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Development and performance analysis of Si-CaP/fine particulate bone powder combined grafts for bone regeneration.

Sun C, Tian Y, Xu W, Zhou C, Xie H, Wang X - Biomed Eng Online (2015)

Bottom Line: However, when applied individually, these two materials fall short of an ideal substitute material.Si-CaP/FPBP combined grafts was found to produce conditions of alkaline pH levels compared to FPBP, and scaffold surface morphology conducive to bone cell adhesion, proliferation, differentiation, tissue growth and transport of nutrients, while maintaining elasticity and mechanical strength and degradation at a rate closer to osteogenesis.Si-CaP/FPBP combined grafts was found to be superior to any of the two components individually.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, The Second Harbin City Hospital, Harbin, 150056, China. scl324@126.com.

ABSTRACT

Background: Although autogenous bone grafts as well as several bone graft substitute material have been used for some time, there is high demand for more efficient and less costly bone-substitute materials. Silicon-substituted calcium phosphates (Si-CaP) and fine particulate bone powder (FPBP) preparations have been previously shown to individually possess many of the required features of a bone graft substitute scaffold. However, when applied individually, these two materials fall short of an ideal substitute material. We investigated a new concept of combining Si-CaP with FPBP for improved performance in bone-repair.

Methods: We assessed Si-CaP/FPBP combined grafts in vitro, by measuring changes in pH, weight loss, water absorption and compressive strength over time.

Results: Si-CaP/FPBP combined grafts was found to produce conditions of alkaline pH levels compared to FPBP, and scaffold surface morphology conducive to bone cell adhesion, proliferation, differentiation, tissue growth and transport of nutrients, while maintaining elasticity and mechanical strength and degradation at a rate closer to osteogenesis.

Conclusion: Si-CaP/FPBP combined grafts was found to be superior to any of the two components individually.

No MeSH data available.


Related in: MedlinePlus

a X-ray diffraction spectrum of Si-CaP powder. b Far-infrared Fourier transform spectroscopy analysis of two batches (1, 2) of Si-CaP powder sintered at 1,200°C for 4 h.
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Fig2: a X-ray diffraction spectrum of Si-CaP powder. b Far-infrared Fourier transform spectroscopy analysis of two batches (1, 2) of Si-CaP powder sintered at 1,200°C for 4 h.

Mentions: X-ray diffraction analysis showed that synthesized Si-CaP was homogenous and single phase material with all peaks matching with standard Si-CaP diffraction peaks provided by the International Centre for Diffraction Data (Figure 2a). Far-infrared Fourier transform spectroscopy analysis of two batches of Si-CaP exhibited same absorbance spectrum with major bands at 1,125 and 692 cm−1, demonstrating the repeatability of this synthesis method (Figure 2b). The images obtained from FIB-SEM showed rod or spherical shapes of Si-CaP particles with small Si-CaP crystals clustered on the surface (Figure 3a–c). Nanoscale pores with irregular sizes and shapes, formed by Si-CaP crystal clusters, were present on particle surfaces. Macropores of 150–300 µm were evenly distributed and connected by micropores of 1–10 µm throughout the scaffolds. EDS point analysis detected 8% silicon in the Si-CaP (Figure 3d; Table 1). Additionally, as determined by liquid displacement methodology, the average porosity of Si-CaP was 74.144 ± 3.833%.Figure 2


Development and performance analysis of Si-CaP/fine particulate bone powder combined grafts for bone regeneration.

Sun C, Tian Y, Xu W, Zhou C, Xie H, Wang X - Biomed Eng Online (2015)

a X-ray diffraction spectrum of Si-CaP powder. b Far-infrared Fourier transform spectroscopy analysis of two batches (1, 2) of Si-CaP powder sintered at 1,200°C for 4 h.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4492003&req=5

Fig2: a X-ray diffraction spectrum of Si-CaP powder. b Far-infrared Fourier transform spectroscopy analysis of two batches (1, 2) of Si-CaP powder sintered at 1,200°C for 4 h.
Mentions: X-ray diffraction analysis showed that synthesized Si-CaP was homogenous and single phase material with all peaks matching with standard Si-CaP diffraction peaks provided by the International Centre for Diffraction Data (Figure 2a). Far-infrared Fourier transform spectroscopy analysis of two batches of Si-CaP exhibited same absorbance spectrum with major bands at 1,125 and 692 cm−1, demonstrating the repeatability of this synthesis method (Figure 2b). The images obtained from FIB-SEM showed rod or spherical shapes of Si-CaP particles with small Si-CaP crystals clustered on the surface (Figure 3a–c). Nanoscale pores with irregular sizes and shapes, formed by Si-CaP crystal clusters, were present on particle surfaces. Macropores of 150–300 µm were evenly distributed and connected by micropores of 1–10 µm throughout the scaffolds. EDS point analysis detected 8% silicon in the Si-CaP (Figure 3d; Table 1). Additionally, as determined by liquid displacement methodology, the average porosity of Si-CaP was 74.144 ± 3.833%.Figure 2

Bottom Line: However, when applied individually, these two materials fall short of an ideal substitute material.Si-CaP/FPBP combined grafts was found to produce conditions of alkaline pH levels compared to FPBP, and scaffold surface morphology conducive to bone cell adhesion, proliferation, differentiation, tissue growth and transport of nutrients, while maintaining elasticity and mechanical strength and degradation at a rate closer to osteogenesis.Si-CaP/FPBP combined grafts was found to be superior to any of the two components individually.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, The Second Harbin City Hospital, Harbin, 150056, China. scl324@126.com.

ABSTRACT

Background: Although autogenous bone grafts as well as several bone graft substitute material have been used for some time, there is high demand for more efficient and less costly bone-substitute materials. Silicon-substituted calcium phosphates (Si-CaP) and fine particulate bone powder (FPBP) preparations have been previously shown to individually possess many of the required features of a bone graft substitute scaffold. However, when applied individually, these two materials fall short of an ideal substitute material. We investigated a new concept of combining Si-CaP with FPBP for improved performance in bone-repair.

Methods: We assessed Si-CaP/FPBP combined grafts in vitro, by measuring changes in pH, weight loss, water absorption and compressive strength over time.

Results: Si-CaP/FPBP combined grafts was found to produce conditions of alkaline pH levels compared to FPBP, and scaffold surface morphology conducive to bone cell adhesion, proliferation, differentiation, tissue growth and transport of nutrients, while maintaining elasticity and mechanical strength and degradation at a rate closer to osteogenesis.

Conclusion: Si-CaP/FPBP combined grafts was found to be superior to any of the two components individually.

No MeSH data available.


Related in: MedlinePlus