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Development and performance analysis of Si-CaP/fine particulate bone powder combined grafts for bone regeneration.

Sun C, Tian Y, Xu W, Zhou C, Xie H, Wang X - Biomed Eng Online (2015)

Bottom Line: However, when applied individually, these two materials fall short of an ideal substitute material.Si-CaP/FPBP combined grafts was found to produce conditions of alkaline pH levels compared to FPBP, and scaffold surface morphology conducive to bone cell adhesion, proliferation, differentiation, tissue growth and transport of nutrients, while maintaining elasticity and mechanical strength and degradation at a rate closer to osteogenesis.Si-CaP/FPBP combined grafts was found to be superior to any of the two components individually.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, The Second Harbin City Hospital, Harbin, 150056, China. scl324@126.com.

ABSTRACT

Background: Although autogenous bone grafts as well as several bone graft substitute material have been used for some time, there is high demand for more efficient and less costly bone-substitute materials. Silicon-substituted calcium phosphates (Si-CaP) and fine particulate bone powder (FPBP) preparations have been previously shown to individually possess many of the required features of a bone graft substitute scaffold. However, when applied individually, these two materials fall short of an ideal substitute material. We investigated a new concept of combining Si-CaP with FPBP for improved performance in bone-repair.

Methods: We assessed Si-CaP/FPBP combined grafts in vitro, by measuring changes in pH, weight loss, water absorption and compressive strength over time.

Results: Si-CaP/FPBP combined grafts was found to produce conditions of alkaline pH levels compared to FPBP, and scaffold surface morphology conducive to bone cell adhesion, proliferation, differentiation, tissue growth and transport of nutrients, while maintaining elasticity and mechanical strength and degradation at a rate closer to osteogenesis.

Conclusion: Si-CaP/FPBP combined grafts was found to be superior to any of the two components individually.

No MeSH data available.


Related in: MedlinePlus

The Si-CaP/FPBP tube (a), and the: changes of pH (b), weight (c), water absorption ability (d), compression strength (e) and elastic modulus (f) after the scaffolds were soaked in DMEM medium for different time periods. n = 7. **p < 0.01 compared to Si-CaP group.
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Fig1: The Si-CaP/FPBP tube (a), and the: changes of pH (b), weight (c), water absorption ability (d), compression strength (e) and elastic modulus (f) after the scaffolds were soaked in DMEM medium for different time periods. n = 7. **p < 0.01 compared to Si-CaP group.

Mentions: To prepare Si-CaP/FPBP scaffolds, Si-CaP and FPBP were mixed at a 1:1 weight ratio and packed into 1.6 cm3 tubes (diameter: 0.92 cm, height: 2 cm). An example of the scaffold is shown in Figure 1a. Si-CaP, FPBP and Si-CaP/FPBP scaffolds were then subjected to in vitro degradation tests at 37°C. Experiments were divided into three groups: Si-CaP group, FPBP and Si-CaP/FPBP. Si-CaP tubes were filled with FPBP at a weight ratio of 1:1 (0.75 g of each component). Bone scaffolds were cultured in six well culture plates containing 15 ml DMEM culture medium (pH 7.4) (Invitrogen, USA). Bone scaffolds were incubated at 37°C for 3, 7, 14 and 28 days.Figure 1


Development and performance analysis of Si-CaP/fine particulate bone powder combined grafts for bone regeneration.

Sun C, Tian Y, Xu W, Zhou C, Xie H, Wang X - Biomed Eng Online (2015)

The Si-CaP/FPBP tube (a), and the: changes of pH (b), weight (c), water absorption ability (d), compression strength (e) and elastic modulus (f) after the scaffolds were soaked in DMEM medium for different time periods. n = 7. **p < 0.01 compared to Si-CaP group.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4492003&req=5

Fig1: The Si-CaP/FPBP tube (a), and the: changes of pH (b), weight (c), water absorption ability (d), compression strength (e) and elastic modulus (f) after the scaffolds were soaked in DMEM medium for different time periods. n = 7. **p < 0.01 compared to Si-CaP group.
Mentions: To prepare Si-CaP/FPBP scaffolds, Si-CaP and FPBP were mixed at a 1:1 weight ratio and packed into 1.6 cm3 tubes (diameter: 0.92 cm, height: 2 cm). An example of the scaffold is shown in Figure 1a. Si-CaP, FPBP and Si-CaP/FPBP scaffolds were then subjected to in vitro degradation tests at 37°C. Experiments were divided into three groups: Si-CaP group, FPBP and Si-CaP/FPBP. Si-CaP tubes were filled with FPBP at a weight ratio of 1:1 (0.75 g of each component). Bone scaffolds were cultured in six well culture plates containing 15 ml DMEM culture medium (pH 7.4) (Invitrogen, USA). Bone scaffolds were incubated at 37°C for 3, 7, 14 and 28 days.Figure 1

Bottom Line: However, when applied individually, these two materials fall short of an ideal substitute material.Si-CaP/FPBP combined grafts was found to produce conditions of alkaline pH levels compared to FPBP, and scaffold surface morphology conducive to bone cell adhesion, proliferation, differentiation, tissue growth and transport of nutrients, while maintaining elasticity and mechanical strength and degradation at a rate closer to osteogenesis.Si-CaP/FPBP combined grafts was found to be superior to any of the two components individually.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, The Second Harbin City Hospital, Harbin, 150056, China. scl324@126.com.

ABSTRACT

Background: Although autogenous bone grafts as well as several bone graft substitute material have been used for some time, there is high demand for more efficient and less costly bone-substitute materials. Silicon-substituted calcium phosphates (Si-CaP) and fine particulate bone powder (FPBP) preparations have been previously shown to individually possess many of the required features of a bone graft substitute scaffold. However, when applied individually, these two materials fall short of an ideal substitute material. We investigated a new concept of combining Si-CaP with FPBP for improved performance in bone-repair.

Methods: We assessed Si-CaP/FPBP combined grafts in vitro, by measuring changes in pH, weight loss, water absorption and compressive strength over time.

Results: Si-CaP/FPBP combined grafts was found to produce conditions of alkaline pH levels compared to FPBP, and scaffold surface morphology conducive to bone cell adhesion, proliferation, differentiation, tissue growth and transport of nutrients, while maintaining elasticity and mechanical strength and degradation at a rate closer to osteogenesis.

Conclusion: Si-CaP/FPBP combined grafts was found to be superior to any of the two components individually.

No MeSH data available.


Related in: MedlinePlus