant role in Indian System of Medicine, that is, Ayurveda, due to its medicinal properties.HPTLC studies revealed that the amount of gallic acid in the crude powder of test sample were high (0.244 mg/g) in comparison to tyrosine (0.081 mg/g).The data generated would be of significant use for the authentication of drug and would also serve as a reference for the standardization and quality control of C. infortunatum.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, School of Pharmacy, Babu Banarasi Das University, Lucknow, Uttar Pradesh, India.

ABSTRACT

Background: Clerodendrum infortunatum L. commonly known as Bhant plays a significant role in Indian System of Medicine, that is, Ayurveda, due to its medicinal properties. It grows easily in waste places of all areas of India, Bangladesh, and Myanmar.

Aim: The present study was carried out with a view to lay down its pharmacognostic standards along with high-performance thin layer chromatography (HPTLC) studies in order to document/validate its therapeutic benefits.

Materials and methods: In this research, leaves of C. infortunatum were subjected to pharmacognostic evaluation parameters such as macroscopy, quantitative microscopy, physicochemical studies, phytochemical screening. HPTLC has been developed for detection and quantification of gallic acid and tyrosine in C. infortunatum. Increasing serial dilutions of reference standard gallic acid (20-100 μg/mL) and tyrosine (20-100 μg/mL) were scanned at 254 nm and 280 nm, respectively.

Results: Microscopy of leaf revealed the presence of anisocytic stomata, sclereids, glandular and covering trichome, and prisms of calcium oxalate crystal. The total ash, water-soluble, and acid insoluble ash values of leaves were 9.95%, 2.15%, and 0.70%, respectively. The maximum extractive value of crude powder was in the water. HPTLC studies revealed that the amount of gallic acid in the crude powder of test sample were high (0.244 mg/g) in comparison to tyrosine (0.081 mg/g).

Conclusion: The data generated would be of significant use for the authentication of drug and would also serve as a reference for the standardization and quality control of C. infortunatum.

No MeSH data available.


Calibration curve for standard gallic acid
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Figure 15: Calibration curve for standard gallic acid

Mentions: The identity of the gallic acid and tyrosine bands in sample chromatograms was confirmed by the chromatogram obtained from the test sample with that obtained from the reference standard solution and by comparing retention factors of gallic acid and tyrosine from sample and standard solutions. The peak corresponding to gallic acid and tyrosine from the sample solution had same retention factor as that from the gallic acid and tyrosine standard (Rf 0.91 and Rf 0.88, respectively). The chromatograms of standard gallic acid [Figure 9] and tyrosine [Figure 10] and that of gallic acid and tyrosine in leaves of C. infortunatum were shown in the Figure 11a and b. The AUC obtained for various tracks of gallic acid and tyrosine were enumerated in Tables 4 and 5. The three-dimension spectra of all tracks of gallic acid and tyrosine scanned at 254 nm and 280 nm, respectively are shown in Figure 12a and b. Spectral comparison of gallic acid and tyrosine (reference standard) with gallic acid and tyrosine present in test samples is shown in Figure 13a and b, respectively. The calibration curve was linear in the range of 20–100 μg/mL, as illustrated in Figures 14 and 15. From the regression equation y = 1261.5× +830.35 and y = 1015.7× +19355, the concentration of gallic acid and tyrosine in test sample was found to be 5.588 μg/mL and 5.396 μg/mL, respectively. The estimated value on per gram basis of the drug was about 0.244 mg/g and 0.081 mg/g of leaves powder respectively.


Pharmacognostical and high performance thin layer chromatography studies on leaves of Clerodendrum infortunatum L.

Verma S, Gupta R - Ayu (2014 Oct-Dec)

Calibration curve for standard gallic acid
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4492027&req=5

Figure 15: Calibration curve for standard gallic acid
Mentions: The identity of the gallic acid and tyrosine bands in sample chromatograms was confirmed by the chromatogram obtained from the test sample with that obtained from the reference standard solution and by comparing retention factors of gallic acid and tyrosine from sample and standard solutions. The peak corresponding to gallic acid and tyrosine from the sample solution had same retention factor as that from the gallic acid and tyrosine standard (Rf 0.91 and Rf 0.88, respectively). The chromatograms of standard gallic acid [Figure 9] and tyrosine [Figure 10] and that of gallic acid and tyrosine in leaves of C. infortunatum were shown in the Figure 11a and b. The AUC obtained for various tracks of gallic acid and tyrosine were enumerated in Tables 4 and 5. The three-dimension spectra of all tracks of gallic acid and tyrosine scanned at 254 nm and 280 nm, respectively are shown in Figure 12a and b. Spectral comparison of gallic acid and tyrosine (reference standard) with gallic acid and tyrosine present in test samples is shown in Figure 13a and b, respectively. The calibration curve was linear in the range of 20–100 μg/mL, as illustrated in Figures 14 and 15. From the regression equation y = 1261.5× +830.35 and y = 1015.7× +19355, the concentration of gallic acid and tyrosine in test sample was found to be 5.588 μg/mL and 5.396 μg/mL, respectively. The estimated value on per gram basis of the drug was about 0.244 mg/g and 0.081 mg/g of leaves powder respectively.

Bottom Line: Clerodendrum infortunatum L. commonly known as Bhant plays a significant role in Indian System of Medicine, that is, Ayurveda, due to its medicinal properties.HPTLC studies revealed that the amount of gallic acid in the crude powder of test sample were high (0.244 mg/g) in comparison to tyrosine (0.081 mg/g).The data generated would be of significant use for the authentication of drug and would also serve as a reference for the standardization and quality control of C. infortunatum.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacognosy, School of Pharmacy, Babu Banarasi Das University, Lucknow, Uttar Pradesh, India.

ABSTRACT

Background: Clerodendrum infortunatum L. commonly known as Bhant plays a significant role in Indian System of Medicine, that is, Ayurveda, due to its medicinal properties. It grows easily in waste places of all areas of India, Bangladesh, and Myanmar.

Aim: The present study was carried out with a view to lay down its pharmacognostic standards along with high-performance thin layer chromatography (HPTLC) studies in order to document/validate its therapeutic benefits.

Materials and methods: In this research, leaves of C. infortunatum were subjected to pharmacognostic evaluation parameters such as macroscopy, quantitative microscopy, physicochemical studies, phytochemical screening. HPTLC has been developed for detection and quantification of gallic acid and tyrosine in C. infortunatum. Increasing serial dilutions of reference standard gallic acid (20-100 μg/mL) and tyrosine (20-100 μg/mL) were scanned at 254 nm and 280 nm, respectively.

Results: Microscopy of leaf revealed the presence of anisocytic stomata, sclereids, glandular and covering trichome, and prisms of calcium oxalate crystal. The total ash, water-soluble, and acid insoluble ash values of leaves were 9.95%, 2.15%, and 0.70%, respectively. The maximum extractive value of crude powder was in the water. HPTLC studies revealed that the amount of gallic acid in the crude powder of test sample were high (0.244 mg/g) in comparison to tyrosine (0.081 mg/g).

Conclusion: The data generated would be of significant use for the authentication of drug and would also serve as a reference for the standardization and quality control of C. infortunatum.

No MeSH data available.